(R,S)-3,5-DHPG, group I mGlu receptor agonist

2 product images

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  Chemical Structure - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)

  2D chemical structure image of ab120020, (R,S)-3,5-DHPG, group I mGlu receptor agonist

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  Immunocytochemistry/ Immunofluorescence - (R,S)-3,5-DHPG, group I mGlu receptor agonist (ab120020)

  Anti-FMRP antibody ab17722 staining FMRP in SK-N-SH cells treated with (R,S)-3,5-DHPG (ab120020), by ICC/IF. Increase in FMRP expression correlates with increased concentration of (R,S)-3,5-DHPG, as described in literature.
  The cells were incubated at 37°C for 1h in media containing different concentrations of ab120020 ((R,S)-3,5-DHPG) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-FMRP antibody ab17722 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.