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AB219403

Nile Red, lipophilic stain

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(2 Publications)

Nile Red (ab219403) is a lipophilic stain that strongly fluoresces in lipid-rich environments, but has minimal fluorescence in aqueous media. Commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry. MW 318.4, Purity >95%.

- Ex: 552 nm, Em: 636 nm
- Stains intracellular lipid droplets red

Key facts

CAS number

7385-67-3

Purity

>95%

Form

Solid

form

Molecular weight

318.4 Da

Molecular formula

C<sub>2</sub><sub>0</sub>H<sub>1</sub><sub>8</sub>N<sub>2</sub>O<sub>2</sub>

PubChem

65182

Nature

Synthetic

Solubility

Soluble in DMSO

Biochemical name

Nile red

Biological description

Photostable, lipophilic stain that strongly fluoresces in hydrophobic (lipid-rich) environments, but has minimal fluorescence in aqueous media. Commonly used for the detection of intracellular lipid droplets by fluorescence microscopy and flow cytofluorometry.

Canonical smiles

CCN(CC)C1=CC2=C(C=C1)N=C3C4=CC=CC=C4C(=O)C=C3O2

InChi

InChI=1S/C20H18N2O2/c1-3-22(4-2)13-9-10-16-18(11-13)24-19-12-17(23)14-7-5-6-8-15(14)20(19)21-16/h5-12H,3-4H2,1-2H3

InChiKey

VOFUROIFQGPCGE-UHFFFAOYSA-N

IUPAC Name

9-(diethylamino)benzo[a]phenoxazin-5-one

Excitation/Emission

Ex: 552nm, Em: 636nm

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Complementary Products

Assay Kits

AB228553

Nile Red Staining Kit

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Assay Kits

AB243377

Lipid Peroxidation Assay Kit (Cell-based)

Fluorescence Microscopy - Lipid Peroxidation Assay Kit (Cell-based) (AB243377)

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Assay Kits

AB150678

Oil Red O Stain Kit (Lipid Stain)

Histology - Oil Red O Stain Kit (Lipid Stain) (AB150678)

  • 5
  • 1
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions
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Product protocols

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed38251655

2024

Ferredoxin 1 is essential for embryonic development and lipid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Shakur Mohibi,Yanhong Zhang,Vivian Perng,Mingyi Chen,Jin Zhang,Xinbin Chen

Developmental cell 56:1000-1013.e6 PubMed33725482

2021

Mauve/LYST limits fusion of lysosome-related organelles and promotes centrosomal recruitment of microtubule nucleating proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Ramona Lattao,Hélène Rangone,Salud Llamazares,David M Glover
View all publications
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