MW 914.2 Da, Purity >98%. Immunosuppressant and antifungal agent . Complexes with FKBP-12 and inhibits mTOR (mammalian target of rapamycin). In complex with its cellular receptor, the FK506-binding protein (FKBP12), rapamycin binds and inhibits the function of the mammalian target of rapamycin (mTOR).
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- The Journal of pathology 262:441-4532024TRPM2-dependent autophagy inhibition exacerbates oxidative stress-induced CXCL16 secretion by keratinocytes in vitiligo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPan Kang,Yinghan Wang,Jianru Chen,Yuqian Chang,Weigang Zhang,Tingting Cui,Xiuli Yi,Shuli Li,Chunying LiPubMed 38186269
- Lipids in health and disease 22:1562023Sirt6 enhances macrophage lipophagy and improves lipid metabolism disorder by regulating the Wnt1/β-catenin pathway in atherosclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingting Wang,Zheng Cheng,Ran Zhao,Jin Cheng,He Ren,Pengke Zhang,Pengyun Liu,Qimeng Hao,Qian Zhang,Xiaolei Yu,Dongdong Sun,Dongwei ZhangPubMed 37736721
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e230112023Autophagy inhibition mediated by intrauterine miR-1912-3p/CTSD programming participated in the susceptibility to osteoarthritis induced by prenatal dexamethasone exposure in male adult offspring rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuasong Shi,Bin Li,Dingmei Zhang,Hui Han,Hangyuan He,Jiayong Zhu,Hui Wang,Liaobin ChenPubMed 37249374
- The FEBS journal 290:1596-16242022Early loss of endogenous NAD following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnkita Sarkar,Sourav Dutta,Malinki Sur,Semanti Chakraborty,Puja Dey,Piyali MukherjeePubMed 36239430
- Infection and immunity 90:e00016222022Coxiella burnetii Plasmid Effector B Promotes LC3-II Accumulation and Contributes To Bacterial Virulence in a SCID Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengjiao Fu,Jianing Zhang,Mingliang Zhao,Shan Zhang,Lupeng Dai,Xuan Ouyang,Yonghui Yu,Bohai Wen,Dongsheng Zhou,Yansong Sun,Jun Jiao,Xiaolu XiongPubMed 35587202
- Frontiers in immunology 12:7390392022Characterization of Interplay Between Autophagy and Ferroptosis and Their Synergistical Roles on Manipulating Immunological Tumor Microenvironment in Squamous Cell Carcinomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLijie Chen,Xing Niu,Xue Qiao,Sai Liu,Hongmei Ma,Xueqing Shi,Xuemei He,Ming ZhongPubMed 35185859
- Acta biochimica Polonica 69:91-1002022Naringenin and morin reduces insulin resistance and endometrial hyperplasia in the rat model of polycystic ovarian syndrome through enhancement of inflammation and autophagic apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi Yang,Jiuying Liu,Weiwei XuPubMed 35143137
- Journal of neuroinflammation 18:2572021The balance between AIM2-associated inflammation and autophagy: the role of CHMP2A in brain injury after cardiac arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongjiao Shao,Xintao Wang,Tianhua Xu,Yiyang Xia,Derong CuiPubMed 34740380
- Analytical cellular pathology (Amsterdam) 2021:66689472021Activation of MEK1/2/Nrf-2 Signaling Pathway by Epstein-Barr Virus-Latent Membrane Protein 1 Enhances Autophagy and Cisplatin Resistance in T-Cell Lymphoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXintao Jia,Qiuyu He,Mei Zeng,Yuhua Chen,Yan LiuPubMed 34239803
- Autophagy 17:4159-41812021PRDX1 activates autophagy via the PTEN-AKT signaling pathway to protect against cisplatin-induced spiral ganglion neuron damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenwen Liu,Lei Xu,Xue Wang,Daogong Zhang,Gaoying Sun,Man Wang,Mingming Wang,Yuechen Han,Renjie Chai,Haibo WangPubMed 33749526
Key facts
- CAS number
- 53123-88-9
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 914.2 Da
- Molecular formula
- C51H79NO13
- PubChem identifier
- 9854380
- Nature
- Synthetic
Target data
Recommended products
MW 914.2 Da, Purity >98%. Immunosuppressant and antifungal agent . Complexes with FKBP-12 and inhibits mTOR (mammalian target of rapamycin). In complex with its cellular receptor, the FK506-binding protein (FKBP12), rapamycin binds and inhibits the function of the mammalian target of rapamycin (mTOR).
Key facts
- CAS number
- 53123-88-9
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 914.2 Da
- Molecular formula
- C51H79NO13
- PubChem identifier
- 9854380
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 50 mM.
- Biochemical name
- (1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone
- Biological description
Immunosuppressant and antifungal agent . Complexes with FKBP-12 and inhibits mTOR (mammalian target of rapamycin). In complex with its cellular receptor, the FK506-binding protein (FKBP12), rapamycin binds and inhibits the function of the mammalian target of rapamycin (mTOR).
- Canonical SMILES
- CC1CCC2CC(C(=CC=CC=CC(CC(C(=O)C(C(C(=CC(C(=O)CC(OC(=O)C3CCCCN3C(=O)C(=O)C1(O2)O)C(C)CC4CCC(C(C4)OC)O)C)C)O)OC)C)C)C)OC
- Isomeric SMILES
- C[C@@H]1CC[C@H]2C[C@@H](/C(=C/C=C/C=C/[C@H](C[C@H](C(=O)[C@@H]([C@@H](/C(=C/[C@H](C(=O)C[C@H](OC(=O)[C@@H]3CCCCN3C(=O)C(=O)[C@@]1(O2)O)[C@H](C)C[C@H]4CC[C@H]([C@@H](C4)OC)O)C)/C)O)OC)C)C)/C)OC
- InChI
- InChI=1S/C51H79NO13/c1-30-16-12-11-13-17-31(2)42(61-8)28-38-21-19-36(7)51(60,65-38)48(57)49(58)52-23-15-14-18-39(52)50(59)64-43(33(4)26-37-20-22-40(53)44(27-37)62-9)29-41(54)32(3)25-35(6)46(56)47(63-10)45(55)34(5)24-30/h11-13,16-17,25,30,32-34,36-40,42-44,46-47,53,56,60H,14-15,18-24,26-29H2,1-10H3/b13-11+,16-12+,31-17+,35-25+/t30-,32-,33-,34-,36-,37-,38+,39+,40-,42+,43+,44-,46-,47+,51-/m1/s1
- InChIKey
- QFJCIRLUMZQUOT-LBFZIKHJSA-N
- IUPAC name
- (1R,9S,12S,15R,16E,18R,19R,21R,23S,24E,26E,28E,30S,32S,35R)-1,18-dihydroxy-12-[(2R)-1-[(1R,3R,4R)-4-hydroxy-3-methoxycyclohexyl]propan-2-yl]-19,30-dimethoxy-15,17,21,23,29,35-hexamethyl-11,36-dioxa-4-azatricyclo[30.3.1.04,9]hexatriaconta-16,24,26,28-tetraene-2,3,10,14,20-pentone
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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2 product images
Chemical Structure - Rapamycin, mTORC1 complex inhibitor (ab120224)
2D chemical structure image of ab120224, Rapamycin, mTORC1 complex inhibitor
Functional Studies - Rapamycin, mTORC1 complex inhibitor (ab120224)
MCF7 cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of rapamycin (ab120224). Decreased expression of mTOR (phospho S2448) (ab51044) in MCF7 cells correlates with an increase in rapamycin concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab51044 at 1 μg/ml and Anti-beta Actin antibody - Loading Control ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.
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