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AB142160

SAG (Smo agonist), Smoothened agonist

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(13 Publications)

MW 490.1 Da, Purity >98%. Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.
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Chemical Structure - SAG (Smo agonist), Smoothened agonist (AB142160)
  • Chemical Structure

Lab

Chemical Structure - SAG (Smo agonist), Smoothened agonist (AB142160)

2D chemical structure image of ab142160, SAG (Smo agonist), Smoothened agonist

Key facts

CAS number

364590-63-6

Purity

>98%

Form

Solid

form

Molecular weight

490.1 Da

Molecular formula

C<sub>2</sub><sub>8</sub>H<sub>2</sub><sub>8</sub>ClN<sub>3</sub>OS

PubChem

5284330

Nature

Synthetic

Solubility

Soluble in DMSO to 100 mM

Biochemical name

Smoothened agonist

Biological description

Cell-permeable, selective, potent Smoothened agonist (EC50 = 3 nM). Binds the Smo heptahelical bundle that antagonizes cyclopamine (ab120392) action. Active in vitro and in vivo.

Canonical smiles

CNC1CCC(CC1)N(CC2=CC(=CC=C2)C3=CC=NC=C3)C(=O)C4=C(C5=CC=CC=C5S4)Cl

InChi

InChI=1S/C28H28ClN3OS/c1-30-22-9-11-23(12-10-22)32(28(33)27-26(29)24-7-2-3-8-25(24)34-27)18-19-5-4-6-21(17-19)20-13-15-31-16-14-20/h2-8,13-17,22-23,30H,9-12,18H2,1H3

InChiKey

VFSUUTYAEQOIMW-UHFFFAOYSA-N

IUPAC Name

3-chloro-N-[4-(methylamino)cyclohexyl]-N-[(3-pyridin-4-ylphenyl)methyl]-1-benzothiophene-2-carboxamide

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Complementary Products

Biochemicals

AB120933

Purmorphamine, Smoothened receptor agonist

Chemical Structure - Purmorphamine, Smoothened receptor agonist (AB120933)

  • 0
  • 0
Biochemicals

AB120392

Cyclopamine, Hh signaling inhibitor

Chemical Structure - Cyclopamine, Hh signaling inhibitor (AB120392)

  • 0
  • 0
Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 16
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
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Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smoothened (SMO) is a protein that plays a vital role in the Hedgehog signaling pathway. It is also known by other names such as SMOH and Smoothened homolog. This protein has a molecular mass of approximately 86 kDa. Smoothened is expressed in a variety of tissues including the brain lungs and kidneys. It functions as a transmembrane protein with seven helices which transmits signals across the cell membrane.
Biological function summary

Smoothened influences cellular processes by participating in the Hedgehog signaling pathway. It operates as part of a complex with the Patched (PTCH) receptor. When the Hedgehog ligand binds to PTCH Smoothened becomes activated. This activation leads to changes in gene expression that control cell growth and differentiation. Smoothened is critical in embryonic development tissue regeneration and stem cell maintenance.

Pathways

Smoothened interacts with essential components of the Hedgehog signaling pathway. This pathway includes proteins like PTCH and Gli transcription factors. Smoothened activation triggers the release of these Gli proteins from inhibitory complexes which then migrate to the nucleus to modulate the transcription of target genes. The Hedgehog pathway is important in regulating developmental processes and cellular responses.

Smoothened has connections to various forms of cancer including basal cell carcinoma and medulloblastoma. Abnormal activation or mutations in Smoothened can lead to unchecked cell proliferation. Smoothened also shows relevance in the development of gliomas. Drugs like 'anti smo' or 'glasdegib' act as Smoothened antagonists aiming to inhibit its function in these diseases by targeting the Hedgehog signaling pathway.
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Product protocols

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:2127 PubMed39820844

2025

Loss of Kat2b impairs intraflagellar transport and the Hedgehog signaling pathway in primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Jae Hee Jun,Hwayeon Cha,Je Yeong Ko,Ho-Shik Kim,Kyung Hyun Yoo,Jong Hoon Park

Cellular and molecular life sciences : CMLS 81:75 PubMed38315424

2024

LRP10 and α-synuclein transmission in Lewy body diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Carreras Mascaro,Martyna M Grochowska,Valerie Boumeester,Natasja F J Dits,Ece Naz Bilgiҫ,Guido J Breedveld,Leonie Vergouw,Frank Jan de Jong,Martin E van Royen,Vincenzo Bonifati,Wim Mandemakers

Cellular and molecular life sciences : CMLS 81:70 PubMed38294527

2024

Mirk/Dyrk1B controls ventral spinal cord development via Shh pathway.

Applications

Unspecified application

Species

Unspecified reactive species

N Kokkorakis,K Douka,A Nalmpanti,P K Politis,L Zagoraiou,R Matsas,M Gaitanou

Nature communications 15:365 PubMed38191484

2024

Variants in the WDR44 WD40-repeat domain cause a spectrum of ciliopathy by impairing ciliogenesis initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Accogli,Saurabh Shakya,Taewoo Yang,Christine Insinna,Soo Yeon Kim,David Bell,Kirill R Butov,Mariasavina Severino,Marcello Niceta,Marcello Scala,Hyun Sik Lee,Taekyeong Yoo,Jimmy Stauffer,Huijie Zhao,Chiara Fiorillo,Marina Pedemonte,Maria C Diana,Simona Baldassari,Viktoria Zakharova,Anna Shcherbina,Yulia Rodina,Christina Fagerberg,Laura Sønderberg Roos,Jolanta Wierzba,Artur Dobosz,Amanda Gerard,Lorraine Potocki,Jill A Rosenfeld,Seema R Lalani,Tiana M Scott,Daryl Scott,Mahshid S Azamian,Raymond Louie,Hannah W Moore,Neena L Champaigne,Grace Hollingsworth,Annalaura Torella,Vincenzo Nigro,Rafal Ploski,Vincenzo Salpietro,Federico Zara,Simone Pizzi,Giovanni Chillemi,Marzia Ognibene,Erin Cooney,Jenny Do,Anders Linnemann,Martin J Larsen,Suzanne Specht,Kylie J Walters,Hee-Jung Choi,Murim Choi,Marco Tartaglia,Phillippe Youkharibache,Jong-Hee Chae,Valeria Capra,Sung-Gyoo Park,Christopher J Westlake

Advanced science (Weinheim, Baden-Wurttemberg, Germany) :e2202632 PubMed36373718

2022

Cilia-Mediated Insulin/Akt and ST2/JNK Signaling Pathways Regulate the Recovery of Muscle Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Daishi Yamakawa,Junya Tsuboi,Kousuke Kasahara,Chise Matsuda,Yuhei Nishimura,Tatsuya Kodama,Naoyuki Katayama,Masatoshi Watanabe,Masaki Inagaki

Communications biology 4:1066 PubMed34518642

2021

FBW7 couples structural integrity with functional output of primary cilia.

Applications

Unspecified application

Species

Unspecified reactive species

Eleni Petsouki,Vasileios Gerakopoulos,Nicholas Szeto,Wenhan Chang,Mary Beth Humphrey,Leonidas Tsiokas

The Journal of cell biology 220: PubMed33856408

2021

Time-resolved proteomics profiling of the ciliary Hedgehog response.

Applications

Unspecified application

Species

Unspecified reactive species

Elena A May,Marian Kalocsay,Inès Galtier D'Auriac,Patrick S Schuster,Steven P Gygi,Maxence V Nachury,David U Mick

The Journal of biological chemistry 295:14723-14736 PubMed32820051

2020

Roles of TOG and jelly-roll domains of centrosomal protein CEP104 in its functions in cilium elongation and Hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Yamazoe,Tomoaki Nagai,Shinya Umeda,Yuko Sugaya,Kensaku Mizuno

BMB reports 52:619-624 PubMed31619317

2019

Deficiency of calpain-6 inhibits primary ciliogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hye Kim,Do Yeon Kim,Sumin Oh,Je Yeong Ko,Gyuyeong Rah,Kyung Hyun Yoo,Jong Hoon Park

Developmental biology 455:369-381 PubMed31301300

2019

NFAT5/TonEBP controls early acquisition of notochord phenotypic markers, collagen composition, and sonic hedgehog signaling during mouse intervertebral disc embryogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Steven Tessier,Vedavathi Madhu,Zariel I Johnson,Irving M Shapiro,Makarand V Risbud
View all publications
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