MW 211.22 Da, Purity >97%. Negative control for the Src kinase inhibitor PP2 (ab120308). Inhibits EGFR kinase (IC50 = 2.7 μM).
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- Mediators of inflammation 2021:66392522021Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongli Chen,Qiqi Xue,Lijuan Cao,Yanpin Wang,Yuanyuan Chen,Xiaojie Zhang,Fan Xiao,Ying Yang,Melvin R Hayden,Yan Liu,Ke YangPubMed 33927570
- Cell reports 33:1082452020LPS-Induced Acute Kidney Injury Is Mediated by Nox4-SH3YL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Yeon Yoo,Dae Ryong Cha,Borim Kim,Eun Jung An,Sae Rom Lee,Jin Joo Cha,Young Sun Kang,Jung Yeon Ghee,Jee Young Han,Yun Soo BaePubMed 33086058
- The EMBO journal 36:3120-31382017Somatodendritic accumulation of Tau in Alzheimer's disease is promoted by Fyn-mediated local protein translation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanzhou Li et. al.PubMed 28864542
- Nature communications 6:71682015Trans-synaptic zinc mobilization improves social interaction in two mouse models of autism through NMDAR activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEun-Jae Lee et. al.PubMed 25981743
Key facts
- CAS number
- 5334-30-5
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 211.22 Da
- Molecular formula
- C11H9N5
- PubChem identifier
- 4879
- Nature
- Synthetic
Alternative names
2610042N09Rik, 4933409G22Rik, Avian erythroblastic leukemia viral (v erb b) oncogene homolog, Cell growth inhibiting protein 40, Cell proliferation inducing protein 61, DKFZp686O1820, EGFR_HUMAN, ERBB, ERBB1, Epidermal growth factor receptor, Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog), Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian), Errp, FLJ11105, FLJ30129, HER1, NISBD2, NPIK, Oncogen ERBB, P4K2A_HUMAN, P4K2B_HUMAN, PI4 kinase alpha, PI4K 2 beta, PI4K IIb, PI4K alpha, PI4K-beta, PI4K92, PI4KB_HUMAN, PI4KII, PI4KII-BETA, PI4KIIIbeta, PIG61, PIK4, PIK42B, PIK4CA, PIK4CB, Phosphatidylinositol 4 kinase alpha, Phosphatidylinositol 4 kinase catalytic beta, Phosphatidylinositol 4 kinase catalytic beta polypeptide, Phosphatidylinositol 4 kinase wortmannin sensitive, Phosphatidylinositol 4-kinase beta, Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type 2-beta, Phosphatidylinositol 4-kinase type II-alpha, Phosphatidylinositol 4-kinase type II-beta, Proto-oncogene c-ErbB-1, PtdIns 4 kinase, PtdIns 4 kinase alpha, PtdIns 4-kinase beta, Receptor tyrosine-protein kinase ErbB-1, SA7, Species antigen 7, Type II phosphatidylinositol 4 kinase beta isoform, Type III phosphatidylinositol 4 kinase beta, Urogastrone, Wa5, erb-b2 receptor tyrosine kinase 1, mENA, phosphatidylinositol 4 kinase 230, phosphatidylinositol 4 kinase, catalytic, alpha polypeptide, phosphatidylinositol 4 kinase, type III, alpha, pi4K230, v-erb-b Avian erythroblastic leukemia viral oncogen homolog, wa2
Recommended products
MW 211.22 Da, Purity >97%. Negative control for the Src kinase inhibitor PP2 (ab120308). Inhibits EGFR kinase (IC50 = 2.7 μM).
Key facts
- CAS number
- 5334-30-5
- Purity
- > 97%
- Form
- Solid
- Molecular weight
- 211.22 Da
- Molecular formula
- C11H9N5
- PubChem identifier
- 4879
- Nature
- Synthetic
- Solubility
Soluble in ethanol to 25 mM.
Soluble in DMSO to 100 mM.
- Biochemical name
- 1-Phenyl-1h-pyrazolo[3,4-d]pyrimidin-4-amine
- Biological description
Negative control for the Src kinase inhibitor PP2 (ab120308). Inhibits EGFR kinase (IC50 = 2.7 μM).
- Canonical SMILES
- C1=CC=C(C=C1)N2C3=NC=NC(=C3C=N2)N
- InChI
- InChI=1S/C11H9N5/c12-10-9-6-15-16(11(9)14-7-13-10)8-4-2-1-3-5-8/h1-7H,(H2,12,13,14)
- InChIKey
- KKDPIZPUTYIBFX-UHFFFAOYSA-N
- IUPAC name
- 1-phenylpyrazolo[3,4-d]pyrimidin-4-amine
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
EGFR also known as epidermal growth factor receptor is a membrane-bound protein with a mass of approximately 170 kDa. It is expressed in many cell types including epithelial tissues. EGFR has tyrosine kinase activity and plays an important role in the regulation of cell growth and differentiation. PI4K2B PI4 kinase beta also known as PI4KB shares a similar functional landscape. With a mass of about 92 kDa PI4KB localizes mainly in the cytoplasm and trans-Golgi network and participates in phosphatidylinositol (PI) phosphorylation converting PI to phosphatidylinositol 4-phosphate (PI4P). PI4K2A has a function similar to PI4KB in lipid-mediated signaling.
- Biological function summary
EGFR participates in signal transduction cascades that promote cellular proliferation and survival. It often forms homodimers or heterodimers with other receptors in the cell membrane upon ligand binding. PI4K2B acting within lipid microdomains regulates membrane trafficking and assignation of signaling proteins to various cell functions. It does not necessarily work alone and could be a component of larger protein complexes that control phosphorylation dynamics. Both PI4KB and PI4K2A have recognized roles in the maintenance of PI4P levels within cells affecting vesicular trafficking.
- Pathways
EGFR primarily associates with the MAPK and PI3K-Akt signaling pathways influencing cell cycle progression and apoptosis. Key interactions with proteins such as Src enhance downstream signaling and functional outcomes. PI4K2B and PI4KB integrate into pathways that involve phosphatidylinositol metabolism playing roles alongside proteins such as PIP kinase and serving as important nodes in PI3P-related pathways. These connections influence cellular trafficking and organelle function.
- Associated diseases and disorders
EGFR overexpression or mutations relate to cancers notably lung cancer and glioblastoma through altered signaling pathways resulting in unchecked cell division. Its interaction with Src renders it a target for inhibitors like PP2 Src inhibitors in therapeutic investigations. PI4KB dysregulation associates with neurological diseases where disruptions in PI4P balance affect neurodegenerative disease progression. Therefore EGFR and PI4K enzymes are linked to cancer and neurological disorders where their pathways intertwine with disease-specific alterations in protein networks.
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2 product images
Chemical Structure - PP3, Negative control for PP2 (ab120617)
2D chemical structure image of ab120617, PP3, Negative control for PP2
Functional Studies - PP3, Negative control for PP2 (ab120617)
Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker ab11512 staining E cadherin in SW480 cells treated with PP3 (ab120617), by ICC/IF. No change of E cadherin expression with increased concentration of PP3 is observed, as described in literature, since PP3 is the negative control for PP2.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120617 (PP3) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker ab11512 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rat polyclonal antibody (ab98386) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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