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MTS reagent for cell proliferation assay. Tetrazolium inner salt is used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

- Purity > 98%
- Soluble in DPBS to 2 mg/ml

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Chemical Structure - MTS Reagent, cell proliferation assay reagent (AB223881), expandable thumbnail

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Key facts

CAS number
138169-43-4
Purity
> 98%
Form
Solid
Molecular weight
487.5 Da
Molecular formula
C20H17N5O6S2
PubChem identifier
2762693
Nature
Synthetic

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MTS reagent for cell proliferation assay. Tetrazolium inner salt is used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

- Purity > 98%
- Soluble in DPBS to 2 mg/ml

Key facts

Purity
> 98%
PubChem identifier
2762693
Solubility

Soluble in DPBS to 2 mg/ml.

Biochemical name
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium
Biological description

Tetrazolium inner salt used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

Canonical SMILES
CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=C(C=C3)S(=O)(=O)[O-])C4=CC(=CC=C4)OCC(=O)O)C
InChI
InChI=1S/C20H17N5O6S2/c1-12-13(2)32-20(21-12)25-23-19(14-4-3-5-16(10-14)31-11-18(26)27)22-24(25)15-6-8-17(9-7-15)33(28,29)30/h3-10H,11H2,1-2H3,(H-,26,27,28,29,30)
InChIKey
APRZHQXAAWPYHS-UHFFFAOYSA-N
IUPAC name
4-[5-[3-(carboxymethoxy)phenyl]-3-(4,5-dimethyl-1,3-thiazol-2-yl)tetrazol-3-ium-2-yl]benzenesulfonate

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark, Store under desiccating conditions

Notes

This product is manufactured by BioVision, an Abcam company and was previously called 2808 MTS Reagent. 2808-250 is the same size as the 250 mg size of ab223881.

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1 product image

  • Chemical Structure - MTS Reagent, cell proliferation assay reagent (ab223881), expandable thumbnail

    Chemical Structure - MTS Reagent, cell proliferation assay reagent (ab223881)

    2D chemical structure image of ab223881, MTS Reagent, cell proliferation assay reagent

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Product protocols

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