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MW 292.4 Da, Purity >98%. p53 activator. Tricyclic thiophene derivative that binds to p53 and induces its accumulation in tumor cells. Prevents p53-HDM2 (MDM2) interaction in vitro and in vivo and affects p53 interaction with several negative regulators. Induces expression of p53 target genes and apoptosis in various tumor cell lines expressing wild-type p53.

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Key facts

CAS number
213261-59-7
Purity
> 98%
Form
Solid
Molecular weight
292.4 Da
Molecular formula
C14H12O3S2
PubChem identifier
374536
Nature
Synthetic

Alternative names

Recommended products

MW 292.4 Da, Purity >98%. p53 activator. Tricyclic thiophene derivative that binds to p53 and induces its accumulation in tumor cells. Prevents p53-HDM2 (MDM2) interaction in vitro and in vivo and affects p53 interaction with several negative regulators. Induces expression of p53 target genes and apoptosis in various tumor cell lines expressing wild-type p53.

Key facts

Purity
> 98%
PubChem identifier
374536
Solubility

Soluble in DMSO to 100 mM.

Soluble in ethanol to 25 mM.

Biochemical name
Rita
Biological description

p53 activator. Tricyclic thiophene derivative that binds to p53 and induces its accumulation in tumor cells. Prevents p53-HDM2 (MDM2) interaction in vitro and in vivo and affects p53 interaction with several negative regulators. Induces expression of p53 target genes and apoptosis in various tumor cell lines expressing wild-type p53.

Canonical SMILES
C1=C(SC(=C1)C2=CC=C(O2)C3=CC=C(S3)CO)CO
InChI
InChI=1S/C14H12O3S2/c15-7-9-1-5-13(18-9)11-3-4-12(17-11)14-6-2-10(8-16)19-14/h1-6,15-16H,7-8H2
InChIKey
KZENBFUSKMWCJF-UHFFFAOYSA-N
IUPAC name
[5-[5-[5-(hydroxymethyl)thiophen-2-yl]furan-2-yl]thiophen-2-yl]methanol

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store in the dark, Store under desiccating conditions

Notes

This product is manufactured by BioVision, an Abcam company and was previously called 2469 RITA. 2469-25 is the same size as the 25 mg size of ab219379.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Interferon beta Vitamin D Receptor (VDR) and ROR gamma are important targets in immune and cellular regulation. Interferon beta with a mass of approximately 20000 Daltons acts as an antiviral cytokine and is produced by fibroblasts and epithelial cells. It is also known as IFN-β and plays a role in controlling viral infections. Vitamin D Receptor is a nuclear hormone receptor expressed in various body tissues including the intestine and bone. VDR's function involves binding to vitamin D which aids in maintaining calcium homeostasis and bone health. ROR gamma also known as RORC (RAR-related orphan receptor C) weighs around 58000 Daltons and is expressed in immune cells particularly affecting the development of Th17 cells.

Biological function summary

Interferon beta modulates the immune response by activating antiviral genes and inhibiting viral replication. It is not part of a complex but acts directly to upregulate MHC class I expression. Vitamin D Receptor plays a role in gene transcription by forming complexes with other transcription factors influencing the expression of genes responsible for calcium absorption. ROR gamma regulates genes involved in metabolic processes and lymphoid tissue development essential in the differentiation of Th17 cells. These Th17 cells are pivotal in inflammatory responses highlighting the ROR gamma's role in immune function.

Pathways

Interferon beta participates in the JAK-STAT signaling pathway activating genes responsible for antiviral defense. It also interacts with proteins such as STAT1 and STAT2 which further amplify the immune response. The Vitamin D Receptor engages significantly in the vitamin D endocrine system which affects numerous signaling pathways including calcium and phosphate metabolism. VDR's interactions with proteins like RXR coactivators enable various gene regulations linked to bone mineralization. ROR gamma is integral to the IL-6 signaling pathway impacting the immune system through interactions with proteins like IL-17 which drive pro-inflammatory responses.

Associated diseases and disorders

Interferon beta plays a therapeutic role in managing multiple sclerosis by downregulating inflammation in the central nervous system. In this context it interacts with proteins such as IL-10 to modulate immune responses. Vitamin D Receptor is linked to bone disorders like osteoporosis where insufficient vitamin D levels can lead to decreased calcium absorption implicating proteins such as osteocalcin. ROR gamma's influence on the differentiation of Th17 cells connects it to autoimmune disorders like rheumatoid arthritis where excessive inflammatory responses occur. Understanding these target-protein relationships contributes to developing targeted therapies for these conditions.

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