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AB141053

Necrostatin-1, necroptosis inhibitor

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(20 Publications)

MW 259.33 Da, Purity >99%. Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.
2 Images
Functional Studies - Necrostatin-1, necroptosis inhibitor (AB141053)
  • FuncS

PubMed

Functional Studies - Necrostatin-1, necroptosis inhibitor (AB141053)

Necroptosis exists in PDLSCs.

TEM, a representative normal cell (the cell had a long fusiform, cell membrane structure was complete, the nucleus was round, nucleolus was obvious and center), b representative necroptosis cell (cell membrane ruptured, organelle disappeared, chromatin consolidated and agglutinated, presenting necrotic ultrastructural characteristics), c representative necrosis cell after Nec-1 inhibited (the cell membrane was intact, and the chromatin structure returned to normal).

Yan et al PLoS One. 2018 Nov 21;13(11):e0207760. doi: 10.1371/journal.pone.0207760. eCollection 2018. Fig 2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Chemical Structure - Necrostatin-1, necroptosis inhibitor (AB141053)
  • Chemical Structure

Lab

Chemical Structure - Necrostatin-1, necroptosis inhibitor (AB141053)

2D chemical structure image of ab141053, Necrostatin-1, necroptosis inhibitor

Key facts

CAS number

4311-88-0

Purity

>99%

Form

Solid

form

Molecular weight

259.33 Da

Molecular formula

C<sub>1</sub><sub>3</sub>H<sub>1</sub><sub>3</sub>N<sub>3</sub>OS

PubChem

2828334

Nature

Synthetic

Solubility

Soluble in ethanol to 50 mM

Soluble in DMSO to 100 mM

Biochemical name

5-(1H-indol-3-ylmethyl)-3-methyl-2-thioxo-4-Imidazolidinone

Biological description

Potent, specific necroptosis inhibitor (EC50 = 490 nM). Selective allosteric inhibitor of death domain receptor-associated adaptor kinase RIP1 in vitro (EC50 = 182 nM). Caspase independent, programmed necrosis pathway.

Canonical smiles

CN1C(=O)C(NC1=S)CC2=CNC3=CC=CC=C32

InChi

InChI=1S/C13H13N3OS/c1-16-12(17)11(15-13(16)18)6-8-7-14-10-5-3-2-4-9(8)10/h2-5,7,11,14H,6H2,1H3,(H,15,18)

InChiKey

TXUWMXQFNYDOEZ-UHFFFAOYSA-N

IUPAC Name

5-(1H-indol-3-ylmethyl)-3-methyl-2-sulfanylideneimidazolidin-4-one

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Complementary Products

Biochemicals

AB143839

Necrosulfonamide, necroptosis inhibitor

Chemical Structure - Necrosulfonamide, necroptosis inhibitor (AB143839)

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Primary Antibodies

AB187091

Anti-MLKL (phospho S358) antibody [EPR9514]

RabMAb|Recombinant|20ul selling size

Western blot - Anti-MLKL (phospho S358) antibody [EPR9514] (AB187091)

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  • 17
Biochemicals

AB146169

Ferrostatin 1, erastin-induced ferroptosis inhibitor

Chemical Structure - Ferrostatin 1, erastin-induced ferroptosis inhibitor (AB146169)

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Biochemicals

AB120382

Z-VAD(OH)-FMK, Irreversible general caspase inhibitor

Immunoprecipitation - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (AB120382)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RIP also known as Receptor-Interacting Protein or RIPK1 is a serine/threonine-protein kinase with a mass of approximately 74 kDa. It plays an important role in cell death and survival signaling pathways. RIP is expressed ubiquitously across various tissues indicating its importance in many cellular functions. The protein contains a kinase domain an intermediate domain for protein-protein interactions and a death domain which facilitates its involvement in apoptotic signaling processes.
Biological function summary

Receptor-Interacting Protein Kinase 1 (RIPK1) participates in regulating both necroptosis and apoptosis distinguishing itself as an important mediator in cell death mechanisms. As part of the necrosome complex which includes RIPK3 and MLKL RIPK1 functions in necroptosis—a programmed form of necrosis. This characteristic involvement shows its dual role in maintaining cell fate decisions making it an integral part of immune response and inflammation control.

Pathways

RIPK1 strongly associates with the TNF signaling pathway and NF-kB pathway. Its interaction with TNF receptor 1 (TNFR1) and consequent involvement with TRADD and TRAF2 mediates the signal transduction necessary for the activation of NF-kB leading to transcription of genes involved in survival and inflammation. This connection illustrates its capability to switch between promoting cell survival through NF-kB and facilitating cell death via necroptosis or apoptosis depending on cellular context and cues.

RIPK1 plays a significant role in conditions such as inflammatory diseases and neurodegenerative disorders. Its overactivation results in excessive cell death implicated in inflammatory conditions; necrostatin a necroptosis inhibitor targets RIPK1 to potentially mitigate this damage. Furthermore RIPK1's dysregulation links to Alzheimer's disease where it can interact with components like RIPK3 to exacerbate neurodegenerative processes. This relationship underlines the potential of targeting RIPK1 therapeutically to manage inflammation and neurodegeneration.
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Product protocols

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Publications (20)

Recent publications for all applications. Explore the full list and refine your search

Current oncology (Toronto, Ont.) 31:7390-7402 PubMed39590175

2024

Exploiting Integrin-αVβ3 to Enhance Radiotherapy Efficacy in Medulloblastoma via Ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Célia Gotorbe,Fabien Segui,William Echavidre,Jérôme Durivault,Thays Blanchard,Valérie Vial,Marina Pagnuzzi-Boncompagni,Rémy Villeneuve,Régis Amblard,Nicolas Garnier,Cécile Ortholan,Benjamin Serrano,Vincent Picco,Jacques Pouysségur,Milica Vucetic,Christopher Montemagno

Journal for immunotherapy of cancer 12: PubMed39500528

2024

In situ endoscopic photodynamic therapy combined with immature DC vaccination induces a robust T cell response against peritoneal carcinomatosis.

Applications

Unspecified application

Species

Unspecified reactive species

Charline Degavre,Anouk Lepez,Sebastien Ibanez,Clémence François,Katarzyna Głowacka,Céline Guilbaud,Florine Laloux-Morris,Hrag Esfahani,Davide Brusa,Caroline Bouzin,Olivier Feron

Cells 13: PubMed38201226

2024

Suppresses the Growth of Huh7-Derived Liver Cancer Stem Cells by Inducing Necroptosis and Apoptosis and Blocking c-MET Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Mikyoung Kwon,Hye Jin Jung

International journal of molecular sciences 24: PubMed37834141

2023

Involvement of IL-1β-Mediated Necroptosis in Neurodevelopment Impairment after Neonatal Sepsis in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhimin Liao,Qing Zhu,Han Huang

Stem cells translational medicine 11:1040-1051 PubMed36018047

2022

Metabolic Maturation Increases Susceptibility to Hypoxia-induced Damage in Human iPSC-derived Cardiomyocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Marijn C Peters,Renee G C Maas,Iris van Adrichem,Pieter A M Doevendans,Mark Mercola,Tomo Šarić,Jan W Buikema,Alain van Mil,Steven A J Chamuleau,Joost P G Sluijter,Anna P Hnatiuk,Klaus Neef

International journal of medicinal mushrooms 23:13-26 PubMed34375515

2021

Polysaccharides from Shiitake Culinary-Medicinal Mushroom Lentinus edodes (Agaricomycetes) Suppress pMLKL-Mediated Necroptotic Cell Death and Colitis in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Chidube Anthony Alagbaoso,Masashi Mizuno

International journal of molecular sciences 22: PubMed34445075

2021

Necrostatin-1 Supplementation to Islet Tissue Culture Enhances the In-Vitro Development and Graft Function of Young Porcine Islets.

Applications

Unspecified application

Species

Unspecified reactive species

Hien Lau,Shiri Li,Nicole Corrales,Samuel Rodriguez,Mohammadreza Mohammadi,Michael Alexander,Paul de Vos,Jonathan Rt Lakey

American journal of cancer research 11:2062-2080 PubMed34094669

2021

Necroptosis pathway blockage attenuates PFKFB3 inhibitor-induced cell viability loss and genome instability in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Siyuan Yan,Qianqian Li,Deru Zhang,Xiaowen Wang,Yang Xu,Cong Zhang,Dongli Guo,Yonghua Bao

The FEBS journal 288:6476-6491 PubMed33899329

2021

Caspase-9 acts as a regulator of necroptotic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Tamás Molnár,Petra Pallagi,Bálint Tél,Róbert Király,Eszter Csoma,Viktória Jenei,Zsófia Varga,Péter Gogolák,Anne Odile Hueber,Zoltán Máté,Ferenc Erdélyi,Gábor Szabó,Aladár Pettkó-Szandtner,Attila Bácsi,László Virág,József Maléth,Gábor Koncz

Xenotransplantation 28:e12667 PubMed33438288

2021

The effects of necrostatin-1 on the in vitro development and function of young porcine islets over 14-day prolonged tissue culture.

Applications

Unspecified application

Species

Unspecified reactive species

Hien Lau,Nicole Corrales,Samuel Rodriguez,Soomin Park,Mohammadreza Mohammadi,Shiri Li,Michael Alexander,Jonathan R T Lakey
View all publications
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