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AB120045

NBQX, AMPA / kainate antagonist

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(135 Publications)

MW 336.28 Da, Purity >99%. Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).

View Alternative Names

10q23del, 5 AMP activated protein kinase subunit gamma 3, 5''-AMP-activated protein kinase subunit gamma-3, AAKG3_HUMAN, ACTR-I, ACVR1A, ACVR1_HUMAN, ACVRLK 3, ACVRLK2, ALK 6, ALK-2, ALK-3, AMPA 1, AMPA 2, AMPA 3, AMPA 4, AMPA-selective glutamate receptor 1, AMPA-selective glutamate receptor 2, AMPA-selective glutamate receptor 3, AMPA-selective glutamate receptor 4, AMPK gamma 3 chain, AMPK gamma3, AMPK subunit gamma-3, AMPKG3, Activin A receptor type I, Activin A receptor type II like kinase 2, Activin A receptor type II like kinase 3, Activin receptor like kinase 6, Activin receptor type I, Activin receptor type-1, Activin receptor-like kinase 2, Activin receptor-like kinase 3, Acvrlk6, BMP type-1A receptor, BMP type-1B receptor, BMPR IB, BMR1A_HUMAN, BMR1B_HUMAN, BR 1a, BR 1b, Bmpr, Bone morphogenetic protein receptor type IA, Bone morphogenetic protein receptor type IA precursor, Bone morphogenetic protein receptor type IB, Bone morphogenetic protein receptor type-1A, Bone morphogenetic protein receptor type-1B, CD 292, CD292 antigen, CDw 293, CDw293 antigen, CFK 43a, EC 2.7.11.30, EEA3, Excitatory amino acid receptor 1, Excitatory amino acid receptor 2, Excitatory amino acid receptor 3, Excitatory amino acid receptor 4, Excitatory amino acid receptor 5, FOP, GLR 6, GLR 7, GLR5, GLUH1, GLUK3, GLUK6, GLUR4C, GRIA1_HUMAN, GRIA2_HUMAN, GRIA3_HUMAN, GRIA4_HUMAN, GRIK, GRIK1_HUMAN, GRIK2 protein, GRIK2_HUMAN, GRIK3_HUMAN, GRIK4_HUMAN, GRIK5_HUMAN, GluA 4, GluA1, GluA2, GluA3, GluK2, GluK4, GluK5, GluR 7a, GluR-1, GluR-2, GluR-3, GluR-4, GluR-5, GluR-6, GluR-7, GluR-A, GluR-B, GluR-C, GluR-D, GluR-K1, GluR-K2, GluR-K3, GluRgamma2, Glutamate ionotropic receptor AMPA type subunit 3, Glutamate receptor, Glutamate receptor 1, Glutamate receptor 2, Glutamate receptor 3, Glutamate receptor 4, Glutamate receptor 5, Glutamate receptor 6, Glutamate receptor 7, Glutamate receptor C, Glutamate receptor KA 1precursor, Glutamate receptor KA-1, Glutamate receptor KA-2, Glutamate receptor ionotrophic AMPA 3, Glutamate receptor ionotrophic AMPA 4, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic AMPA 2, Glutamate receptor ionotropic kainate 1, Glutamate receptor ionotropic kainate 2, Glutamate receptor ionotropic kainate 3, Glutamate receptor ionotropic kainate 4, Glutamate receptor ionotropic kainate 4 precursor, Glutamate receptor subunit 3, Glutamate receptor, ionotropic kainate 5 [Precursor], Glutamate receptor, ionotropic, AMPA 3, Glutamate receptor, ionotropic, kainate 5, Glutamate receptor, ionotropic, kainate 5 (gamma 2), HBGR1, HBGR2, Human glutamate receptor GLUR5, Hydroxyalkyl protein kinase, Ionotrophic Glutamate Receptor, Ionotropic Glutamate receptor 4, KA2, MGC118086, MGC133252, MRT6, MRX94, OTTHUMP00000045951, OTTHUMP00000096569, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243, OTTHUMP00000231881, PRKAG3, Protein kinase AMP activated gamma 3 non catalytic subunit, SKR 5, SKR1, Serine threonine protein kinase receptor R5, Serine threonine protein kinase receptor R5 precursor, Serine/threonine receptor kinase, Serine/threonine-protein kinase receptor R1, Serine/threonine-protein kinase receptor R5, TGF-B superfamily receptor type I, TSR-I, alk6tr, bA487F5.1, dJ1171F9.1, glutamate receptor form A, glutamate receptor form B, glutamate receptor form C, glutamate receptor form D, glutamate receptor form E, iGlu5, ionotropic kainate 1, ionotropic kainate 2, ionotropic kainate 3, ionotropic kainate 4, ionotropic kainate 5, zALK 6, zBMPR IA

3 Images
Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)
  • FuncS

Unknown

Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)

ab96379 staining MEK1 (phospho S298) in SK-N-SH cells treated with NBQX (ab120045), by ICC/IF. Decrease in MEK1 (phospho S298) expression correlates with increased concentration of NBQX, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120045 (NBQX) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96379 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)
  • FuncS

PubMed

Functional Studies - NBQX, AMPA / kainate antagonist (AB120045)

A - Photomicrograph of a DCN fusiform cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this fusiform cell while stimulating the LVN (bottom).

B - Photomicrograph of a DCN granule cell filled with lucifer yellow (top) and whole cell voltage clamp recording of this granule cell while stimulating the LVN (bottom)

Both cells were held at −68 mV and the LVN was stimulated at 0.3 Hz. Glutamatergic EPSCs are represented in black and are blocked by 50 μm D-AP5 and 10 μm NBQX (ab120045, traces in red). Each trace represents an average of 10–20 single traces. The arrowhead represents the artifact of stimulus that has been removed for clarity. Scale bar : (A) 50 μm, (B) 20 μm

Credit : Barker M et al. PLoS One. 2012; 7(5) : e35955. 10.1371/journal.pone.0035955

Barker M et al. PLoS One. 2012; 7(5): e35955. 10.1371/journal.pone.0035955 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Chemical Structure - NBQX, AMPA / kainate antagonist (AB120045)
  • Chemical Structure

Lab

Chemical Structure - NBQX, AMPA / kainate antagonist (AB120045)

2D chemical structure image of ab120045, NBQX, AMPA / kainate antagonist

Key facts

CAS number

118876-58-7

Purity

>99%

Form

Solid

form

Molecular weight

336.28 Da

Molecular formula

C<sub>1</sub><sub>2</sub>H<sub>8</sub>N<sub>4</sub>O<sub>6</sub>S

PubChem

3272524

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

2,3-Dioxo-6-nitro-7-sulfamoylbenzo(f)quinoxaline

Biological description

Potent, selective and competitive AMPA/kainate receptor antagonist. Neuroprotective and anticonvulsant in vivo. Water-soluble form available - please see NBQX disodium salt (ab120046).

Canonical smiles

C1=CC2=C3C(=CC(=C2C(=C1)S(=O)(=O)N)[N+](=O)[O-])NC(=O)C(=O)N3

InChi

InChI=1S/C12H8N4O6S/c13-23(21,22)8-3-1-2-5-9(8)7(16(19)20)4-6-10(5)15-12(18)11(17)14-6/h1-4H,(H,14,17)(H,15,18)(H2,13,21,22)

InChiKey

UQNAFPHGVPVTAL-UHFFFAOYSA-N

IUPAC Name

6-nitro-2,3-dioxo-1,4-dihydrobenzo[f]quinoxaline-7-sulfonamide

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Glutamate Receptor 1 (AMPA subtype) and its related proteins such as BMPR1A BMPR1B and others like GRIK2/GluK2 and GluA3 function as ionotropic glutamate receptors that mediate excitatory synaptic transmission in the central nervous system. The mass of individual components like GluA3 is approximately 100-110 kDa. These receptors including kainate subtypes are widely expressed in the brain specifically in areas involved in learning and memory such as the hippocampus and the cerebral cortex. AMPA receptors work by allowing the flow of ions particularly sodium and calcium into the cells when glutamate binds to them resulting in neuron depolarization essential for synaptic plasticity and neurotransmission.
Biological function summary

These receptors play an important role in synaptic communication and plasticity influencing processes like learning and memory formation. The GluA3 and GluK2 subunits are integral parts of the receptor complexes that also include other subunits such as GluA4 and GluK5. These receptors dynamically regulate the synaptic strength by their assembly into tetrameric structures determining excitatory synaptic responses. The diversity in subunit composition allows for precise modulation and response to synaptic activity.

Pathways

These receptors participate in key signaling cascades such as the glutamatergic and calcium signaling pathways. They directly interact with other proteins including scaffolding proteins like PSD-95 that link them to downstream effectors in these pathways. In the calcium signaling pathway the opening of these receptors leads to calcium influx which is significant for activating downstream signaling molecules including calmodulin-dependent protein kinases which modulate synaptic strength and plasticity.

The dysfunction of these receptors has a strong connection to neurological diseases like epilepsy and neurodegenerative disorders such as Alzheimer's disease. Alterations in receptor function or subunit expression particularly those involving GluA3 and GluK2 have been observed in patients with these conditions. These proteins serve as potential targets for drug development and antagonists such as NBQX are being explored for therapeutic intervention. AMPA receptor dysregulation impacts synaptic stability and plasticity contributing to the disease pathophysiology.

Product protocols

Publications (135)

Recent publications for all applications. Explore the full list and refine your search

STAR protocols 5:103255 PubMed39146190

2024

Protocol for electrophysiological measurements of circadian changes in excitability in dentate granule cells from adult mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jose Carlos Gonzalez,Haeun Lee,Linda Overstreet-Wadiche

Cell reports methods 3:100544 PubMed37671014

2023

Functional imaging-guided cell selection for evolving genetically encoded fluorescent indicators.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Lin,Lihao Liu,Peng Zou

Cell reports 42:112039 PubMed36749664

2023

Circadian regulation of dentate gyrus excitability mediated by G-protein signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jose Carlos Gonzalez,Haeun Lee,Angela M Vincent,Angela L Hill,Lacy K Goode,Gwendalyn D King,Karen L Gamble,Jacques I Wadiche,Linda Overstreet-Wadiche

The Journal of neuroscience : the official journal of the Society for Neuroscience 42:5966-5990 PubMed35710623

2022

Modulating the Excitability of Olfactory Output Neurons Affects Whole-Body Metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Louis John Kolling,Roberta Tatti,Troy Lowry,Ashley M Loeven,James M Fadool,Debra Ann Fadool

Cell reports 39:110822 PubMed35584670

2022

Glutamate released by Cajal-Retzius cells impacts specific hippocampal circuits and behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Max Anstötz,Sun Kyong Lee,Gianmaria Maccaferri

eLife 9: PubMed32602839

2020

Parvalbumin interneurons provide spillover to newborn and mature dentate granule cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan J Vaden,Jose Carlos Gonzalez,Ming-Chi Tsai,Anastasia J Niver,Allison R Fusilier,Chelsea M Griffith,Richard H Kramer,Jacques I Wadiche,Linda Overstreet-Wadiche

Journal of neurophysiology 123:2449-2464 PubMed32401131

2020

BRAFV600E expression in neural progenitors results in a hyperexcitable phenotype in neocortical pyramidal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Roman U Goz,Gülcan Akgül,Joseph J LoTurco

eLife 8: PubMed31364987

2019

The readily-releasable pool dynamically regulates multivesicular release.

Applications

Unspecified application

Species

Unspecified reactive species

Jada H Vaden,Gokulakrishna Banumurthy,Eugeny S Gusarevich,Linda Overstreet-Wadiche,Jacques I Wadiche

FASEB journal : official publication of the Federa 33:5287-5299 PubMed30698461

2019

Overexpression of NEUROG2 and NEUROG1 in human embryonic stem cells produces a network of excitatory and inhibitory neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Congyi Lu,Xi Shi,Andrew Allen,David Baez-Nieto,Alexandria Nikish,Neville E Sanjana,Jen Q Pan

Neurobiology of learning and memory 155:65-77 PubMed29953948

2018

Anesthetics alleviate learning and memory impairment induced by electroconvulsive shock by regulation of NMDA receptor-mediated metaplasticity in depressive rats.

Applications

Unspecified application

Species

Unspecified reactive species

Li Ren,Xuechao Hao,Su Min,Jie Deng,Qibin Chen,Hengsheng Chen,Dawei Liu
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