MW 272.26 g/mol, Purity >98%.
Citrus flavonoid. Antioxidant, anti-inflammatory. Affects various signaling pathways in vitro and in vivo. Crosses the blood-brain barrier.
MW 272.26 g/mol, Purity >98%.
Citrus flavonoid. Antioxidant, anti-inflammatory. Affects various signaling pathways in vitro and in vivo. Crosses the blood-brain barrier.
Soluble in DMSO to 100 mM.
Soluble in ethanol to 100 mM.
Citrus flavonoid. Antioxidant, anti-inflammatory. Affects various signaling pathways in vitro and in vivo. Crosses the blood-brain barrier.
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2D chemical structure image of ab120958, Naringenin, Antioxidant
Serum starved HepG2 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (±)-naringenin (ab120958). Increased expression of p38 (phospho T180 + Y182) (Anti-p38 (phospho T180 + Y182) antibody [M139] ab45381) in HepG2 cells correlates with an increase in (±)-naringenin concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with Anti-p38 (phospho T180 + Y182) antibody [M139] ab45381 at 1 μg /ml and Anti-beta Actin antibody ab8227 at 1/1000 dilution overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (Goat Anti-Mouse IgG H&L (HRP) preadsorbed ab97040) at 1/10000 and visualised using ECL development solution.
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