Myricetin, irreversible TrxR inhibitor

• FuncS

Unknown

Functional Studies - Myricetin, irreversible TrxR inhibitor (AB120721)

PC 12 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of myricetin (ab120721). Increased expression of CaMKII (phospho T286) (ab32678) in PC 12 cells correlates with an increase in myricetin concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab32678 at 1/500 dilution and ab52476 at 1/500 dilution overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - Myricetin, irreversible TrxR inhibitor (AB120721)

2D chemical structure image of ab120721, Myricetin, irreversible TrxR inhibitor