Sodium butyrate, Histone deacetylase inhibitor

• WB

Unknown

Western blot - Sodium butyrate, Histone deacetylase inhibitor (AB120948)

HeLa cells were incubated at 37°C for 6h with vehicle control (0 μM) and different concentrations of sodium butyrate (ab120948). Increased expression of histone H3 (acetyl K27) (ab4729) in HeLa cells correlates with an increase sodium butyrate concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab4729 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

All lanes:

Western blot - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (<a href='/en-us/products/primary-antibodies/histone-h3-acetyl-k27-antibody-chip-grade-ab4729'>ab4729</a>)

Predicted band size: 15 kDa

Observed band size: 17 kDa

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Exposure time: 10s

• Chemical Structure

Lab

Chemical Structure - Sodium butyrate, Histone deacetylase inhibitor (AB120948)

2D chemical structure image of ab120948, Sodium butyrate, Histone deacetylase inhibitor