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AB141453

Puromycin dihydrochloride, Protein biosynthesis inhibitor

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(8 Publications)

MW 544.4 Da, Purity >98%. Aminonuclease antibiotic agent. Protein biosynthesis inhibitor. Induces cell cycle arrest in G2/M phase and apoptosis. Inhibits transport of proteins into mitochondria. Inhibits translation in vivo.
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Chemical Structure - Puromycin dihydrochloride, Protein biosynthesis inhibitor (AB141453)
  • Chemical Structure

Lab

Chemical Structure - Puromycin dihydrochloride, Protein biosynthesis inhibitor (AB141453)

2D chemical structure image of ab141453, Puromycin dihydrochloride, Protein biosynthesis inhibitor

Key facts

CAS number

58-58-2

Purity

>98%

Form

Solid

form

Molecular weight

544.4 Da

Molecular formula

C<sub>2</sub><sub>2</sub>H<sub>2</sub><sub>9</sub>N<sub>7</sub>O<sub>5</sub>.<sub>2</sub>ClH

PubChem

443311

Nature

Synthetic

Solubility

Soluble in water to 75 mM

Biochemical name

Puromycin dihydrochloride

Biological description

Aminonuclease antibiotic agent. Protein biosynthesis inhibitor. Induces cell cycle arrest in G2/M phase and apoptosis. Inhibits transport of proteins into mitochondria. Inhibits translation in vivo.

Canonical smiles

CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O.Cl.Cl

Isomeric smiles

CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl

InChi

InChI=1S/C22H29N7O5.2ClH/c1-28(2)19-17-20(25-10-24-19)29(11-26-17)22-18(31)16(15(9-30)34-22)27-21(32)14(23)8-12-4-6-13(33-3)7-5-12;;/h4-7,10-11,14-16,18,22,30-31H,8-9,23H2,1-3H3,(H,27,32);2*1H/t14-,15+,16+,18+,22+;;/m0../s1

InChiKey

MKSVFGKWZLUTTO-FZFAUISWSA-N

IUPAC Name

(2S)-2-amino-N-[(2S,3S,4R,5R)-5-[6-(dimethylamino)purin-9-yl]-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl]-3-(4-methoxyphenyl)propanamide;dihydrochloride

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Complementary Products

Biochemicals

AB120081

FCCP, mitochondrial oxidative phosphorylation uncoupler

Chemical Structure - FCCP, mitochondrial oxidative phosphorylation uncoupler (AB120081)

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Biochemicals

AB143145

Rotenone, mitochondrial electron transport chain inhibitor

Chemical Structure - Rotenone, mitochondrial electron transport chain inhibitor (AB143145)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 1860 Puromycin Dihydrochloride. 1860-250 is the same size as the 250 mg size of ab141453.
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Atad5 also known as ELG1 or PCNA unloader is a protein involved in DNA replication and repair. Atad5 has a molecular mass of approximately 215 kDa. You can find Atad5 mainly in the nucleus where DNA replication and repair processes occur. It is expressed in different tissues showing its widespread importance in cellular functions. The protein consists of several domains important for binding other proteins important for its function in maintaining genome stability.
Biological function summary

Atad5 plays an important role in the regulation of replication fork stability and the unloading of PCNA (Proliferating Cell Nuclear Antigen) from DNA. It works as a part of a larger complex involved in DNA replication stress response. Atad5 coordinates protein biosynthesis pathways and ensures the proper completion of the DNA replication process. Oligomyosin domains in Atad5 assist in interacting with other proteins allowing it to perform its functions effectively within the cell's nuclear machinery.

Pathways

Atad5 operates in the DNA damage response and repair processes. It orchestrates the unloading of PCNA from chromatin which is important for DNA synthesis replication fork restart and repair. Atad5 associates with other proteins like RAD17 during these processes ensuring accurate DNA replication and maintaining genomic integrity. Atad5 also affects the ATR-Chk1 signaling pathway reflecting its significant role in cellular stress responses.

Atad5 is linked to chromosomal instability and cancer. Mutations or dysfunctions in Atad5 can lead to uncontrolled or faulty DNA replication contributing to tumorigenesis. Atad5 interacts with proteins such as CHK1 in response to DNA damage and its mutation can disrupt this interaction possibly leading to cancerous growth. Additionally altered Atad5 function is associated with DNA repair disorders leading to heightened sensitivity to DNA-damaging agents and increased risk of disease.
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Product protocols

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Journal of Cancer 15:6434-6451 PubMed39513122

2024

: Expression Patterns, Clinical Correlates and New Target of Papillary Thyroid Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dechao Yin,Kun Wang,Junyu Zhao,Jinming Yao,Xiaofang Han,Bo Yan,Jianjun Dong,Lin Liao

Nature communications 15:7712 PubMed39231947

2024

10-hydroxy-2-decenoic acid prevents osteoarthritis by targeting aspartyl β hydroxylase and inhibiting chondrocyte senescence in male mice preclinically.

Applications

Unspecified application

Species

Unspecified reactive species

Nana Geng,Mengtian Fan,Biao Kuang,Fengmei Zhang,Menglin Xian,Lin Deng,Cheng Chen,Yiming Pan,Jianqiang Chen,Naibo Feng,Li Liang,Yuanlan Ye,Kaiwen Liu,Xiaoli Li,Yu Du,Fengjin Guo

The New phytologist 241:2075-2089 PubMed38095260

2023

Maize DDK1 encoding an Importin-4 β protein is essential for seed development and grain filling by mediating nuclear exporting of eIF1A.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Huang,Yongcai Huang,Li Qin,Qiao Xiao,Qiong Wang,Jiechen Wang,Wenqin Wang,Xiaoduo Lu,Yongrui Wu

Frontiers in cell and developmental biology 10:896014 PubMed35874841

2022

PINK1-Dependent Mitophagy Reduced Endothelial Hyperpermeability and Cell Migration Capacity Under Simulated Microgravity.

Applications

Unspecified application

Species

Unspecified reactive species

Chengfei Li,Yikai Pan,Yingjun Tan,Yongchun Wang,Xiqing Sun

Cellular and molecular life sciences : CMLS 79:280 PubMed35507080

2022

Disruption of ZNF334 promotes triple-negative breast carcinoma malignancy through the SFRP1/ Wnt/β-catenin signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaobo Cheng,Renjie Yu,Li Li,Junhao Mu,Yijia Gong,Fan Wu,Yujia Liu,Xiangyi Zhou,Xiaohua Zeng,Yongzhong Wu,Ran Sun,Tingxiu Xiang

Frontiers in pharmacology 13:890974 PubMed35592418

2022

Autophagy Inhibition Enhances the Anti-Tumor Activity of Methylseleninic Acid in Cisplatin-Resistance Human Lung Adenocarcinoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Xin,Qi Gao,Xindong Xiang,Juanjuan Xu,Yuhan Jiao,Xuan Li,Xianzhen Zhang,Xiuqin Jia

Theranostics 11:805-823 PubMed33391506

2021

Hexosamine biosynthetic pathway promotes the antiviral activity of SAMHD1 by enhancing O-GlcNAc transferase-mediated protein O-GlcNAcylation.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Hu,Qingzhu Gao,Yang Yang,Jie Xia,Wanjun Zhang,Yao Chen,Zhi Zhou,Lei Chang,Yuan Hu,Hui Zhou,Li Liang,Xiaosong Li,Quanxin Long,Kai Wang,Ailong Huang,Ni Tang

Cell death and differentiation 27:1086-1104 PubMed31367013

2019

SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Qingzhu Gao,Guiji Zhang,Yaqiu Zheng,Yi Yang,Chang Chen,Jie Xia,Li Liang,Chong Lei,Yuan Hu,Xuefei Cai,Wenlu Zhang,Hua Tang,Yaxi Chen,Ailong Huang,Kai Wang,Ni Tang
View all publications
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