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AB147465

Phorbol 12-myristate 13-acetate (PMA), PKC activator (DMSO solution)

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(4 Publications)

MW 616.8 Da, Purity >99%. Potent nanomolar activator of protein kinase C in vivo and in vitro. Binds to C1 domain of protein kinase C, induces membrane translocation and enzyme activation. Also reported to have actions on non-kinase proteins including chimaerins, RasGRP and Unc-13/Munc-13. Extremely potent mouse skin tumor promoter.

View Alternative Names

14-3-3-zetaisoform, AI098070, Atypical protein kinase C-lambda/iota, C80388, CVID9, D14Ertd420e, DXS1179E, EC 2.7.11.13, EPK 2, KPCA_HUMAN, KPCB_HUMAN, KPCD1_HUMAN, KPCD3_HUMAN, KPCD_HUMAN, KPCE_HUMAN, KPCG_HUMAN, KPCI_HUMAN, KPCL_HUMAN, KPCT_HUMAN, KPCZ_HUMAN, Kinase PKC delta, May-01, MGC 5363, MGC125656, MGC125657, MGC126514, MGC141919, MGC26269, MGC26534, MGC49908, MGC57564, NU, OTTHUMP00000001368, OTTHUMP00000043364, OTTHUMP00000043365, OTTHUMP00000044160, OTTHUMP00000067291, PCKd, PKC, PKC 0, PKC 2, PKC MU, PKC ZETA, PKC d, PKC delta, PKC epsilon, PKC h, PKC lambda, PKC lambda/iota, PKC-A, PKC-B, PKC-L, PKC-alpha, PKC-beta, PKC-gamma, PKCC, PKCE, PKCG, PKCI, PKCM, PKCiota, PKCnu, PKD, PKD 3, PKM-zeta, included, PRK D3, PRKC D, PRKC delta, PRKC iota, PRKC-lambda/iota, PRKCA, PRKCB, PRKCB II, PRKCB1, PRKCB2, PRKCE, PRKCG, PRKCI, PRKCL, PRKCM, PRKCN, PRKCT, PRKCZ, PRKD 1, Pkcea, Pkcz, Prkch, Prkcq, Protein Kinase C alpha, Protein Kinase C delta, Protein kinase C, Protein kinase C alpha type, Protein kinase C beta, Protein kinase C beta type, Protein kinase C delta VIII, Protein kinase C delta type, Protein kinase C epsilon, Protein kinase C epsilon type, Protein kinase C eta, Protein kinase C eta type, Protein kinase C gamma, Protein kinase C gamma polypeptide, Protein kinase C gamma type, Protein kinase C iota, Protein kinase C iota type, Protein kinase C mu, Protein kinase C mu type, Protein kinase C nu, Protein kinase C nu type, Protein kinase C theta, Protein kinase C theta type, Protein kinase C zeta, Protein kinase C zeta form, Protein kinase C zeta type, Protein kinase D, Protein kinase D1, Protein kinase D3, Protein kinase EPK 2, R74924, SCA 14, Serine threonine protein kinase, Serine/threonine-protein kinase D1, Serine/threonine-protein kinase D3, Tyrosine protein kinase PRKCD, aPKC-lambda/iota, aPKCzeta, nPKC-D1, nPKC-delta, nPKC-epsilon, nPKC-eta, nPKC-iota, nPKC-mu, nPKC-nu, nPKC-theta, nPKC-zeta, r14-3-3, zetaPKC

Key facts

CAS number

16561-29-8

Purity

>99%

Form

Liquid

form

Molecular weight

616.8 Da

Molecular formula

C<sub>3</sub><sub>6</sub>H<sub>5</sub><sub>6</sub>O<sub>8</sub>

PubChem

27924

Nature

Synthetic

Biochemical name

Phorbol 12-myristate 13-acetate

Biological description

Potent nanomolar activator of protein kinase C in vivo and in vitro. Binds to C1 domain of protein kinase C, induces membrane translocation and enzyme activation. Also reported to have actions on non-kinase proteins including chimaerins, RasGRP and Unc-13/Munc-13. Extremely potent mouse skin tumor promoter.

Canonical smiles

CCCCCCCCCCCCCC(=O)OC1C(C2(C(C=C(CC3(C2C=C(C3=O)C)O)CO)C4C1(C4(C)C)OC(=O)C)O)C

Isomeric smiles

CCCCCCCCCCCCCC(=O)O[C@@H]1[C@H]([C@]2([C@@H](C=C(C[C@]3([C@H]2C=C(C3=O)C)O)CO)[C@H]4[C@@]1(C4(C)C)OC(=O)C)O)C

InChi

InChI=1S/C36H56O8/c1-7-8-9-10-11-12-13-14-15-16-17-18-29(39)43-32-24(3)35(42)27(30-33(5,6)36(30,32)44-25(4)38)20-26(22-37)21-34(41)28(35)19-23(2)31(34)40/h19-20,24,27-28,30,32,37,41-42H,7-18,21-22H2,1-6H3/t24-,27+,28-,30-,32-,34-,35-,36-/m1/s1

InChiKey

PHEDXBVPIONUQT-RGYGYFBISA-N

IUPAC Name

[(1S,2S,6R,10S,11R,13S,14R,15R)-13-acetyloxy-1,6-dihydroxy-8-(hydroxymethyl)-4,12,12,15-tetramethyl-5-oxo-14-tetracyclo[8.5.0.02,6.011,13]pentadeca-3,8-dienyl] tetradecanoate

Product details

Supplied in DMSO (10mM).

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

F1000Research 13:1049 PubMed40535171

2025

A guide to selecting high-performing antibodies for STING1 (Uniprot ID: Q86WV6) for use in western blot, immunoprecipitation, and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Ruíz Moleón,Charles Alende,Maryam Fotouhi,Riham Ayoubi,Kathleen Southern,Carl Laflamme

Nature protocols 20:1509-1545 PubMed39690206

2024

A consensus platform for antibody characterization.

Applications

Unspecified application

Species

Unspecified reactive species

Riham Ayoubi,Joel Ryan,Sara Gonzalez Bolivar,Charles Alende,Vera Ruiz Moleon,Maryam Fotouhi,Mona Alqazzaz,Kathleen Southern,Walaa Alshafie,Matt R Baker,Alexander R Ball,Danielle Callahan,Jeffery A Cooper,Katherine Crosby,Kevin J Harvey,Douglas W Houston,Ravindran Kumaran,Meghan Rego,Christine Schofield,Hai Wu,Michael S Biddle,Claire M Brown,Richard A Kahn,Anita Bandrowski,Harvinder S Virk,Aled M Edwards,Peter S McPherson,Carl Laflamme

F1000Research 13:77 PubMed39429638

2024

A guide to selecting high-performing antibodies for PLC-gamma-2 for use in Western Blot, immunoprecipitation and immunofluorescence.

Applications

Unspecified application

Species

Unspecified reactive species

Vera Ruíz Moleón,Maryam Fotouhi,Charles Alende,Riham Ayoubi,Logan M Bedford,Kathleen Southern,Timothy I Richardson,Carl Laflamme

Cytotherapy 26:136-144 PubMed38149947

2023

Generation of human ILC3 from allogeneic and autologous CD34 hematopoietic progenitors toward adoptive transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Jolien M R Van der Meer,Ingrid Bulder,Carlijn Kuijk,Marion Kleijer,Myrddin W Verheij,Said Z Omar,Nienke J E Haverkate,Harry Dolstra,Bianca Blom,Mette D Hazenberg,Carlijn Voermans
View all publications

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