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MW 1123.9 Da, Purity >93%. Cell-permeable fluorescent Ca2+ indicator. Binds to Ca2+ (Kd = 570 nM) and fluoresces yellow/orange upon Ca2+ binding. Cell-impermeable form, Rhod-2 K+ salt (ab142781) also available. Ex550/Em578.
- CAS number
145037-81-6
- Purity
> 93%
- Form
Solid
- Molecular weight
1123.9 Da
- Molecular formula
C52H59BrN4O19
- PubChem identifier
25229581
- Nature
Synthetic
- Excitation/Emission
Ex: 552nm, Em: 581nm
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MW 1123.9 Da, Purity >93%. Cell-permeable fluorescent Ca2+ indicator. Binds to Ca2+ (Kd = 570 nM) and fluoresces yellow/orange upon Ca2+ binding. Cell-impermeable form, Rhod-2 K+ salt (ab142781) also available. Ex550/Em578.
Key facts
- CAS number
145037-81-6
- Purity
> 93%
- Form
Solid
- Molecular weight
1123.9 Da
- Molecular formula
C52H59BrN4O19
- PubChem identifier
25229581
- Nature
Synthetic
- Biochemical name
Rhod-2 AM
- Biological description
Cell-permeable fluorescent Ca2+ indicator. Binds to Ca2+ (Kd = 570 nM) and fluoresces yellow/orange upon Ca2+ binding. Cell-impermeable form, Rhod-2 K+ salt (ab142781) also available. Ex550/Em578.
- Canonical SMILES
CC1=CC(=C(C=C1)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C)OCCOC2=C(C=CC(=C2)C3=C4C=CC(=[N+](C)C)C=C4OC5=C3C=CC(=C5)N(C)C)N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C.[Br-]
- InChI
InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52;/h10-17,20-23H,18-19,24-31H2,1-9H3;1H/q+1;/p-1
- InChIKey
RWXWZOWDWYQKBK-UHFFFAOYSA-M
- IUPAC name
[9-[4-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-3-[2-[2-[bis[2-(acetyloxymethoxy)-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-6-(dimethylamino)xanthen-3-ylidene]-dimethylazanium;bromide
- Excitation/Emission
Ex: 552nm, Em: 581nm
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
It is important to note that this product is reported to be light sensitive, Store in the dark, Store under desiccating conditions
Notes
The Ester Rhod-2 once hydrolyzed by cellular endogenous esterases will bind Ca2+ and localizes to mitochondria,
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