(R,S)-CHPG, mGlu5 agonist

2 product images

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  Chemical Structure - (R,S)-CHPG, mGlu5 agonist (ab120039)

  2D chemical structure image of ab120039, (R,S)-CHPG, mGlu5 agonist

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  Functional Studies - (R,S)-CHPG, mGlu5 agonist (ab120039)

  SK-N-SH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (R,S)-CHPG (ab120039). Increased expression of CREB (phospho S133) in SK-N-SH cells correlates with an increase in (R,S)-CHPG concentration, as described in literature.
  

  Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with Anti-CREB (phospho S133) antibody [E113] ab32096 at 1/500 dilution and Anti-CREB antibody [E306] ab32515 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development solution.