NMDA, excitotoxic amino acid
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(19 Publications)
MW 147.13 Da, Purity >99%. Excitotoxic amino acid. Prototypic agonist at the ionotropic NMDA glutamate receptor which is involved in long-term potentiation, ischemia, and epilepsy.
View Alternative Names
AMPA 1, AMPA-selective glutamate receptor 1, AW490526, CHNG1, EB11, EC 3.4.21., EIEE27, EPND, FESD, GLUH1, GRIA1_HUMAN, GRIN 2A, GRIN 2B, GRIN3A, GRIN3B, GluA1, GluN1, GluN2A, GluN2C, GluN2D, GluR-1, GluR-A, GluR-K1, Glutamate Receptor Ionotropic N Methyl D Aspartate 2B, Glutamate Receptor Ionotropic N Methyl D Aspartate 2C, Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B, Glutamate Receptor Ionotropic N methyl D aspartate 3A, Glutamate [NMDA] receptor subunit epsilon-1, Glutamate [NMDA] receptor subunit epsilon-2, Glutamate [NMDA] receptor subunit epsilon-3, Glutamate [NMDA] receptor subunit epsilon-4, Glutamate [NMDA] receptor subunit zeta-1, Glutamate receptor, Glutamate receptor 1, Glutamate receptor ionotropic, Glutamate receptor ionotropic AMPA 1, Glutamate receptor ionotropic N methyl D aspartate 1, Glutamate receptor ionotropic N methyl D aspartate 2A, Glutamate receptor ionotropic N methyl D aspartate 3B, Glutamate receptor ionotropic NMDA2B, Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1, Glutamate receptor ionotropic, NMDA 2C, Glutamate receptor subunit epsilon 2, Glutamate receptor, ionotropic, N-methyl D-aspartate 2D, Glutamate receptor, ionotropic, NMDA2B (epsilon 2), Grin2c, Grin2d, HBGR1, HEPS_HUMAN, Hepsin, Hpn, LGR 3, LKS, MGC133252, MGC142178, MGC142180, MGC75129, MRD6, MRD8, N Methly D Aspartate Receptor Channel Subunit Epsilon 3, N methyl D asparate receptor channel subunit epsilon 2, N methyl D aspartate receptor channel subunit zeta 1, N methyl D aspartate receptor channel, subunit epsilon 1, N methyl D aspartate receptor subunit 2A, N methyl D aspartate receptor subunit 2B, N methyl D aspartate receptor subunit 2C, N methyl d aspartate receptor subunit 2D, N-methyl D-aspartate receptor subtype 2A, N-methyl D-aspartate receptor subtype 2B, N-methyl D-aspartate receptor subtype 2C, N-methyl D-aspartate receptor subtype 2D, N-methyl-D-aspartate receptor, N-methyl-D-aspartate receptor subunit 3, N-methyl-D-aspartate receptor subunit NR1, NMD-R1, NMDA 1, NMDA 2D, NMDA NR2B, NMDA receptor 1, NMDA receptor subtype 2A, NMDA receptor subunit 3A, NMDA receptor subunit 3B, NMDAR, NMDAR2C, NMDAR2D, NMDE1_HUMAN, NMDE2_HUMAN, NMDE3_HUMAN, NMDE4_HUMAN, NMDZ1_HUMAN, NR1, NR2A, NR2B, NR2C, NR2D, NR3, OTTHUMP00000041930, OTTHUMP00000160135, OTTHUMP00000160643, OTTHUMP00000165781, OTTHUMP00000174531, OTTHUMP00000224241, OTTHUMP00000224242, OTTHUMP00000224243, Serine protease hepsin, Serine protease hepsin catalytic chain, Serine protease hepsin non catalytic chain, Seven transmembrane helix receptor, TMPRSS 1, TSH Receptor, TSHR_HUMAN, Thyroid adenoma hyperfunctioning, Thyroid carcinoma with thyrotoxicosis, Thyroid stimulating hormone receptor, isoform 2, Thyroid-stimulating hormone receptor, Thyrotropin receptor, Thyrotropin receptor I, Thyrotropin receptor I, hTSHR I, Transmembrane protease serine 1, estrogen receptor binding CpG island, glutamate receptor ionotropic NMDA 2D, glutamate receptor ionotropic, NMDA 1, hNR 3, hNR2A, hTSHR I
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - NMDA, excitotoxic amino acid (AB120052)
ab55051 staining GABA B receptor 1 in SK-N-SH cells treated with NMDA (ab120052), by ICC/IF. Internalization of GABA B receptor 1 correlates with increased concentration of NMDA, as described in literature.
The cells were incubated at 37°C for 30 minutes in media containing different concentrations of ab120052 (NMDA) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab55051 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- Chemical Structure
Lab
Chemical Structure - NMDA, excitotoxic amino acid (AB120052)
2D chemical structure image of ab120052, NMDA, excitotoxic amino acid
- FuncS
PubMed
Functional Studies - NMDA, excitotoxic amino acid (AB120052)
Release of adenosine by depolarisation and agonists.
(Panel c) NMDA application also evoked neuronal depolarisation and firing accompanied by subsequent release of adenosine and inosine.
Sims et al PLoS One. 2013;8(1):e53814. doi: 10.1371/journal.pone.0053814. Epub 2013 Jan 11. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
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Supplementary information
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Biological function summary
These proteins participate in neurotransmission and signal transduction. NMDARs form part of a receptor complex interacting with various regulatory proteins facilitating calcium ion influx when activated by glutamate. This ion movement is essential for long-term potentiation and synaptic plasticity important for learning and memory. The TSH receptor functions in the thyroid hormone synthesis signaling pathway initiating cascades upon thyroid stimulating hormone binding. Meanwhile AMPA receptors mediate fast synaptic transmission primarily through sodium ion flux contributing significantly to excitatory neurotransmission in the brain.
Pathways
NMDARs are central to the glutamatergic pathway involving neurotransmitter release and various intracellular signaling mechanisms. This pathway intersects with the calcium/calmodulin-dependent protein kinase (CaMK) pathway. Proteins such as CaMKII associate with NMDARs impacting synaptic strength and excitability. The TSH receptor operates within the hypothalamic-pituitary-thyroid (HPT) axis influencing thyroid hormone levels. This receptor collaborates with other proteins like G-proteins to propagate intracellular signaling. AMPA receptors interact with NMDA receptors in synaptic transmission pathways influencing neuronal communication and plasticity.
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Publications (19)
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Neurobiology of disease 191:106408 PubMed38199274
2024
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Neural regeneration research 18:1124-1131 PubMed36255002
2022
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Biology of reproduction 107:916-927 PubMed35746896
2022
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Proceedings of the National Academy of Sciences of 117:25830-25839 PubMed32973097
2020
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Journal of neurochemistry 152:523-541 PubMed31376158
2019
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Journal of neuroscience research 96:391-406 PubMed29193273
2017
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Cell reports 19:2694-2706 PubMed28658618
2017
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Drosophila melanogaster
Scientific reports 7:46514 PubMed28425451
2017
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Antimicrobial agents and chemotherapy 60:168-79 PubMed26482305
2015
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Molecular psychiatry 20:959-73 PubMed25266126
2014
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