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AB120295

NS 398, COX-2 inhibitor

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(5 Publications)

MW 314.36 Da, Purity >98%. Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.

View Alternative Names

Cyclooxygenase, Cyclooxygenase 2b, Cyclooxygenase 3, included, Cyclooxygenase-1, Cyclooxygenase-2, EC 1.14.99.1, GRIPGHS, Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase, Glucocorticoid-regulated inflammatory cyclooxygenase, Macrophage activation-associated marker protein P71/73, OTTHUMP00000033524, PCOX1, PES-2, PGG/HS, PGH synthase 1, PGH synthase 2, PGH1_HUMAN, PGH2_HUMAN, PGHS-1, PGHS-2, PHS 1, PHS 2, PHS II, PTGHS, PTGS1, PTGS2, Partial COX1 proteins, included, Prostaglandin G/H synthase, Prostaglandin G/H synthase 1, Prostaglandin G/H synthase 2, Prostaglandin G/H synthase 2 precursor, Prostaglandin G/H synthase and cyclooxygenase, Prostaglandin H2 synthase 1, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 1, Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase), Prostaglandin-endoperoxide synthase 2, TIS10, TIS10 protein, fj02a10, hCox 2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), ptgs2a, unp1239, wu:fj02a10

2 Images
Immunocytochemistry/ Immunofluorescence - NS 398, COX-2 inhibitor (AB120295)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - NS 398, COX-2 inhibitor (AB120295)

ab32034 staining p27 KIP1 in MCF7 cells treated with NS 398 (ab120295), by ICC/IF. Increase in p27 KIP1 expression correlates with increased concentration of NS 398, as described in literature.
The cells were incubated at 37°C for 6h in media containing different concentrations of ab120295 (NS 398) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32034 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Chemical Structure - NS 398, COX-2 inhibitor (AB120295)
  • Chemical Structure

Lab

Chemical Structure - NS 398, COX-2 inhibitor (AB120295)

2D chemical structure image of ab120295, NS 398, COX-2 inhibitor

Key facts

CAS number

123653-11-2

Purity

>98%

Form

Solid

form

Molecular weight

314.36 Da

Molecular formula

C<sub>1</sub><sub>3</sub>H<sub>1</sub><sub>8</sub>N<sub>2</sub>O<sub>5</sub>S

PubChem

4553

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

N-(2-Cyclohexyloxy-4-nitrophenyl)methanesulfonamide

Biological description

Selective cyclooxygenase-2 inhibitor (IC50 values are 3.8 and >100 μM for COX-2 and COX-1, respectively). Orally active. Anti-inflammatory, antipyretic, analgesic and non-ulcerogenic in vivo. Induces apoptosis and cell cycle arrest.

Canonical smiles

CS(=O)(=O)NC1=C(C=C(C=C1)[N+](=O)[O-])OC2CCCCC2

InChi

InChI=1S/C13H18N2O5S/c1-21(18,19)14-12-8-7-10(15(16)17)9-13(12)20-11-5-3-2-4-6-11/h7-9,11,14H,2-6H2,1H3

InChiKey

KTDZCOWXCWUPEO-UHFFFAOYSA-N

IUPAC Name

N-(2-cyclohexyloxy-4-nitrophenyl)methanesulfonamide

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Cyclooxygenase 2 (COX2) and Cyclooxygenase 1 (COX1) are enzymes that convert arachidonic acid into prostaglandins which are involved in inflammation and other physiological functions. COX2 also known as prostaglandin-endoperoxide synthase 2 has a molecular mass of approximately 72 kDa. COX1 sometimes called prostaglandin-endoperoxide synthase 1 has similar molecular weight. These enzymes are expressed in various tissues with COX1 found ubiquitously and COX2 expressed more selectively often induced by inflammatory stimuli.
Biological function summary

COX2 and COX1 help regulate processes like inflammation pain and fever through their role in prostaglandin synthesis. COX1 primarily maintains normal cellular processes while COX2 is highly inducible and becomes active during inflammatory responses. COX2 does not typically form complexes operating independently in the cellular environment to mediate inflammatory signals.

Pathways

COX2 and COX1 are key components in the prostaglandin biosynthetic pathway influencing pain and inflammatory pathways. Their activity affects the cyclooxygenase pathway important for converting arachidonic acid into prostaglandins and thromboxanes. COX enzymes interact closely with other proteins like prostaglandin synthases and thromboxane synthase which further modify their products into different prostaglandins and thromboxanes.

COX2 links to conditions such as rheumatoid arthritis and colorectal cancer. Its increased expression often correlates with the inflammatory response seen in these illnesses. Inhibiting COX2 with drugs like lumiracoxib alleviates symptoms in inflammatory diseases. COX1 while less implicated in disease connects to interactions with other proteins indicating changes during digestive issues due to its role in gastric mucosa maintenance.
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Product protocols

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 98:e0030024 PubMed39382324

2024

Retinoic acid-induced differentiation and oxidative stress inhibitors increase resistance of human neuroblastoma cells to La Crosse virus-induced cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Paul F Policastro,Christine A Schneider,Clayton W Winkler,Jacqueline M Leung,Karin E Peterson

Journal of clinical biochemistry and nutrition 73:145-153 PubMed37700846

2023

NEK7 inhibition attenuates Aβ-induced cognitive impairment by regulating TLR4/NF-κB and the NLRP3 inflammasome in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Li,Yifan He,Qian Yang,Hena Guo,Nini Li,Dongdong Zhang

Frontiers in cellular neuroscience 15:772549 PubMed34887729

2021

Astroglial CB1 Cannabinoid Receptors Mediate CP 55,940-Induced Conditioned Place Aversion Through Cyclooxygenase-2 Signaling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Cong,Kangrong Lu,Wenjie Zou,Ziming Li,Zhipeng Guo,Xiangzhen Tong,Jiawei Zheng,Jianping Zhu,Shuji Li,Wangming Zhang,Yanwu Guo,Tian-Ming Gao,Rongqing Chen

PLoS pathogens 17:e1009927 PubMed34516571

2021

Host phospholipid peroxidation fuels ExoU-dependent cell necrosis and supports Pseudomonas aeruginosa-driven pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Salimata Bagayoko,Stephen Adonai Leon-Icaza,Miriam Pinilla,Audrey Hessel,Karin Santoni,David Péricat,Pierre-Jean Bordignon,Flavie Moreau,Elif Eren,Aurélien Boyancé,Emmanuelle Naser,Lise Lefèvre,Céline Berrone,Nino Iakobachvili,Arnaud Metais,Yoann Rombouts,Geanncarlo Lugo-Villarino,Agnès Coste,Ina Attrée,Dara W Frank,Hans Clevers,Peter J Peters,Céline Cougoule,Rémi Planès,Etienne Meunier

Genes to cells : devoted to molecular & cellular m 25:197-214 PubMed31989743

2020

Prostaglandin E and its receptor EP2 trigger signaling that contributes to YAP-mediated cell competition.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Ishihara,Yuya Nagaoka,Toshiaki Okuno,Satoshi Kofuji,Mari Ishigami-Yuasa,Hiroyuki Kagechika,Kenya Kamimura,Shuji Terai,Takehiko Yokomizo,Yukihiko Sugimoto,Yasuyuki Fujita,Akira Suzuki,Hiroshi Nishina
View all publications
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