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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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MW 466.5 Da, Purity >98%. Potent, cell-permeable, broad spectrum inhibitor of protein kinases. Kinases inhibited include: protein kinase C (IC50 = 3 nM), cAMP-dependent protein kinase (IC50 = 8 nM) and p60v-src (IC50 = 6 nM).
Also available as a solid (ab120056).
62996-74-1
> 98%
Solid
466.5 Da
C28H26N4O3
44259
Synthetic
ASV, AW259666, Avian sarcoma virus, C SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, CTCL tumor antigen se20 9, CVID9, D14Ertd420e, EC 2.7.10.2, KPCA_HUMAN, KPCB_HUMAN, KPCD_HUMAN, KPCE_HUMAN, KPCG_HUMAN, KPCT_HUMAN, Kinase PKC delta, LOSK, May-01, MGC125656, MGC125657, MGC126514, MGC141919, MGC49908, MGC57564, Neuronal CSRC tyrosine specific protein kinase, Neuronal SRC, Neuronal proto-oncogene tyrosine-protein kinase Src, OTTHUMP00000043364, OTTHUMP00000043365, OTTHUMP00000067291, OTTHUMP00000174476, OTTHUMP00000174477, Oncogene SRC, PCKd, PKC 0, PKC 2, PKC ZETA, PKC d, PKC delta, PKC epsilon, PKC-A, PKC-B, PKC-alpha, PKC-beta, PKC-gamma, PKCC, PKCE, PKCG, PRKC D, PRKC delta, PRKCA, PRKCB, PRKCB II, PRKCB1, PRKCB2, PRKCE, PRKCG, PRKCT, PRKCZ, Pkcea, Prkcq, Protein Kinase C alpha, Protein Kinase C delta, Protein kinase C, Protein kinase C alpha type, Protein kinase C beta, Protein kinase C beta type, Protein kinase C delta VIII, Protein kinase C delta type, Protein kinase C epsilon, Protein kinase C epsilon type, Protein kinase C gamma, Protein kinase C gamma polypeptide, Protein kinase C gamma type, Protein kinase C theta, Protein kinase C theta type, Protein kinase C zeta, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, Protooncogene SRC Rous sarcoma, SCA 14, SNF1 sucrose nonfermenting like kinase, SRC Oncogene, SRC proto oncogene non receptor tyrosine kinase, SRC_HUMAN, STE20 like serine/threonine-protein kinase, STE20 related kinase, STK-2, Serine/threonine protein kinase 2, Tyrosine kinase pp60c src, Tyrosine protein kinase PRKCD, Tyrosine protein kinase SRC 1, V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian), nPKC-delta, nPKC-epsilon, nPKC-theta, p60-Src, p60c-src, pp60c-src, se20 9, v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog, v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian
MW 466.5 Da, Purity >98%. Potent, cell-permeable, broad spectrum inhibitor of protein kinases. Kinases inhibited include: protein kinase C (IC50 = 3 nM), cAMP-dependent protein kinase (IC50 = 8 nM) and p60v-src (IC50 = 6 nM).
Also available as a solid (ab120056).
ASV, AW259666, Avian sarcoma virus, C SRC, CDNA FLJ14219 fis clone NT2RP3003800 highly similar to Rattus norvegicus tyrosine protein kinase pp60 c src mRNA, CTCL tumor antigen se20 9, CVID9, D14Ertd420e, EC 2.7.10.2, KPCA_HUMAN, KPCB_HUMAN, KPCD_HUMAN, KPCE_HUMAN, KPCG_HUMAN, KPCT_HUMAN, Kinase PKC delta, LOSK, May-01, MGC125656, MGC125657, MGC126514, MGC141919, MGC49908, MGC57564, Neuronal CSRC tyrosine specific protein kinase, Neuronal SRC, Neuronal proto-oncogene tyrosine-protein kinase Src, OTTHUMP00000043364, OTTHUMP00000043365, OTTHUMP00000067291, OTTHUMP00000174476, OTTHUMP00000174477, Oncogene SRC, PCKd, PKC 0, PKC 2, PKC ZETA, PKC d, PKC delta, PKC epsilon, PKC-A, PKC-B, PKC-alpha, PKC-beta, PKC-gamma, PKCC, PKCE, PKCG, PRKC D, PRKC delta, PRKCA, PRKCB, PRKCB II, PRKCB1, PRKCB2, PRKCE, PRKCG, PRKCT, PRKCZ, Pkcea, Prkcq, Protein Kinase C alpha, Protein Kinase C delta, Protein kinase C, Protein kinase C alpha type, Protein kinase C beta, Protein kinase C beta type, Protein kinase C delta VIII, Protein kinase C delta type, Protein kinase C epsilon, Protein kinase C epsilon type, Protein kinase C gamma, Protein kinase C gamma polypeptide, Protein kinase C gamma type, Protein kinase C theta, Protein kinase C theta type, Protein kinase C zeta, Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, Protooncogene SRC, Protooncogene SRC Rous sarcoma, SCA 14, SNF1 sucrose nonfermenting like kinase, SRC Oncogene, SRC proto oncogene non receptor tyrosine kinase, SRC_HUMAN, STE20 like serine/threonine-protein kinase, STE20 related kinase, STK-2, Serine/threonine protein kinase 2, Tyrosine kinase pp60c src, Tyrosine protein kinase PRKCD, Tyrosine protein kinase SRC 1, V src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog (avian), nPKC-delta, nPKC-epsilon, nPKC-theta, p60-Src, p60c-src, pp60c-src, se20 9, v src avian sarcoma (Schmidt Ruppin A2) viral oncogene homolog, v src sarcoma (Schmidt Ruppin A 2) viral oncogene homolog avian
62996-74-1
> 98%
Solid
466.5 Da
C28H26N4O3
44259
Synthetic
Staurosporine
Potent, cell-permeable, broad spectrum inhibitor of protein kinases. Kinases inhibited include: protein kinase C (IC50 = 3 nM), cAMP-dependent protein kinase (IC50 = 8 nM) and p60v-src (IC50 = 6 nM).
Also available as a solid (ab120056).
CC12C(C(CC(O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC
C[C@@]12[C@@H]([C@@H](C[C@@H](O1)N3C4=CC=CC=C4C5=C6C(=C7C8=CC=CC=C8N2C7=C53)CNC6=O)NC)OC
InChI=1S/C28H26N4O3/c1-28-26(34-3)17(29-2)12-20(35-28)31-18-10-6-4-8-14(18)22-23-16(13-30-27(23)33)21-15-9-5-7-11-19(15)32(28)25(21)24(22)31/h4-11,17,20,26,29H,12-13H2,1-3H3,(H,30,33)/t17-,20-,26-,28+/m1/s1
HKSZLNNOFSGOKW-FYTWVXJKSA-N
(2S,3R,4R,6R)-3-methoxy-2-methyl-4-(methylamino)-29-oxa-1,7,17-triazaoctacyclo[12.12.2.12,6.07,28.08,13.015,19.020,27.021,26]nonacosa-8,10,12,14,19,21,23,25,27-nonaen-16-one
Ambient - Can Ship with Ice
+4°C
Store under desiccating conditions, The product can be stored for up to 12 months
PKC isoforms regulate cellular functions like proliferation differentiation and apoptosis. These proteins are part of larger signaling complexes participating in dynamic equilibrium states that determine cellular fate. For example PKC epsilon often interacts within complexes modulating oncogenic pathways while PKC beta 2 plays roles in immune response modulation. The distinct expression patterns and interactions of these isoforms enable them to assume specific biological roles in different tissues.
Protein kinase C (PKC) is a family of serine/threonine kinases essential in cellular signaling. The PKC family includes several isoforms such as PKC delta PKC epsilon PKC beta 2 PKC gamma and PKC theta (also known as PRKCQ). These proteins exhibit diverse molecular masses ranging from about 78 kDa to 82 kDa. PKCs are broadly expressed in various tissues and cells particularly in the brain heart and immune cells. PKC isoforms play significant mechanical roles by phosphorylating various substrate proteins influencing various cellular processes.
Several PKC isoforms engage critical cell signaling pathways such as the MAPK/ERK pathway and the PI3K/AKT pathway. In these pathways PKCs modulate various downstream effectors coordinating signals that influence gene expression and cell survival. PKC delta has significant associations with the MAPK cascade while PKC theta interacts closely with PI3K. These interactions are necessary for linking external signals to intracellular responses thereby acting as converging points for signaling pathways impacting multiple cellular functions.
PKC isoforms associate with cancer and cardiovascular diseases. PKC epsilon often links with cancer progression particularly breast and lung cancers by interacting with oncogenes that promote tumor growth. PKC beta 2 shows connections with metabolic disorders especially in the context of cardiac dysfunction. Disease links may involve other proteins such as Src kinase which interacts with PKC pathways affecting cell motility and survival in cancerous conditions. Understanding these relationships aids in developing therapeutic strategies targeting PKC-related pathways in disease treatment.
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2D chemical structure image of ab120056, Staurosporine, Protein kinase inhibitor
HeLa and Jurkat cells were treated with 1 µM Staurosporine (STS) (ab120056) for 4 hours in complete cell culture media to induce apoptosis and cleaved PARP protein. Untreated and STS treated HeLa and Jurkat lysates were prepared in 1X Cell Extraction Buffer PTR and tested the Cleaved PARP SimpleStepTM ELISA. Raw OD 450 nm values are shown for 500 µg/mL lysate loads.
Lane 1: HeLa, vehicle (DMSO) treated for 4 hours Lane 2: HeLa 1 μM staurpsorine (ab120056), 4 hours Load 20 μg/lane 5% milk/PBST for block and antibody diluent Primary antibodies (2 hours, room temp) All lanes: ab136812 250X Primary Antibodies Cocktail, 1/250 dilution Secondary antibodies (1 hour, room temp) All lanes: ab136812 100X HRP-Conjugated Secondary Antibodies Cocktail, 1/100 dilution
Example of HeLa staurosporine (ab120056) treated cell lysate titration. Background-subtracted data values (mean +/- SD) are graphed.
Example of IC50 determination. HeLa cells were treated with a dose titration of Staurosporine for 4 hours in complete media. Cells were cultured and treated in a 96-well cell culture microtiter plate. Lysates were prepared by direct in-well lysis without media removal: 2X Cell Extraction Buffer PTR was added to an equal volume of media and then resulting lysate was used directly in the Cleaved PARP SimpleStepTM ELISA assay. Raw values for triplicate measurements are plotted. The calculated IC50 is 0.77 μM.
Demonstration of Cleaved PARP capture antibody specificity by western blot assay. 20 μg of HeLa extracts that were untreated or treated for 4 hours with 1 μM Staurosporine were analyzed by western blot. The GAPDH blot is included to show the relative loads of each lysate. In the HeLa cell line, Staurosporine treatment is required to detect cleaved PARP protein, as observed in the SimpleStep ELISA.
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