MW 174.19 Da, Purity >98%. Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
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- iScience 26:1081382023Scn2a insufficiency alters spontaneous neuronal Ca activity in somatosensory cortex during wakefulness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelody Li,Mohamed Eltabbal,Hoang-Dai Tran,Bernd KuhnPubMed 37876801
- PeerJ 11:e160602023Fluorofenidone alleviates liver fibrosis by inhibiting hepatic stellate cell autophagy the TGF-β1/Smad pathway: implications for liver cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiongqun Peng,Huixiang Yang,Lijian Tao,Jingni Xiao,Ya Zeng,Yueming Shen,Xueke Yu,Fei Zhu,Jiao QinPubMed 37790613
- Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 71:2021Dehydroepiandrosterone protects against acetaminophen-induced liver damage in rats by upregulation of Bcl-2 and activation of sirt signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesE M Abd-Ella,A F El-Kott,A E El-Kenawy,H S Khalifa,M M Bin-Meferij,A M AlramlawyPubMed 33727425
- Archives of physiology and biochemistry 129:984-9972021Kaempferol attenuates diabetic nephropathy in streptozotocin-induced diabetic rats by a hypoglycaemic effect and concomitant activation of the Nrf-2/Ho-1/antioxidants axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli S AlshehriPubMed 33625930
- PloS one 11:e01631652016Enlargement of the Axial Length and Altered Ultrastructural Features of the Sclera in a Mutant Lumican Transgenic Mouse Model.
- Autophagy 11:403-152015AU4S: a novel synthetic peptide to measure the activity of ATG4 in living cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenhong Ni,Yi Gong,Xufang Dai,Wen Ding,Bin Wang,Haiyan Gong,Liyan Qin,Panke Cheng,Song Li,Jiqin Lian,Fengtian HePubMed 25831015
- The Journal of pharmacology and experimental therapeutics 283:1138-431998The effect of the enzyme inhibitor phenylmethylsulfonyl fluoride on the pharmacological effect of anandamide in the mouse model of cannabimimetic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesD R Compton,B R MartinPubMed 9399986
- Nuclear medicine and biology 20:991-21993Evaluation of phenylmethanesulfonyl fluoride (PMSF) as a tracer candidate mapping acetylcholinesterase in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT Irie,K Fukushi,M IyoPubMed 8298579
- The Journal of pharmacology and experimental therapeutics 167:98-1041969The action of phenylmethylsulfonyl fluoride on human acetylcholinesterase, chymotyrpsin and trypsin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP Turini,S Kurooka,M Steer,A N Corbascio,T P SingerPubMed 4306345
Key facts
- CAS number
329-98-6
- Purity
> 98%
- Form
Solid
- Molecular weight
174.19 Da
- Molecular formula
C7H7FO2S
- PubChem identifier
4784
- Nature
Synthetic
Recommended products
MW 174.19 Da, Purity >98%. Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
Key facts
- CAS number
329-98-6
- Purity
> 98%
- Form
Solid
- Molecular weight
174.19 Da
- Molecular formula
C7H7FO2S
- PubChem identifier
4784
- Nature
Synthetic
- Solubility
Insoluble in H2O. ≥28.3 mg/mL in EtOH. ≥17.4 mg/mL in DMSO.
- Biochemical name
Phenylmethylsulfonyl fluoride
- Biological description
Non-specific protease inhibitor; binds key active site serine residue in serine proteases, acetylcholinesterases, and thiol proteases. Suitable for use in vitro; in the preparation of cell lysates, and in vivo where it has been shown to block anandamide (ab120429) metabolism. Able to rapidly cross the BBB.
- Canonical SMILES
C1=CC=C(C=C1)CS(=O)(=O)F
- InChI
InChI=1S/C7H7FO2S/c8-11(9,10)6-7-4-2-1-3-5-7/h1-5H,6H2
- InChIKey
YBYRMVIVWMBXKQ-UHFFFAOYSA-N
- IUPAC name
phenylmethanesulfonyl fluoride
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
Notes
Unstable in water
Storage instructions: Hygroscopic, Under inert atmosphere.
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2 product images
Chemical Structure - PMSF, protease inhibitor (ab141032)
2D chemical structure image of ab141032, PMSF, protease inhibitor
Image from Song Y, et al. Plos One, 11(10), e0163165. Fig 5b,; doi: 10.1371/journal.pone.0163165Cellular Activation - PMSF, protease inhibitor (ab141032)
Analysis of protein expression level in the sclera. The scleral tissue was homogenized and solubilized in ice-cold PBS containing PMSF protease inhibitor. Lumican and collagen type I were both overexpressed at the protein level in the transgenic sclera. GAPDH was used as a control. Lane 1 to lane 5, transgenic mice. Lane 6 to lane 9, wild-type mice.
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