VU0155041 sodium salt, PAM for mGluR4

• FuncS

Unknown

Functional Studies - VU0155041 sodium salt, PAM for mGluR4 (AB120248)

SKNSH cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of VU0155041 sodium salt (ab120248). Increased expression of Gli1 (ab49314) in SKNSH cells correlates with an decrease in VU0155041 sodium salt concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 30 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab49314 at 2.5 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti- rabbit antibody conjugated to HRP (ab97051) at 1/10000 and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - VU0155041 sodium salt, PAM for mGluR4 (AB120248)

2D chemical structure image of ab120248, VU0155041 sodium salt, PAM for mGluR4