TMCB, Selective CK2 inhibitor

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - TMCB, Selective CK2 inhibitor (AB120289)

ab23423 staining cIAP2 in HeLa cells treated with TMCB (ab120289), by ICC/IF. Decrease in cIAP2 expression correlates with increased concentration of TMCB, as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120289 (TMCB) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab23423 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

• FuncS

Unknown

Functional Studies - TMCB, Selective CK2 inhibitor (AB120289)

HeLa cells were incubated at 37°C for 24h with vehicle control (0 μM) and different concentrations of TMCB (ab120289). Decreased expression of cIAP2 in HeLa cells correlates with an increase TMCB concentration as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate) 40 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab23423 at 2 μg/ml and ab1801 at 2 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - TMCB, Selective CK2 inhibitor (AB120289)

2D chemical structure image of ab120289, TMCB, Selective CK2 inhibitor