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MW 318.4 Da, Purity >95%. Photostable, lipophilic stain that strongly fluoresces in hydrophobic (lipid-rich) environments, but has minimal fluorescence in aqueous media. Commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.

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Key facts

CAS number
7385-67-3
Purity
> 95%
Form
Solid
Molecular weight
318.4 Da
Molecular formula
C20H18N2O2
PubChem identifier
65182
Nature
Synthetic
Excitation/Emission
Ex: 552nm, Em: 636nm

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Alternative names

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MW 318.4 Da, Purity >95%. Photostable, lipophilic stain that strongly fluoresces in hydrophobic (lipid-rich) environments, but has minimal fluorescence in aqueous media. Commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.

Key facts

Purity
> 95%
PubChem identifier
65182
Solubility

Soluble in DMSO.

Biochemical name
Nile red
Biological description

Photostable, lipophilic stain that strongly fluoresces in hydrophobic (lipid-rich) environments, but has minimal fluorescence in aqueous media. Commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.

Canonical SMILES
CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2
InChI
InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3
InChIKey
VOFUROIFQGPCGE-UHFFFAOYSA-N
IUPAC name
9-(diethylamino)benzo[a]phenoxazin-5-one
Excitation/Emission
Ex: 552nm, Em: 636nm

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions

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