NBQX, AMPA / kainate antagonist
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(135 Publications)
MW 336.28 Da, Purity >99%. Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).
View Alternative Names
10q23del, 5 AMP activated protein kinase subunit gamma 3, 5''-AMP-activated protein kinase subunit gamma-3, AAKG3_HUMAN, ACTR-I, ACVR1A, ACVR1_HUMAN, ACVRLK 3, ACVRLK2, ALK 6, ALK-2, ALK-3, AMPA 1, AMPA 2, AMPA 3, AMPA 4, AMPA-selective glutamate receptor 1, AMPA-selective glutamate receptor 2, AMPA-selective glutamate receptor 3, AMPA-selective glutamate receptor 4, AMPK gamma 3 chain, AMPK gamma3, AMPK subunit gamma-3, AMPKG3, Activin A receptor type I, Activin A receptor type II like kinase 2, Activin A receptor type II like kinase 3, Activin receptor like kinase 6, Activin receptor type I, Activin receptor type-1, Activin receptor-like kinase 2, Activin receptor-like kinase 3, Acvrlk6, BMP type-1A receptor, BMP type-1B receptor, BMPR IB, BMR1A_HUMAN, BMR1B_HUMAN, BR 1a, BR 1b, Bmpr, Bone morphogenetic protein receptor type IA, Bone morphogenetic protein receptor type IA precursor, Bone morphogenetic protein receptor type IB, Bone morphogenetic protein receptor type-1A, Bone morphogenetic protein receptor type-1B, CD 292, CD292 antigen, CDw 293, CDw293 antigen, CFK 43a, EC 2.7.11.30, EEA3, Excitatory amino acid receptor 1, Excitatory amino acid receptor 2, Excitatory amino acid receptor 3, Excitatory amino acid receptor 4, Excitatory amino acid receptor 5, FOP, GLR 6, GLR 7, GLR5, GLUH1, GLUK3, GLUK6, GLUR4C, GRIA1_HUMAN, GRIA2_HUMAN, GRIA3_HUMAN, GRIA4_HUMAN, GRIK, GRIK1_HUMAN, GRIK2 protein, GRIK2_HUMAN, GRIK3_HUMAN, GRIK4_HUMAN, GRIK5_HUMAN, GluA 4, GluA1, GluA2, GluA3, GluK2, GluK4, GluK5, GluR 7a, GluR-1, GluR-2, GluR-3, GluR-4, GluR-5, GluR-6, GluR-7, GluR-A, GluR-B, GluR-C, GluR-D, GluR-K1, GluR-K2, GluR-K3, GluRgamma2, Glutamate ionotropic receptor AMPA type subunit 3, Glutamate receptor, Glutamate receptor 1, Glutamate receptor 2, Glutamate receptor 3, Glutamate receptor 4, Glutamate receptor 5, Glutamate receptor 6, Glutamate receptor 7, Glutamate receptor C, Glutamate receptor KA 1precursor, Glutamate receptor KA-1, Glutamate receptor KA-2, Glutamate receptor ionotrophic AMPA 3, Glutamate receptor ionotrophic AMPA 4, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic AMPA 2, Glutamate receptor ionotropic kainate 1, Glutamate receptor ionotropic kainate 2, Glutamate receptor ionotropic kainate 3, Glutamate receptor ionotropic kainate 4, Glutamate receptor ionotropic kainate 4 precursor, Glutamate receptor subunit 3, Glutamate receptor, ionotropic kainate 5 [Precursor], Glutamate receptor, ionotropic, AMPA 3, Glutamate receptor, ionotropic, kainate 5, Glutamate receptor, ionotropic, kainate 5 (gamma 2), HBGR1, HBGR2, Human glutamate receptor GLUR5, Hydroxyalkyl protein kinase, Ionotrophic Glutamate Receptor, Ionotropic Glutamate receptor 4, KA2, MGC118086, MGC133252, MRT6, MRX94, OTTHUMP00000045951, OTTHUMP00000096569, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243, OTTHUMP00000231881, PRKAG3, Protein kinase AMP activated gamma 3 non catalytic subunit, SKR 5, SKR1, Serine threonine protein kinase receptor R5, Serine threonine protein kinase receptor R5 precursor, Serine/threonine receptor kinase, Serine/threonine-protein kinase receptor R1, Serine/threonine-protein kinase receptor R5, TGF-B superfamily receptor type I, TSR-I, alk6tr, bA487F5.1, dJ1171F9.1, glutamate receptor form A, glutamate receptor form B, glutamate receptor form C, glutamate receptor form D, glutamate receptor form E, iGlu5, ionotropic kainate 1, ionotropic kainate 2, ionotropic kainate 3, ionotropic kainate 4, ionotropic kainate 5, zALK 6, zBMPR IA
- FuncS
Unknown
Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)
ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.
- FuncS
PubMed
Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)
A - Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom).
B - Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom)
Both cells were held at −68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 μm D-AP5 and 10 μm NBQX (ab120045, traces in red). Each trace represents an average of 10–20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar : (A) 50 μm, (B) 20 μm
Credit : Barker M et al. PLoS One. 2012; 7(5) : e35955. 10.1371/journal.pone.0035955
Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
- Chemical Structure
Lab
Chemical Structure - NBQX, AMPA / kainate antagonist (AB120045)
2D chemical structure image of ab120045, NBQX, AMPA / kainate antagonist
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
These receptors play an important role in synaptic communication and plasticity influencing processes like learning and memory formation. The GluA3 and GluK2 subunits are integral parts of the receptor complexes that also include other subunits such as GluA4 and GluK5. These receptors dynamically regulate the synaptic strength by their assembly into tetrameric structures determining excitatory synaptic responses. The diversity in subunit composition allows for precise modulation and response to synaptic activity.
Pathways
These receptors participate in key signaling cascades such as the glutamatergic and calcium signaling pathways. They directly interact with other proteins including scaffolding proteins like PSD-95 that link them to downstream effectors in these pathways. In the calcium signaling pathway the opening of these receptors leads to calcium influx which is significant for activating downstream signaling molecules including calmodulin-dependent protein kinases which modulate synaptic strength and plasticity.
Publications (135)
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STAR protocols 5:103255 PubMed39146190
2024
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Cell reports methods 3:100544 PubMed37671014
2023
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Cell reports 42:112039 PubMed36749664
2023
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The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5966-5990 PubMed35710623
2022
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Cell reports 39:110822 PubMed35584670
2022
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eLife 9: PubMed32602839
2020
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Journal of neurophysiology 123:2449-2464 PubMed32401131
2020
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eLife 8: PubMed31364987
2019
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FASEB journal : official publication of the Federa 33:5287-5299 PubMed30698461
2019
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Neurobiology of learning and memory 155:65-77 PubMed29953948
2018
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Product promise
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