MW 259.33 Da, Purity >99%. Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
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- International journal of molecular sciences 24:2023Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhimin Liao,Qing Zhu,Han HuangPubMed 37834141
- Stem cells translational medicine 11:1040-10512022Metabolic Maturation Increases Susceptibility to Hypoxia-induced Damage in Human iPSC-derived Cardiomyocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarijn C Peters,Renee G C Maas,Iris van Adrichem,Pieter A M Doevendans,Mark Mercola,Tomo Šarić,Jan W Buikema,Alain van Mil,Steven A J Chamuleau,Joost P G Sluijter,Anna P Hnatiuk,Klaus NeefPubMed 36018047
- International journal of molecular sciences 22:2021Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHien Lau,Shiri Li,Nicole Corrales,Samuel Rodriguez,Mohammadreza Mohammadi,Michael Alexander,Paul de Vos,Jonathan Rt LakeyPubMed 34445075
- American journal of cancer research 11:2062-20802021Necroptosis pathway blockage attenuates PFKFB3 inhibitor-induced cell viability loss and genome instability in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSiyuan Yan,Qianqian Li,Deru Zhang,Xiaowen Wang,Yang Xu,Cong Zhang,Dongli Guo,Yonghua BaoPubMed 34094669
- The FEBS journal 288:6476-64912021Caspase-9 acts as a regulator of necroptotic cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTamás Molnár,Petra Pallagi,Bálint Tél,Róbert Király,Eszter Csoma,Viktória Jenei,Zsófia Varga,Péter Gogolák,Anne Odile Hueber,Zoltán Máté,Ferenc Erdélyi,Gábor Szabó,Aladár Pettkó-Szandtner,Attila Bácsi,László Virág,József Maléth,Gábor KonczPubMed 33899329
- Xenotransplantation 28:e126672021The effects of necrostatin-1 on the in vitro development and function of young porcine islets over 14-day prolonged tissue culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHien Lau,Nicole Corrales,Samuel Rodriguez,Soomin Park,Mohammadreza Mohammadi,Shiri Li,Michael Alexander,Jonathan R T LakeyPubMed 33438288
- PloS one 15:e02435062020Dose-dependent effects of necrostatin-1 supplementation to tissue culture media of young porcine islets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHien Lau et. al.PubMed 33284818
- Antioxidants (Basel, Switzerland) 9:2020Toxicity of Necrostatin-1 in Parkinson's Disease Models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva Alegre-Cortés et. al.PubMed 32549347
- Arthritis research & therapy 22:242020Tumour necrosis factor alpha promotes secretion of 14-3-3η by inducing necroptosis in macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGulzhan Trimova et. al.PubMed 32051018
Key facts
- CAS number
4311-88-0
- Purity
> 99%
- Form
Solid
- Molecular weight
259.33 Da
- Molecular formula
C13H13N3OS
- PubChem identifier
2828334
- Nature
Synthetic
Alternative names
Cell death protein RIP, FLJ39204, OTTHUMP00000039163, RIPK1_HUMAN, Receptor (TNFRSF) interacting serine threonine kinase 1, Receptor TNFRSF interacting serine threonine kinase 1, Receptor interacting protein, Receptor interacting protein kinase 1, Receptor interacting serine threonine protein kinase 1, Receptor-interacting protein 1, Receptor-interacting serine/threonine-protein kinase 1, Rinp, Rip-1, Serine threonine protein kinase RIP, Serine/threonine-protein kinase RIP
Recommended products
MW 259.33 Da, Purity >99%. Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
Key facts
- CAS number
4311-88-0
- Purity
> 99%
- Form
Solid
- Molecular weight
259.33 Da
- Molecular formula
C13H13N3OS
- PubChem identifier
2828334
- Nature
Synthetic
- Solubility
Soluble in ethanol to 50 mM. Soluble in DMSO to 100 mM.
- Biochemical name
5-(1H-indol-3-ylmethyl)-3-methyl-2-thioxo-4-Imidazolidinone
- Biological description
Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
- Canonical SMILES
CN1C(=O)C(NC1=S)CC2=CNC3=CC=CC=C32
- InChI
InChI=1S/C13H13N3OS/c1-16-12(17)11(15-13(16)18)6-8-7-14-10-5-3-2-4-9(8)10/h2-5,7,11,14H,6H2,1H3,(H,15,18)
- InChIKey
TXUWMXQFNYDOEZ-UHFFFAOYSA-N
- IUPAC name
5-(1H-indol-3-ylmethyl)-3-methyl-2-sulfanylideneimidazolidin-4-one
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-20°C
- Appropriate long-term storage conditions
-20°C
- Storage information
Store under desiccating conditions, The product can be stored for up to 12 months
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RIP also known as Receptor-Interacting Protein or RIPK1 is a serine/threonine-protein kinase with a mass of approximately 74 kDa. It plays an important role in cell death and survival signaling pathways. RIP is expressed ubiquitously across various tissues indicating its importance in many cellular functions. The protein contains a kinase domain an intermediate domain for protein-protein interactions and a death domain which facilitates its involvement in apoptotic signaling processes.
- Biological function summary
Receptor-Interacting Protein Kinase 1 (RIPK1) participates in regulating both necroptosis and apoptosis distinguishing itself as an important mediator in cell death mechanisms. As part of the necrosome complex which includes RIPK3 and MLKL RIPK1 functions in necroptosis—a programmed form of necrosis. This characteristic involvement shows its dual role in maintaining cell fate decisions making it an integral part of immune response and inflammation control.
- Pathways
RIPK1 strongly associates with the TNF signaling pathway and NF-kB pathway. Its interaction with TNF receptor 1 (TNFR1) and consequent involvement with TRADD and TRAF2 mediates the signal transduction necessary for the activation of NF-kB leading to transcription of genes involved in survival and inflammation. This connection illustrates its capability to switch between promoting cell survival through NF-kB and facilitating cell death via necroptosis or apoptosis depending on cellular context and cues.
- Associated diseases and disorders
RIPK1 plays a significant role in conditions such as inflammatory diseases and neurodegenerative disorders. Its overactivation results in excessive cell death implicated in inflammatory conditions; necrostatin a necroptosis inhibitor targets RIPK1 to potentially mitigate this damage. Furthermore RIPK1's dysregulation links to Alzheimer's disease where it can interact with components like RIPK3 to exacerbate neurodegenerative processes. This relationship underlines the potential of targeting RIPK1 therapeutically to manage inflammation and neurodegeneration.
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2 product images
Chemical Structure - Necrostatin-1, necroptosis inhibitor (ab141053)
2D chemical structure image of ab141053, Necrostatin-1, necroptosis inhibitor
Yan et al PLoS One. 2018 Nov 21;13(11):e0207760. doi: 10.1371/journal.pone.0207760. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Necrostatin-1, necroptosis inhibitor (ab141053)
Necroptosis exists in PDLSCs.
TEM, a representative normal cell (the cell had a long fusiform, cell membrane structure was complete, the nucleus was round, nucleolus was obvious and center), b representative necroptosis cell (cell membrane ruptured, organelle disappeared, chromatin consolidated and agglutinated, presenting necrotic ultrastructural characteristics), c representative necrosis cell after Nec-1 inhibited (the cell membrane was intact, and the chromatin structure returned to normal).
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