MW 392.5 Da. Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.9 and 4.4 μM for inhibition of dynamin I and II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated endocytosis (IC50 = 6.7 μM). Inhibits cancer cell growth.
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- International journal of molecular sciences 24:2023ATP Consumption Is Coupled with Endocytosis in Exudated Neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuo Wang,Zirui Zeng,Mengyue Shen,Ryuji Okazaki,Hironori Miyata,Tomo Yonezawa,Yasuhiro YoshidaPubMed 37240384
- International immunopharmacology 84:1065102020ERK is involved in the differentiation and function of dimethyl sulfoxide-induced HL-60 neutrophil-like cells, which mimic inflammatory neutrophils.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDuo Wang et. al.PubMed 32361568
- Trends in cell biology 23:90-1012012Targeting membrane trafficking in infection prophylaxis: dynamin inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCallista B Harper,Michel R Popoff,Adam McCluskey,Phillip J Robinson,Frédéric A MeunierPubMed 23164733
- Molecular cancer therapeutics 9:1995-20062010The dynamin inhibitors MiTMAB and OcTMAB induce cytokinesis failure and inhibit cell proliferation in human cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanket Joshi et. al.PubMed 20571068
- Molecular pharmacology 72:1425-392007Myristyl trimethyl ammonium bromide and octadecyl trimethyl ammonium bromide are surface-active small molecule dynamin inhibitors that block endocytosis mediated by dynamin I or dynamin II.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnie Quan et. al.PubMed 17702890
Key facts
- CAS number
1120-02-1
- Form
Solid
- Molecular weight
392.5 Da
- Molecular formula
C21H46BrN
- PubChem identifier
70708
- Nature
Synthetic
Alternative names
B dynamin, DNM, DNM 1, DYN1_HUMAN, Dynamin, Dynamin-1
Recommended products
MW 392.5 Da. Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.9 and 4.4 μM for inhibition of dynamin I and II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated endocytosis (IC50 = 6.7 μM). Inhibits cancer cell growth.
Alternative names
B dynamin, DNM, DNM 1, DYN1_HUMAN, Dynamin, Dynamin-1
Key facts
- CAS number
1120-02-1
- Form
Solid
- Molecular weight
392.5 Da
- Molecular formula
C21H46BrN
- PubChem identifier
70708
- Nature
Synthetic
- Solubility
Soluble in DMSO to 25 mM (with warming).
- Biochemical name
Trimethyloctadecylammonium bromide
- Biological description
Cell-permeable dynamin I and dynamin II inhibitor (IC50 values are 1.9 and 4.4 μM for inhibition of dynamin I and II GTPase, respectively). Targets the pleckstrin homology (PH) (lipid binding) domain. Competitive with phospholipid and non-competitive with GTP. Inhibits receptor-mediated endocytosis (IC50 = 6.7 μM). Inhibits cancer cell growth.
- Canonical SMILES
CCCCCCCCCCCCCCCCCC[N+](C)(C)C.[Br-]
- InChI
InChI=1S/C21H46N.BrH/c1-5-6-7-8-9-10-11-12-13-14-15-16-17-18-19-20-21-22(2,3)4;/h5-21H2,1-4H3;1H/q+1;/p-1
- InChIKey
SZEMGTQCPRNXEG-UHFFFAOYSA-M
- IUPAC name
trimethyl(octadecyl)azanium;bromide
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
- Storage information
Store under desiccating conditions, The product can be stored for up to 12 months
Notes
Sold under exclusive licence from Children's Medical Research Institute and Newcastle Innovation Ltd. OcTMAB™ is a trademark of Children's Medical Research Institute and Newcastle Innovation Ltd.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dynamin 1 also known as DNM1 is a GTPase with a molecular mass of approximately 96 kDa. It plays a mechanical role in membrane fission during endocytosis. Dynamin 1 is primarily expressed in neuronal tissues where it participates in the formation and release of synaptic vesicles. Its ability to hydrolyze GTP gives it the mechanical force needed to constrict and sever membranes which is vital for efficient synaptic vesicle recycling in neurons.
- Biological function summary
Dynamin 1 helps regulate synaptic transmission by controlling vesicular traffic in neurons. As part of a larger protein complex it interacts with various proteins to mediate endocytosis specifically in presynaptic nerve terminals. These interactions ensure that synaptic vesicles are recycled rapidly allowing neurons to maintain communication across synapses. Disruptions in dynamin 1 function can impair synaptic vesicle recycling affecting neurotransmission efficiency.
- Pathways
Dynamin 1 is essential in the clathrin-mediated endocytic pathway. This pathway is a major route for internalizing synaptic vesicles and involves cooperation with other proteins such as clathrin and adaptor proteins. Additionally dynamin 1 participates in the actin cytoskeleton dynamics linking it with actin-associated proteins. These pathways highlight its contributions to maintaining synaptic vesicle endocytosis and neurite outgrowth processes.
- Associated diseases and disorders
Alterations in dynamin 1 function are linked to neurological disorders including epilepsy and Huntington's disease. In epilepsy improper functioning of dynamin 1 disrupts synaptic vesicle recycling leading to imbalances in neurotransmitter release. Connections exist between dynamin 1 and huntingtin where their interactions in vesicular trafficking are important for neuronal stability. Malfunctions in these processes often result in neurodegenerative symptoms emphasizing dynamin 1's involvement in sustaining neural health.
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2 product images
Chemical Structure - OcTMAB™, dynamin I and dynamin II inhibitor (ab120467)
2D chemical structure image of ab120467, OcTMAB™, dynamin I and dynamin II inhibitor
Immunocytochemistry/ Immunofluorescence - OcTMAB™, dynamin I and dynamin II inhibitor (ab120467)
Anti-PAI1 antibody ab66705 staining PAI1 in HeLa cells treated with OcTMAB™ (ab120467), by ICC/IF. Increase in PAI1 expression correlates with increased concentration of OcTMAB™, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120467 (OcTMAB™) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-PAI1 antibody ab66705 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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