trans-Retinoic acid, Stem cell differentiator
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(6 Publications)
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - trans-Retinoic acid, Stem cell differentiator (AB120728)
ab19857 staining Oct4 in F9 cells treated with trans-retinoic acid (ab120728), by ICC/IF. Decrease of Oct4 expression correlates with increased concentration of trans-retinoic acid, as described in literature.
The cells were incubated at 37°C for 2 days in media containing different concentrations of ab120728 (trans-retinoic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab19857 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- Chemical Structure
Lab
Chemical Structure - trans-Retinoic acid, Stem cell differentiator (AB120728)
2D chemical structure image of ab120728, trans-Retinoic acid, Stem cell differentiator
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Handling instructions
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Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Toxic, refer to SDS for further information.
Publications (6)
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PeerJ 13:e19329 PubMed40292108
2025
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Brain communications 6:fcae244 PubMed39144751
2024
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Nature communications 14:2157 PubMed37061531
2023
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Bioscience reports 39: PubMed30733278
2019
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Acta neuropathologica communications 6:70 PubMed30064522
2018
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Journal of the renin-angiotensin-aldosterone syste 15:243-9 PubMed25031298
2014
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