Progesterone, Endogenous progesterone receptor agonist

2 product images

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  Chemical Structure - Progesterone, Endogenous progesterone receptor agonist (ab141252)

  2D chemical structure image of ab141252, Progesterone, Endogenous progesterone receptor agonist

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  Immunocytochemistry/ Immunofluorescence - Progesterone, Endogenous progesterone receptor agonist (ab141252)

  Anti-MDM2 antibody [2A10] ab16895 staining MDM2 in MCF7 cells treated with progesterone (ab141252), by ICC/IF. Decrease in MDM2 expression correlates with increased concentration of progesterone, as described in literature.
  The cells were incubated at 37°C for 24 hour in media containing different concentrations of ab141252 (progesterone) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-MDM2 antibody [2A10] ab16895 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.