AB142780

Rhod-2 AM, fluorescent Ca2+ indicator

Name: Rhod-2 AM, fluorescent Ca2+ indicator (CAS 145037-81-6) (ab142780) | Abcam
Brand: Abcam Limited
SKU: AB142780
Price: 230 USD
Availability: InStock
Rating: 5 (3 reviews)

(3 Reviews)

(37 Publications)

Rhod-2 AM (ab142780) is a cell-permeable fluorescent Ca2+ indicator. Binds to Ca2+ (Kd = 570 nM) and fluoresces yellow/orange upon calcium binding. Molecular weight (MW): 1123.9; Purity > 93%

- Suitable to investigate changes in intracellular free Ca2+ concentration
- Excitation/Emission: 552 nm/ 581 nm
- Cited in over 20 publications

1 Images

Chemical Structure - Rhod-2 AM, fluorescent Ca2+ indicator (AB142780)

Chemical Structure

Lab

Chemical Structure - Rhod-2 AM, fluorescent Ca2+ indicator (AB142780)

2D chemical structure image of ab142780, Rhod-2 AM, fluorescent Ca2+ indicator

Key facts

CAS number

145037-81-6

Purity

>93%

Form

Solid

form

Molecular weight

1123.9 Da

Molecular formula

C52H59BrN4O19

PubChem

25229581

Nature

Synthetic

Solubility

Soluble in DMSO to 1 mg/ml

Biochemical name

Rhod-2 AM

Biological description

Cell-permeable fluorescent Ca2+ indicator. Binds to Ca2+ (Kd = 570 nM) and fluoresces yellow/orange upon Ca2+ binding. Cell-impermeable form, Rhod-2 K+ salt (ab142781) also available. Ex550/Em578.

Canonical smiles

CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=CC(=[N+](C)C)C=C4OC5=C3C=CC(=C5)N(C)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-]

InChi

InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52;/h10-17,20-23H,18-19,24-31H2,1-9H3;1H/q+1;/p-1

InChiKey

RWXWZOWDWYQKBK-UHFFFAOYSA-M

IUPAC Name

[9-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium;bromide

Excitation/Emission

Ex: 552nm, Em: 581nm

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Product details

The Ester Rhod-2 once hydrolyzed by cellular endogenous esterases will bind Ca2+ and localizes to mitochondria,

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Properties and storage information

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

It is important to note that this product is reported to be light sensitive|Store in the dark|Store under desiccating conditions

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 58:e14590 PubMed40243820

2025

Oleanolic acid enhanced the anticancer effect of fluorouracil by regulating Ca2+ levels in hepatocellular carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Dong,Yin Gao,Penghui Li,Ping Chen,Yanxin Lv,Yanan Liu,Song Zhang,Minglong Zhang,Yu Wang

Nature cardiovascular research 4:312-329 PubMed39915330

2025

IL-1β enhances susceptibility to atrial fibrillation in mice by acting through resident macrophages and promoting caspase-1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Moreno-Loaiza,Vinicius Cardoso Soares,Manuela de Assumpção Souza,Narendra Vera-Nuñez,Ainhoa Rodriguez de Yurre Guirao,Tatiana Pereira da Silva,Ana Beatriz Pozes,Larissa Perticarrari,Evelin Monteiro,Maria Clara Albino,Sophia Barros Silva,Suelen Silva Gomes Dias,Leonardo Maciel,Humberto Muzi-Filho,Dahienne Ferreira de Oliveira,Bruno Cabral Braga,Luan Pereira Diniz,Mario Costa Cruz,Simone Reis Barbosa,Archimedes Barbosa Castro-Junior,Luciana Conde,Mauro Jorge Cabral-Castro,Olga Ferreira de Souza,Martha Valéria Tavares Pinheiro,Nilson Araújo de Oliveira Junior,Leonardo Rezende de Siqueira,Rodrigo Periquito Cosenza,Claudio Munhoz da Fontoura,Jose Carlos Pizzolante Secco,Juliana da Rocha Ferreira,Andréa Silvestre de Sousa,Denilson Albuquerque,Ronir Raggio Luiz,Pedro Nicolau-Neto,Marco Antonio Pretti,Mariana Boroni,Martin Hernán Bonamino,Tais Hanae Kasai-Brunswick,Debora Bastos Mello,Triciana Gonçalves-Silva,Isalira Peroba Ramos,Fernando A Bozza,João Paulo do Vale Madeiro,Roberto Coury Pedrosa,Marcela Sorelli Carneiro-Ramos,Herculano da Silva Martinho,Patrícia T Bozza,Fernanda Mesquita de Souza,Gabriel Victor Lucena da Silva,Thiago M Cunha,Ilija Uzelac,Flavio Fenton,Renata Moll-Bernardes,Claudia N Paiva,Ariel L Escobar,Emiliano Medei

Journal of molecular and cellular cardiology plus 10:100121 PubMed39697246

2024

Dual calcium-voltage optical mapping of regional voltage and calcium signals in intact murine -R2474S hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Yangpeng Li,Zhu Liu,Christopher O'Shea,Jianhong Li,Xian Luo,Tangting Chen,Xianhong Ou,Weichao Liu,Guoliang Hao,Christopher L-H Huang,Davor Pavlovic,Xiaoqiu Tan,Ming Lei

Theranostics 14:6625-6651 PubMed39479451

2024

Brain-to-heart cholinergic synapse-calcium signaling mediates ischemic stroke-induced atrial fibrillation.

Applications

Unspecified application

Species

Unspecified reactive species

Yingran Liang,Gongxin Wang,Siwen Fan,Junyi Zhang,Shuang He,Guixiang Pan,Guoliang Hao,Yan Zhu

Nature communications 15:7996 PubMed39266508

2024

Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Yang,Lihang Zhu,Shiya Wang,Jixing Gong,Jonathan Nimal Selvaraj,Lincai Ye,Hanxiao Chen,Yaoyao Zhang,Gongxin Wang,Wanjun Song,Zilong Li,Lin Cai,Hao Zhang,Donghui Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2403093 PubMed38896801

2024

CKB Promotes Mitochondrial ATP Production by Suppressing Permeability Transition Pore.

Applications

Unspecified application

Species

Unspecified reactive species

Le He,Jianghua Lin,Shaojuan Lu,Hao Li,Jie Chen,Xinyi Wu,Qixin Yan,Hailiang Liu,Hui Li,Yufeng Shi

Cell death & disease 15:399 PubMed38849335

2024

DDAH-1 maintains endoplasmic reticulum-mitochondria contacts and protects dopaminergic neurons in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yichen Zhao,Weiwei Shen,Minjie Zhang,Min Guo,Yunxiao Dou,Sida Han,Jintai Yu,Mei Cui,Yanxin Zhao

International journal of biological sciences 20:2658-2685 PubMed38725851

2024

Mitochondrial Dysfunction by FADDosome Promotes Gastric Mucosal Injury in Portal Hypertensive Gastropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuelin Xiao,Yiwang Zhang,Kaiduan Xie,Xiaoli Huang,Xianzhi Liu,Jinni Luo,Siwei Tan

Glia 72:1451-1468 PubMed38629411

2024

Astrocytic uptake of posttranslationally modified amyloid-β leads to endolysosomal system disruption and induction of pro-inflammatory signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah Wirth,Annika Schlößer,Antonia Beiersdorfer,Michaela Schweizer,Marcel S Woo,Manuel A Friese,Christian Lohr,Katarzyna M Grochowska

Clinical and translational medicine 14:e1653 PubMed38616702

2024

Mitochondrial dysfunction induced by HIF-1α under hypoxia contributes to the development of gastric mucosal lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Yuelin Xiao,Xianzhi Liu,Kaiduan Xie,Jiajie Luo,Yiwang Zhang,Xiaoli Huang,Jinni Luo,Siwei Tan

View all publications

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Rhod-2 AM, fluorescent Ca2+ indicator

Chemical Structure - Rhod-2 AM, fluorescent Ca2+ indicator (AB142780)

Key facts

CAS number

Purity

Form

Molecular weight

Molecular formula

PubChem

Nature

Solubility

Biochemical name

Biological description

Canonical smiles

InChi

InChiKey

IUPAC Name

Excitation/Emission

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Publications (37)

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Biochemicals

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