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AB120630

Nocodazole, Microtubule inhibitor

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(18 Publications)

MW 301.32 Da, Purity >98%. Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.

View Alternative Names

2810484G07Rik, A830040C14Rik, ABL, ABL proto oncogene 1 non receptor tyrosine kinase, ABL1_HUMAN, ACLS, AHC, AHCH, AHX, AI838772, AIS, ALS22, ANDR_HUMAN, AP-1, APOPAIN, AR, AR8, AW493413, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine protein kinase 1, Activator protein 1, Alpha-tubulin 1, Alpha-tubulin 3, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease), Antigen NY-CO-13, Apoptotic protease Mch-3, B ALPHA 1, BCC7, Bcr/c abl oncogene protein, Beta 4 tubulin, Beta 5 tubulin, Beta tubulin 1, class VI, Beta-tubulin, Beta2, CASP3_HUMAN, CASP7_HUMAN, CBP_HUMAN, CC3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, CMH-1, CPP-32, CPP32B, CREB-binding protein, Casp3a, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase 7, Caspase 7 apoptosis related cysteine peptidase, Caspase-3, Caspase-3 subunit p12, Caspase-7 subunit p11, Cellular tumor antigen p53, Class I beta tubulin, Class II beta tubulin isotype, Class IVb beta tubulin, Class VI beta tubulin, Cyclic AMP responsive enhancer binding protein, Cysteine protease CPP32, DAX 1, DHTR, DKFZp566F223, DNA binding protein, DNA replication inhibitor, DSS-AHC critical region on the X chromosome protein 1, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), Dosage sensitive sex reversal, Dystonia 4 torsion (autosomal dominant), EC 3.4.22.56, Enhancer Binding Protein AP1, FEOM3, FLJ11090, FLJ25113, FLJ92943, FLJ98847, GCPS, GEMI_HUMAN, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI family zinc finger 3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, GMNN, GTD, Geminin, Geminin DNA replication inhibitor, Glioma associated oncogene family zinc finger 3, H2 ALPHA, HHG, HUMARA, HYSP1, Hum a tub1, Hum a tub2, ICE-LAP3, ICE-like apoptotic protease 3, ICE3, JTK7, JUN protein, JUNC, JUN_HUMAN, Jun Activation Domain Binding Protein, Jun oncogene, Jun proto oncogene, KAT3A, KD, Kennedy disease (KD), LFS1, LICE, LICE2, LIS3, M(beta)1, M(beta)2, M(beta)3, M(beta)6, M40, MC1-R, MCH3, MGC104252, MGC112732, MGC117247, MGC16435, MGC171407, MGC55332, MGC8685, Mutant tumor protein 53, NF-E2-related factor 2, NF2L2_HUMAN, NR0B1_HUMAN, NR3C4, NRF2, NROB1, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, Nfe2l2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2, Nuclear hormone receptor, Nuclear receptor 0B1, Nuclear receptor DAX-1, Nuclear receptor subfamily 0 group B member 1, Nuclear receptor subfamily 3 group C member 4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), OK/SW-cl.56, OTTHUMP00000015956, OTTHUMP00000015964, OTTHUMP00000039393, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, Oncogene GLI3, Oncogene JUN, P150, P53_HUMAN, PAP A, PAPB, PARP cleavage protease, PPD IV, Phosphoprotein p53, Procaspase3, Protein Yama, Proto oncogene tyrosine protein kinase ABL1, Proto-oncogene c-Abl, Proto-oncogene c-jun, QccE-11995, QccE-15186, RP24-311F12.2, RP3 369A17.3, RSTS, RTS, Rubinstein Taybi syndrome, SBMA, SCA-1, SCAN1, SMAX1, SREBP cleavage activity 1, SRXY2, Spinal and bulbar muscular atrophy, Spinal and bulbar muscular atrophy (SBMA), TBA1A_HUMAN, TBA4A_HUMAN, TBB1_HUMAN, TBB2A_HUMAN, TBB2B_HUMAN, TBB3_HUMAN, TBB4B_HUMAN, TBB4_HUMAN, TBB5_HUMAN, TFM, TRP53, TUBA1, TUBA1A, TUBA3, TUBA4A, TUBB, TUBB 1, TUBB 2, TUBB 2A, TUBB 2C, TUBB 4, TUBB PARALOG, TUBB-5, TUBB2B, TUBB4A, TYDP, TYDP1_HUMAN, Testicular Feminization (TFM), Testis-specific alpha-tubulin, Tp53, Transcription factor AP-1, Transcriptional activator GLI3, Transcriptional repressor GLI3R, Transformation related protein 53, Tubb 3, Tubb4b, Tubulin 5 beta, Tubulin B-alpha-1, Tubulin H2-alpha, Tubulin alpha, Tubulin alpha 1a, Tubulin alpha 3, Tubulin alpha brain specific, Tubulin alpha-1 chain, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-4A chain, Tubulin beta 1 class VI, Tubulin beta 2, Tubulin beta 2A, Tubulin beta 2A class IIa, Tubulin beta 2B, Tubulin beta 2B class IIb, Tubulin beta 2C, Tubulin beta 3, Tubulin beta 4, Tubulin beta 4A class IVa, Tubulin beta 5, Tubulin beta IV, Tubulin beta chain, Tubulin beta class I, Tubulin beta polypeptide, Tubulin beta polypeptide 2, Tubulin beta polypeptide paralog, Tubulin beta-1 chain, Tubulin beta-2 chain, Tubulin beta-2A chain, Tubulin beta-2B chain, Tubulin beta-2C chain, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-4B chain, Tubulin beta-5 chain, Tubulin beta-III, Tubulin, alpha 1 (testis specific), Tubulin, alpha 4a, Tubulin, alpha, testis-specific, Tubulin, alpha-1, Tubulin, beta 1, Tubulin, beta, class IVB, Tubulin, beta-4B, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, Tyr-DNA phosphodiesterase 1, Tyrosine-protein kinase ABL1, Tyrosyl-DNA phosphodiesterase 1, V jun sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog (avian), V-jun avian sarcoma virus 17 oncogene homolog, Yama, Yama protein, Zinc finger protein GLI 3, androgen receptor splice variant 4b, bA408E5.3, bA506K6.1, bcr/abl, beta 1 tubulin, beta 2 tubulin, beta 3 tubulin, beta 5, beta Ib tubulin, beta four tubulin, beta-4, c ABL, c abl oncogene 1 non receptor tyrosine kinase, c abl oncogene 1 receptor tyrosine kinase, cJun, class IIa beta-tubulin, class IIb beta-tubulin, dJ40E16.7, dJ543J19.4, erythroid derived 2, like 2, mldy, nuclear factor erythroid 2 like 2, p39, p53 tumor suppressor, tuba1l, tubulin, alpha 1, like, tubulin, beta 4B class IVb, tuj 1, tumor antigen p55, v abl, v abl Abelson murine leukemia viral oncogene homolog 1

2 Images
Immunoprecipitation - Nocodazole, Microtubule inhibitor (AB120630)
  • IP

Lab

Immunoprecipitation - Nocodazole, Microtubule inhibitor (AB120630)

Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.

Lane 1 : HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
Lane 2 : ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

true

Exposure time: 1s

Chemical Structure - Nocodazole, Microtubule inhibitor (AB120630)
  • Chemical Structure

Lab

Chemical Structure - Nocodazole, Microtubule inhibitor (AB120630)

2D chemical structure image of ab120630, Nocodazole, Microtubule inhibitor

Key facts

CAS number

31430-18-9

Purity

>98%

Form

Solid

form

Molecular weight

301.32 Da

Molecular formula

C<sub>1</sub><sub>4</sub>H<sub>1</sub><sub>1</sub>N<sub>3</sub>O<sub>3</sub>S

PubChem

4122

Nature

Synthetic

Solubility

Soluble in DMSO to 50 mM

Biochemical name

Nocodazole

Biological description

Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.

Canonical smiles

COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CS3

InChi

InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)

InChiKey

KYRVNWMVYQXFEU-UHFFFAOYSA-N

IUPAC Name

methyl N-[6-(thiophene-2-carbonyl)-1H-benzimidazol-2-yl]carbamate

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
The product can be stored for up to 12 months

Product protocols

Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 12:1490781 PubMed39610707

2024

Chromosome alignment and Kif18A action rely on spindle-localized control of Cdk1 activity.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Flavia Serpico,Caterina Pisauro,Asia Trano,Domenico Grieco

BMC biology 22:101 PubMed38685010

2024

Modulation of the microhomology-mediated end joining pathway suppresses large deletions and enhances homology-directed repair following CRISPR-Cas9-induced DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Baolei Yuan,Chongwei Bi,Yeteng Tian,Jincheng Wang,Yiqing Jin,Khaled Alsayegh,Muhammad Tehseen,Gang Yi,Xuan Zhou,Yanjiao Shao,Fernanda Vargas Romero,Wolfgang Fischle,Juan Carlos Izpisua Belmonte,Samir Hamdan,Yanyi Huang,Mo Li

ACS pharmacology & translational science 7:667-679 PubMed38481685

2024

Investigating LIM (Lin-11, Isl-1, and Mec-3) Kinases and Their Correlation with Pathological Events and Microtubule Dynamics in an Experimental Model of Spinal Cord Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Roy,Zarna Pathak,Hemant Kumar

Journal of neurochemistry 168:100-114 PubMed38102893

2023

Mechanisms of aquaporin-4 vesicular trafficking in mammalian cells.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Markou,Philip Kitchen,Ahmed Aldabbagh,Mariaelena Repici,Mootaz M Salman,Roslyn M Bill,Zita Balklava

Biophysical journal 122:1198-1210 PubMed36772795

2023

Differential contractility regulates cancer stem cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Rachel K Heussner,Hongrong Zhang,Guhan Qian,Mikayla J Baker,Paolo P Provenzano

Cell proliferation 55:e13240 PubMed35509151

2022

CDK5RAP3, an essential regulator of checkpoint, interacts with RPL26 and maintains the stability of cell growth.

Applications

Unspecified application

Species

Unspecified reactive species

Hongchen Yan,Jun-Jie Xu,Ilyas Ali,Wei Zhang,Ming Jiang,Guiping Li,Yong Teng,Guangxun Zhu,Yafei Cai

International journal of oncology 60: PubMed35419613

2022

Genome‑wide DNA hypomethylation drives a more invasive pancreatic cancer phenotype and has predictive occult distant metastasis and prognosis potential.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhei Endo,Koichi Suzuki,Yasuaki Kimura,Sawako Tamaki,Hidetoshi Aizawa,Iku Abe,Fumiaki Watanabe,Takaharu Kato,Masaaki Saito,Kazushige Futsuhara,Hiroshi Noda,Fumio Konishi,Toshiki Rikiyama

Cell metabolism 32:967-980.e5 PubMed33264602

2020

Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bowen Wu,Jingtao Qiu,Tuantuan V Zhao,Yanan Wang,Toshihisa Maeda,Isabel N Goronzy,Mitsuhiro Akiyama,Shozo Ohtsuki,Ke Jin,Lu Tian,Jörg J Goronzy,Cornelia M Weyand

International journal of molecular sciences 21: PubMed32033476

2020

A Role of Microtubules in Oligodendrocyte Differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yoon Lee,Eun-Mi Hur

International journal of oncology 54:2222-2236 PubMed30942440

2019

Tumor‑suppressive microRNA‑223 targets WDR62 directly in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Sugita,Hirofumi Yoshino,Masaya Yonemori,Kazutaka Miyamoto,Ryosuke Matsushita,Takashi Sakaguchi,Toshihiko Itesako,Shuichi Tatarano,Masayuki Nakagawa,Hideki Enokida
View all publications

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