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AB120234

PD 98059, MEK1 inhibitor

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(23 Publications)

MW 267.28 Da, Purity >99%. Inhibitor of MKK1 (MEK1). Achieve your results faster with highly validated, pure and trusted compounds.
2 Images
Immunocytochemistry/ Immunofluorescence - PD 98059, MEK1 inhibitor (AB120234)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - PD 98059, MEK1 inhibitor (AB120234)

ab18195 staining hnRNP K in HeLa cells treated with PD 98059 (ab120234), by ICC/IF. Changes in localization of hnRNP K (translocation from cytoplasm to nucleous) correlates with increased concentration of hnRNP K, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120234 ( hnRNP K) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab18195 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Chemical Structure - PD 98059, MEK1 inhibitor (AB120234)
  • Chemical Structure

Lab

Chemical Structure - PD 98059, MEK1 inhibitor (AB120234)

2D chemical structure image of ab120234, PD 98059, MEK1 inhibitor

Key facts

CAS number

167869-21-8

Purity

>99%

Form

Solid

form

Molecular weight

267.28 Da

Molecular formula

C<sub>1</sub><sub>6</sub>H<sub>1</sub><sub>3</sub>NO<sub>3</sub>

PubChem

4713

Nature

Synthetic

Solubility

Soluble in DMSO to 25 mM

Soluble in ethanol to 5 mM

Soluble in DMSO to 25 mM and in ethanol to 5 mM

Biochemical name

2-(2-Amino-3-methoxyphenyl)-4H-1-benzopyran-4-one

Biological description

Inhibitor of MKK1 (MEK1).

Canonical smiles

COC1=CC=CC(=C1N)C2=CC(=O)C3=CC=CC=C3O2

InChi

InChI=1S/C16H13NO3/c1-19-14-8-4-6-11(16(14)17)15-9-12(18)10-5-2-3-7-13(10)20-15/h2-9H,17H2,1H3

InChiKey

QFWCYNPOPKQOKV-UHFFFAOYSA-N

IUPAC Name

2-(2-amino-3-methoxyphenyl)chromen-4-one

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Complementary Products

Biochemicals

AB120003

D-AP5, NMDA glutamate site antagonist

Chemical Structure - D-AP5, NMDA glutamate site antagonist (AB120003)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Biochemicals

AB146592

PD 98059 (DMSO solution), MEK1 inhibitor

Chemical Structure - PD 98059 (DMSO solution), MEK1 inhibitor (AB146592)

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Biochemicals

AB120243

LY 294002, PI3-kinase inhibitor

Western blot - LY 294002, PI3-kinase inhibitor (AB120243)

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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient
Storage information
The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MEK1 also known as MAP2K1 is a dual-specificity kinase that plays a role in the MAPK/ERK pathway. It has a molecular weight of around 45 kDa. MEK1 phosphorylates and activates the extracellular signal-regulated kinases ERK1 and ERK2. MEK1 is expressed in various tissues but is especially abundant in brain and heart tissues. Researchers commonly measure MEK1 levels using techniques like MEK1 ELISA while phospho-MEK antibodies help analyze its activity state.
Biological function summary

MEK1 impacts cell processes such as proliferation differentiation and survival. It does not work alone but forms a complex with other proteins to exert its function. MEK1 activity is tightly controlled by upstream activators and downstream targets. One of the key phosphorylation sites on MEK1 is at serine 298 often marked as MEK1 pS298. This phosphorylation indicates MEK1's activated state which is critical for its biological function.

Pathways

MEK1 integrates signals within the MAPK/ERK pathway and interacts closely with RAF kinases upstream and ERK kinases downstream. This pathway modulates cellular responses to growth factors. MEK1 undergoes phosphorylation enhancing its activity and subsequently phosphorylates ERK proteins. Many studies isolate MEK1 activity using specific inhibitors such as the MEK1 inhibitor to dissect pathway dynamics. ERK inhibitor PD98059 also helps when studying parallel interactions with MEK1.

MEK1 mutations or dysregulation have links to several types of cancer including melanoma. These often involve changes that lead to continuous MEK1 activation contributing to uncontrolled cell growth. MEK1 involvement in the RASopathies also shows its connection to disorders characterized by heart and facial abnormalities. In cancer MEK1 works closely with the BRAF protein which often harbors mutations leading to disease progression showcasing MEK1 as a target for therapeutic intervention.
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Product protocols

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Translational psychiatry 12:522 PubMed36550125

2022

ERK/mTOR signaling may underlying the antidepressant actions of rapastinel in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mengxin Shen,Dan Lv,Xinya Liu,Chuang Wang

Frontiers in immunology 12:755862 PubMed34867992

2021

Co-Exposure of Cardiomyocytes to IFN-γ and TNF-α Induces Mitochondrial Dysfunction and Nitro-Oxidative Stress: Implications for the Pathogenesis of Chronic Chagas Disease Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

João Paulo Silva Nunes,Pauline Andrieux,Pauline Brochet,Rafael Ribeiro Almeida,Eduardo Kitano,André Kenji Honda,Leo Kei Iwai,Débora Andrade-Silva,David Goudenège,Karla Deysiree Alcântara Silva,Raquel de Souza Vieira,Débora Levy,Sergio Paulo Bydlowski,Frédéric Gallardo,Magali Torres,Edimar Alcides Bocchi,Miguel Mano,Ronaldo Honorato Barros Santos,Fernando Bacal,Pablo Pomerantzeff,Francisco Rafael Martins Laurindo,Priscila Camillo Teixeira,Helder I Nakaya,Jorge Kalil,Vincent Procaccio,Christophe Chevillard,Edecio Cunha-Neto

Journal of neuroinflammation 18:150 PubMed34225736

2021

Red nucleus IL-33 facilitates the early development of mononeuropathic pain in male rats by inducing TNF-α through activating ERK, p38 MAPK, and JAK2/STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Hao-Nan Li,Qing-Qing Yang,Wen-Tao Wang,Xue Tian,Fan Feng,Shu-Ting Zhang,Yu-Tong Xia,Jia-Xue Wang,Yuan-Wu Zou,Jun-Yang Wang,Xiao-Yan Zeng

Glia 69:1478-1493 PubMed33556209

2021

Expression and secretion of apoE isoforms in astrocytes and microglia during inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Fe Lanfranco,Jordy Sepulveda,Gregory Kopetsky,G William Rebeck

International journal of molecular medicine 47:256-266 PubMed33236148

2020

TGF‑β1‑induced autophagy activates hepatic stellate cells via the ERK and JNK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Na Jiang,Jian Ping,Lieming Xu

Cell reports 29:4144-4158.e7 PubMed31851939

2019

Discrimination of Dormant and Active Hematopoietic Stem Cells by G Marker Reveals Dormancy Regulation by Cytoplasmic Calcium.

Applications

Unspecified application

Species

Unspecified reactive species

Tsuyoshi Fukushima,Yosuke Tanaka,Fiona K Hamey,Chih-Hsiang Chang,Toshihiko Oki,Shuhei Asada,Yasutaka Hayashi,Takeshi Fujino,Taishi Yonezawa,Reina Takeda,Kimihito Cojin Kawabata,Tomofusa Fukuyama,Terumasa Umemoto,Keiyo Takubo,Hitoshi Takizawa,Susumu Goyama,Yasushi Ishihama,Hiroaki Honda,Berthold Göttgens,Toshio Kitamura

Biochimica et biophysica acta. Molecular cell rese 1866:1398-1411 PubMed31150695

2019

Regulation of MAP kinase-mediated endothelial dysfunction in hyperglycemia via arginase I and eNOS dysregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Safoura Mazrouei,Fatemeh Sharifpanah,R William Caldwell,Marcus Franz,Alia Shatanawi,Johanna Muessig,Michael Fritzenwanger,P Christian Schulze,Christian Jung

FASEB journal : official publication of the Federa 33:7301-7314 PubMed30860872

2019

The emerging role of xanthine oxidase inhibition for suppression of breast cancer cell migration and metastasis associated with hypercholesterolemia.

Applications

Unspecified application

Species

Unspecified reactive species

Se-Hyun Oh,Soon-Youn Choi,Hyo-Jung Choi,Hye-Myung Ryu,You-Jin Kim,Hee-Yeon Jung,Jang-Hee Cho,Chan-Duck Kim,Sun-Hee Park,Tae-Hwan Kwon,Yong-Lim Kim

Journal of neuroscience research 96:1847-1861 PubMed30216497

2018

Red nucleus interleukin-1β evokes tactile allodynia through activation of JAK/STAT3 and JNK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Jie Guo,Hao-Nan Li,Cui-Ping Ding,Shui-Ping Han,Jun-Yang Wang

Frontiers in molecular neuroscience 11:287 PubMed30186110

2018

M-Calpain Activation Facilitates Seizure Induced KCC2 Down Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wan,Liang Ren,Lulan Chen,Guoxiang Wang,Xu Liu,Benjamin H Wang,Yun Wang
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