MW 301.32 Da, Purity >98%. Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.
2810484G07Rik, A830040C14Rik, ABL, ABL proto oncogene 1 non receptor tyrosine kinase, ABL1_HUMAN, ACLS, AHC, AHCH, AHX, AI838772, AIS, ALS22, ANDR_HUMAN, AP-1, APOPAIN, AR, AR8, AW493413, Abelson murine leukemia viral oncogene homolog 1, Abelson tyrosine protein kinase 1, Activator protein 1, Alpha-tubulin 1, Alpha-tubulin 3, Androgen nuclear receptor variant 2, Androgen receptor, Androgen receptor (dihydrotestosterone receptor; testicular feminization; spinal and bulbar muscular atrophy; Kennedy disease), Antigen NY-CO-13, Apoptotic protease Mch-3, B ALPHA 1, BCC7, Bcr/c abl oncogene protein, Beta 4 tubulin, Beta 5 tubulin, Beta tubulin 1, class VI, Beta-tubulin, Beta2, CASP3_HUMAN, CASP7_HUMAN, CBP_HUMAN, CC3, CDCBM, CDCBM1, CFEOM3, CFEOM3A, CMH-1, CPP-32, CPP32B, CREB-binding protein, Casp3a, Caspase 3, apoptosis-related cysteine peptidase, Caspase 3, apoptosis-related cysteine protease, Caspase 3, apoptosis-related cysteine protease a, Caspase 7, Caspase 7 apoptosis related cysteine peptidase, Caspase-3, Caspase-3 subunit p12, Caspase-7 subunit p11, Cellular tumor antigen p53, Class I beta tubulin, Class II beta tubulin isotype, Class IVb beta tubulin, Class VI beta tubulin, Cyclic AMP responsive enhancer binding protein, Cysteine protease CPP32, DAX 1, DHTR, DKFZp566F223, DNA binding protein, DNA replication inhibitor, DSS-AHC critical region on the X chromosome protein 1, Dihydro testosterone receptor, Dihydrotestosterone receptor (DHTR), Dosage sensitive sex reversal, Dystonia 4 torsion (autosomal dominant), EC 3.4.22.56, Enhancer Binding Protein AP1, FEOM3, FLJ11090, FLJ25113, FLJ92943, FLJ98847, GCPS, GEMI_HUMAN, GLI Kruppel family member GLI 3, GLI Kruppel family member GLI3 (Greig cephalopolysyndactyly syndrome), GLI family zinc finger 3, GLI3 C-terminally truncated form, GLI3 form of 190 kDa, GLI3 form of 83 kDa, GLI3 full length protein, GLI3-190, GLI3-83, GLI3FL, GLI3_HUMAN, GMNN, GTD, Geminin, Geminin DNA replication inhibitor, Glioma associated oncogene family zinc finger 3, H2 ALPHA, HHG, HUMARA, HYSP1, Hum a tub1, Hum a tub2, ICE-LAP3, ICE-like apoptotic protease 3, ICE3, JTK7, JUN protein, JUNC, JUN_HUMAN, Jun Activation Domain Binding Protein, Jun oncogene, Jun proto oncogene, KAT3A, KD, Kennedy disease (KD), LFS1, LICE, LICE2, LIS3, M(beta)1, M(beta)2, M(beta)3, M(beta)6, M40, MC1-R, MCH3, MGC104252, MGC112732, MGC117247, MGC16435, MGC171407, MGC55332, MGC8685, Mutant tumor protein 53, NF-E2-related factor 2, NF2L2_HUMAN, NR0B1_HUMAN, NR3C4, NRF2, NROB1, Neuron specific beta III Tubulin, Neuron-specific class III beta-tubulin, Nfe2l2, Nuclear factor, Nuclear factor (erythroid derived 2) like 2, Nuclear factor erythroid 2-related factor 2, Nuclear factor erythroid derived 2 like 2, Nuclear hormone receptor, Nuclear receptor 0B1, Nuclear receptor DAX-1, Nuclear receptor subfamily 0 group B member 1, Nuclear receptor subfamily 3 group C member 4, Nuclear receptor subfamily 3 group C member 4 (NR3C4), OK/SW-cl.56, OTTHUMP00000015956, OTTHUMP00000015964, OTTHUMP00000039393, OTTHUMP00000165052, OTTHUMP00000165053, OTTHUMP00000165054, Oncogene GLI3, Oncogene JUN, P150, P53_HUMAN, PAP A, PAPB, PARP cleavage protease, PPD IV, Phosphoprotein p53, Procaspase3, Protein Yama, Proto oncogene tyrosine protein kinase ABL1, Proto-oncogene c-Abl, Proto-oncogene c-jun, QccE-11995, QccE-15186, RP24-311F12.2, RP3 369A17.3, RSTS, RTS, Rubinstein Taybi syndrome, SBMA, SCA-1, SCAN1, SMAX1, SREBP cleavage activity 1, SRXY2, Spinal and bulbar muscular atrophy, Spinal and bulbar muscular atrophy (SBMA), TBA1A_HUMAN, TBA4A_HUMAN, TBB1_HUMAN, TBB2A_HUMAN, TBB2B_HUMAN, TBB3_HUMAN, TBB4B_HUMAN, TBB4_HUMAN, TBB5_HUMAN, TFM, TRP53, TUBA1, TUBA1A, TUBA3, TUBA4A, TUBB, TUBB 1, TUBB 2, TUBB 2A, TUBB 2C, TUBB 4, TUBB PARALOG, TUBB-5, TUBB2B, TUBB4A, TYDP, TYDP1_HUMAN, Testicular Feminization (TFM), Testis-specific alpha-tubulin, Tp53, Transcription factor AP-1, Transcriptional activator GLI3, Transcriptional repressor GLI3R, Transformation related protein 53, Tubb 3, Tubb4b, Tubulin 5 beta, Tubulin B-alpha-1, Tubulin H2-alpha, Tubulin alpha, Tubulin alpha 1a, Tubulin alpha 3, Tubulin alpha brain specific, Tubulin alpha-1 chain, Tubulin alpha-1A chain, Tubulin alpha-1B chain, Tubulin alpha-3 chain, Tubulin alpha-4A chain, Tubulin beta 1 class VI, Tubulin beta 2, Tubulin beta 2A, Tubulin beta 2A class IIa, Tubulin beta 2B, Tubulin beta 2B class IIb, Tubulin beta 2C, Tubulin beta 3, Tubulin beta 4, Tubulin beta 4A class IVa, Tubulin beta 5, Tubulin beta IV, Tubulin beta chain, Tubulin beta class I, Tubulin beta polypeptide, Tubulin beta polypeptide 2, Tubulin beta polypeptide paralog, Tubulin beta-1 chain, Tubulin beta-2 chain, Tubulin beta-2A chain, Tubulin beta-2B chain, Tubulin beta-2C chain, Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-4B chain, Tubulin beta-5 chain, Tubulin beta-III, Tubulin, alpha 1 (testis specific), Tubulin, alpha 4a, Tubulin, alpha, testis-specific, Tubulin, alpha-1, Tubulin, beta 1, Tubulin, beta, class IVB, Tubulin, beta-4B, Tumor protein 53, Tumor protein p53, Tumor suppressor p53, Tyr-DNA phosphodiesterase 1, Tyrosine-protein kinase ABL1, Tyrosyl-DNA phosphodiesterase 1, V jun sarcoma virus 17 oncogene homolog, V jun sarcoma virus 17 oncogene homolog (avian), V-jun avian sarcoma virus 17 oncogene homolog, Yama, Yama protein, Zinc finger protein GLI 3, androgen receptor splice variant 4b, bA408E5.3, bA506K6.1, bcr/abl, beta 1 tubulin, beta 2 tubulin, beta 3 tubulin, beta 5, beta Ib tubulin, beta four tubulin, beta-4, c ABL, c abl oncogene 1 non receptor tyrosine kinase, c abl oncogene 1 receptor tyrosine kinase, cJun, class IIa beta-tubulin, class IIb beta-tubulin, dJ40E16.7, dJ543J19.4, erythroid derived 2, like 2, mldy, nuclear factor erythroid 2 like 2, p39, p53 tumor suppressor, tuba1l, tubulin, alpha 1, like, tubulin, beta 4B class IVb, tuj 1, tumor antigen p55, v abl, v abl Abelson murine leukemia viral oncogene homolog 1
MW 301.32 Da, Purity >98%. Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.
Soluble in DMSO to 50 mM.
Microtubule inhibitor. Rapidly depolymerises microtubules in vivo and inhibits tubulin polymerisation in vitro. Inhibits mitosis. Also reduces stem cell pluripotency factors Oct 4 and Nanog.
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2D chemical structure image of ab120630, Nocodazole, Microtubule inhibitor
Vimentin (phospho S56) was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate with Anti-Vimentin (phospho S56) antibody [EPR21084] ab217673 at 1/30 dilution. Western blot was performed from the immunoprecipitate using Anti-Vimentin (phospho S56) antibody [EPR21084] ab217673 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution.
Lane 1: HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate 10 μg (Input).
Lane 2: Anti-Vimentin (phospho S56) antibody [EPR21084] ab217673 IP in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-Vimentin (phospho S56) antibody [EPR21084] ab217673 in HeLa treated with 100 ng/ml nocodazole (ab120630) for 18 hours, whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Developed using the ECL technique.
Exposure time: 1s
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