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AB146262

Nitrotetrazolium blue chloride (NBT), Chromogenic substrate

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(7 Publications)

MW 817.64 g/mol. Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).
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Chemical Structure - Nitrotetrazolium blue chloride (NBT), Chromogenic substrate (AB146262)
  • Chemical Structure

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Chemical Structure - Nitrotetrazolium blue chloride (NBT), Chromogenic substrate (AB146262)

2D chemical structure image of ab146262, Nitrotetrazolium blue chloride (NBT), Chromogenic substrate

Key facts

CAS number

298-83-9

Form

Solid

form

Molecular weight

817.64 g/mol

Molecular formula

C<sub>4</sub><sub>0</sub>H<sub>3</sub><sub>0</sub>Cl<sub>2</sub>N<sub>1</sub><sub>0</sub>O<sub>6</sub>

Nature

Synthetic

Solubility

Soluble in water to 10 mM

Biological description

Chromogenic substrate for dehydrogenases and other oxidases that, like other tetrazolium compounds, can be reduced to produce a colored formazan derivative. Acts as an NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase. Used for detection of alkaline phosphatase in combination with 5-bromo-4-chloro-3-indoxyl phosphate (BCIP).

IUPAC Name

3,3'-[3,3'-Dimethoxy(1,1'-biphenyl)-4,4'-diyl]bis[2-(4-nitrophenyl)-5-phenyl-2H-tetrazolium dichloride

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Product protocols

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 17:336-364 PubMed39748059

2025

PlexinD1 is a driver and a therapeutic target in advanced prostate cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wei,Jing Wang,Wen Guan,Jingjing Li,Tianjie Pu,Eva Corey,Tzu-Ping Lin,Allen C Gao,Boyang Jason Wu

Life (Basel, Switzerland) 14: PubMed39337855

2024

Investigating the Effects of Chelidonic Acid on Oxidative Stress-Induced Premature Cellular Senescence in Human Skin Fibroblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Burcu Turkoglu,Banu Mansuroglu

Scientific reports 13:19552 PubMed37945711

2023

STAT3 phosphorylation at serine 727 activates specific genetic programs and promotes clear cell renal cell carcinoma (ccRCC) aggressiveness.

Applications

Unspecified application

Species

Unspecified reactive species

J Arévalo,I Campoy,M Durán,S Nemours,A Areny,M Vall-Palomar,C Martínez,G Cantero-Recasens,A Meseguer

Frontiers in pharmacology 13:1000278 PubMed36267290

2022

NQO1 protects against clioquinol toxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Jamuna Chhetri,Jem Dilek,Noel Davies,Glenn Jacobson,Robert Dallmann,Nuri Gueven

Neurobiology of disease 169:105737 PubMed35452786

2022

SOD1 mediates lysosome-to-mitochondria communication and its dysregulation by amyloid-β oligomers.

Applications

Unspecified application

Species

Unspecified reactive species

Andrés Norambuena,Xuehan Sun,Horst Wallrabe,Ruofan Cao,Naidi Sun,Evelyn Pardo,Nutan Shivange,Dora Bigler Wang,Lisa A Post,Heather A Ferris,Song Hu,Ammasi Periasamy,George S Bloom

Scientific reports 12:2354 PubMed35149730

2022

Relationship between oxidative stress and lifespan in Daphnia pulex.

Applications

Unspecified application

Species

Unspecified reactive species

Benedicth Ukhueduan,Charles Schumpert,Eunsuk Kim,Jeffry L Dudycha,Rekha C Patel

The Journal of biological chemistry 291:18016-29 PubMed27358405

2016

Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES.

Applications

Unspecified application

Species

Unspecified reactive species

Gaurab Sircar,Kuladip Jana,Angira Dasgupta,Sudipto Saha,Swati Gupta Bhattacharya
View all publications
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