MW 174.19 Da, Purity >98%. Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
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- iScience 26:1081382023Scn2a insufficiency alters spontaneous neuronal Ca activity in somatosensory cortex during wakefulness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelody Li,Mohamed Eltabbal,Hoang-Dai Tran,Bernd KuhnPubMed 37876801
- PeerJ 11:e160602023Fluorofenidone alleviates liver fibrosis by inhibiting hepatic stellate cell autophagy the TGF-β1/Smad pathway: implications for liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiongqun Peng,Huixiang Yang,Lijian Tao,Jingni Xiao,Ya Zeng,Yueming Shen,Xueke Yu,Fei Zhu,Jiao QinPubMed 37790613
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 71:2021Dehydroepiandrosterone protects against acetaminophen-induced liver damage in rats by upregulation of Bcl-2 and activation of sirt signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE M Abd-Ella,A F El-Kott,A E El-Kenawy,H S Khalifa,M M Bin-Meferij,A M AlramlawyPubMed 33727425
- Archives of physiology and biochemistry 129:984-9972021Kaempferol attenuates diabetic nephropathy in streptozotocin-induced diabetic rats by a hypoglycaemic effect and concomitant activation of the Nrf-2/Ho-1/antioxidants axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli S AlshehriPubMed 33625930
- PloS one 11:e01631652016Enlargement of the Axial Length and Altered Ultrastructural Features of the Sclera in a Mutant Lumican Transgenic Mouse Model.
- Autophagy 11:403-152015AU4S: a novel synthetic peptide to measure the activity of ATG4 in living cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhong Ni,Yi Gong,Xufang Dai,Wen Ding,Bin Wang,Haiyan Gong,Liyan Qin,Panke Cheng,Song Li,Jiqin Lian,Fengtian HePubMed 25831015
Key facts
- CAS number
- 329-98-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 174.19 Da
- Molecular formula
- C7H7FO2S
- PubChem identifier
- 4784
- Nature
- Synthetic
Recommended products
MW 174.19 Da, Purity >98%. Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
Key facts
- CAS number
- 329-98-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 174.19 Da
- Molecular formula
- C7H7FO2S
- PubChem identifier
- 4784
- Nature
- Synthetic
- Solubility
Insoluble in H2O.
≥28.3 mg/mL in EtOH.
≥17.4 mg/mL in DMSO.
Insoluble in water.
?17.4 mg/mL in DMSO.
?28.3 mg/mL in EtOH.
- Biochemical name
- Phenylmethylsulfonyl fluoride
- Biological description
Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
- Canonical SMILES
- C1=CC=C(C=C1)CS(=O)(=O)F
- InChI
- InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
- InChIKey
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N
- IUPAC name
- phenylmethanesulfonyl fluoride
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Unstable in water
Storage instructions: Hygroscopic, Under inert atmosphere.
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2 product images
Chemical Structure - PMSF, protease inhibitor (ab141032)
2D chemical structure image of ab141032, PMSF, protease inhibitor
Image from Song Y, et al. Plos One, 11(10), e0163165. Fig 5b,; doi: 10.1371/journal.pone.0163165Cellular Activation - PMSF, protease inhibitor (ab141032)
Analysis of protein expression level in the sclera. The scleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor. Lumican and collagen type I were both overexpressed at the protein level in the transgenic sclera. GAPDH was used as a control. Lane 1 to lane 5, transgenic mice. Lane 6 to lane 9, wild-type mice.
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