PPADS, P2 purinergic receptor antagonist

• FuncS

PubMed

Functional Studies - PPADS, P2 purinergic receptor antagonist (AB120009)

Ca2+ signal from a field A neuron, a field B neuron, and field A astrocytes in response to repetitive episodes of NMDA stimulation (black arrowheads). The NMDA stimulation that evoked an ictal discharge became ineffective after blocking the astrocyte response by bath perfusion with MPEP (ab120008) and PPADS (ab120009). An ictal discharge could be recovered by increasing the number of NMDA puffs (white arrowheads). A double NMDA pulse evoked both astrocyte activation and the ictal discharge after inhibitor washout.

Image from Gómez-Gonzalo M et al., PLoS Biol. 2010;8(4):e1000352. Fig 6(A).; doi: 10.1371/journal.pbio.1000352.

• FuncS

PubMed

Functional Studies - PPADS, P2 purinergic receptor antagonist (AB120009)

Astrocyte Ca2+ signal inhibition does not affect interictal discharges.

(A–D) Mean percentage of astrocytes activated by the ictal discharges (A), mean duration (B) and frequency (C) of the ictal discharge, and mean frequency of interictal discharges (D) under different experimental conditions in EC slice preparations. Controls (n=16), MPEP (ab120008) (n=7), PPADS (ab120009) (n=9), and MPEP+PPADS (n=3). A single asterisk (*) indicates p<0.05; double asterisks (**), p<0.01.

Image from Gómez-Gonzalo M et al., PLoS Biol. 2010;8(4):e1000352. Fig 2.; doi: 10.1371/journal.pbio.1000352. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• Chemical Structure

Lab

Chemical Structure - PPADS, P2 purinergic receptor antagonist (AB120009)

2D chemical structure image of ab120009, PPADS, P2 purinergic receptor antagonist

• CellAct

Unknown

Cellular Activation - PPADS, P2 purinergic receptor antagonist (AB120009)

Inhibition by suramin and PPADS significantly reduces the ATP-induced increase in extracellular H+ flux from isolated Müller cells.
Mean responses to 10 μM ATP with or without suramin and PPADS in the bath; N = 7, error bars represent SEMs.

Image from Tchernookova B, et al. Plos One, 13(2), e0190893. Fig 2c,; doi: 10.1371/journal.pone.0190893