MTS Reagent, cell proliferation assay reagent

Key facts

CAS number

138169-43-4

Purity

>98%

Form

Solid

form

Molecular weight

487.5 Da

Molecular formula

C₂₀H₁₇N₅O₆S₂

PubChem

2762693

Nature

Synthetic

Solubility

Soluble in DPBS to 2 mg/ml

Biochemical name

3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium

Biological description

Tetrazolium inner salt used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.

Canonical smiles

CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=C(C=C3)S(=O)(=O)[O-])C4=CC(=CC=C4)OCC(=O)O)C

InChi

InChI=1S/C20H17N5O6S2/c1-12-13(2)32-20(21-12)25-23-19(14-4-3-5-16(10-14)31-11-18(26)27)22-24(25)15-6-8-17(9-7-15)33(28,29)30/h3-10H,11H2,1-2H3,(H-,26,27,28,29,30)

InChiKey

APRZHQXAAWPYHS-UHFFFAOYSA-N

IUPAC Name

4-[5-[3-(carboxymethoxy)phenyl]-3-(4,5-dimethyl-1,3-thiazol-2-yl)tetrazol-3-ium-2-yl]benzenesulfonate