MW 490.1 Da, Purity >98%. Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
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- Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e22026322022Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaishi Yamakawa,Junya Tsuboi,Kousuke Kasahara,Chise Matsuda,Yuhei Nishimura,Tatsuya Kodama,Naoyuki Katayama,Masatoshi Watanabe,Masaki InagakiPubMed 36373718
- Communications biology 4:10662021FBW7 couples structural integrity with functional output of primary cilia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEleni Petsouki,Vasileios Gerakopoulos,Nicholas Szeto,Wenhan Chang,Mary Beth Humphrey,Leonidas TsiokasPubMed 34518642
- The Journal of cell biology 220:2021Time-resolved proteomics profiling of the ciliary Hedgehog response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena A May,Marian Kalocsay,Inès Galtier D'Auriac,Patrick S Schuster,Steven P Gygi,Maxence V Nachury,David U MickPubMed 33856408
- The Journal of biological chemistry 295:14723-147362020Roles of TOG and jelly-roll domains of centrosomal protein CEP104 in its functions in cilium elongation and Hedgehog signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTakashi Yamazoe et. al.PubMed 32820051
- BMB reports 52:619-6242019Deficiency of calpain-6 inhibits primary ciliogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Hye Kim,Do Yeon Kim,Sumin Oh,Je Yeong Ko,Gyuyeong Rah,Kyung Hyun Yoo,Jong Hoon ParkPubMed 31619317
- Developmental biology 455:369-3812019NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSteven Tessier et. al.PubMed 31301300
- Cell reports 24:973-986.e82018The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael E Coulter,Cristina M Dorobantu,Gerrald A Lodewijk,François Delalande,Sarah Cianferani,Vijay S Ganesh,Richard S Smith,Elaine T Lim,C Shan Xu,Song Pang,Eric T Wong,Hart G W Lidov,Monica L Calicchio,Edward Yang,Dilenny M Gonzalez,Thorsten M Schlaeger,Ganeshwaran H Mochida,Harald Hess,Wei-Chung Allen Lee,Maria K Lehtinen,Tomas Kirchhausen,David Haussler,Frank M J Jacobs,Raphael Gaudin,Christopher A WalshPubMed 30044992
- Scientific reports 6:213092016Liver X receptor α is essential for the capillarization of liver sinusoidal endothelial cells in liver injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Xing et. al.PubMed 26887957
- Molecular cancer therapeutics 15:1095-1052016Antagonizing the Hedgehog Pathway with Vismodegib Impairs Malignant Pleural Mesothelioma Growth In Vivo by Affecting Stroma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMayura Meerang et. al.PubMed 26839306
Key facts
- CAS number
- 364590-63-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 490.1 Da
- Molecular formula
- C28H28ClN3OS
- PubChem identifier
- 5284330
- Nature
- Synthetic
Alternative names
FZD11, Frizzled family member 11, GX, Gx protein, Protein Gx, SMOH, SMO_HUMAN, Smoothened frizzled family receptor, Smoothened homolog, Smoothened homolog (Drosophila), Smoothened homolog precursor (SMO) (Gx protein), Smoothened seven transmembrane spanning receptor
Recommended products
MW 490.1 Da, Purity >98%. Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
Key facts
- CAS number
- 364590-63-6
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 490.1 Da
- Molecular formula
- C28H28ClN3OS
- PubChem identifier
- 5284330
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 100 mM.
- Biochemical name
- Smoothened agonist
- Biological description
Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
- Canonical SMILES
- CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl
- InChI
- InChI=1S/C28H28ClN3OS/c1-30-22-9-11-23(12-10-22)32(28(33)27-26(29)24-7-2-3-8-25(24)34-27)18-19-5-4-6-21(17-19)20-13-15-31-16-14-20/h2-8,13-17,22-23,30H,9-12,18H2,1H3
- InChIKey
- VFSUUTYAEQOIMW-UHFFFAOYSA-N
- IUPAC name
- 3-chloro-N-[4-(methylamino)cyclohexyl]-N-[(3-pyridin-4-ylphenyl)methyl]-1-benzothiophene-2-carboxamide
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Smoothened (SMO) is a protein that plays a vital role in the Hedgehog signaling pathway. It is also known by other names such as SMOH and Smoothened homolog. This protein has a molecular mass of approximately 86 kDa. Smoothened is expressed in a variety of tissues including the brain lungs and kidneys. It functions as a transmembrane protein with seven helices which transmits signals across the cell membrane.
- Biological function summary
Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.
- Pathways
Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.
- Associated diseases and disorders
Smoothened has connections to various forms of cancer including basal cell carcinoma and medulloblastoma. Abnormal activation or mutations in Smoothened can lead to unchecked cell proliferation. Smoothened also shows relevance in the development of gliomas. Drugs like 'anti smo' or 'glasdegib' act as Smoothened antagonists aiming to inhibit its function in these diseases by targeting the Hedgehog signaling pathway.
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Chemical Structure - SAG (Smo agonist), Smoothened agonist (ab142160)
2D chemical structure image of ab142160, SAG (Smo agonist), Smoothened agonist
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