MW 470.6 g/mol, Purity >98%. Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
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- Frontiers in cell and developmental biology 10:7508292022-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomo Shiota,Riko Nagata,Sotaro Kikuchi,Hitoki Nanaura,Masaya Matsubayashi,Mari Nakanishi,Shinko Kobashigawa,Noriyoshi Isozumi,Takao Kiriyama,Kazuaki Nagayama,Kazuma Sugie,Yoshito Yamashiro,Eiichiro MoriPubMed 35399536
- Cell host & microbe 28:853-866.e52020Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMirko Cortese et. al.PubMed 33245857
Key facts
- CAS number
- 5119-48-2
- Purity
- > 98%
- Source
- Withania somnifera
- Molecular weight
- 470.6 g/mol
- Molecular formula
- C28H38O6
- Nature
- Native
Recommended products
MW 470.6 g/mol, Purity >98%. Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
Key facts
- CAS number
- 5119-48-2
- Purity
- > 98%
- Source
- Withania somnifera
- Molecular weight
- 470.6 g/mol
- Molecular formula
- C28H38O6
- Nature
- Native
- Solubility
Soluble in ethanol.
- Biological description
Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
- IUPAC name
- (4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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2 product images
Chemical Structure - Withaferin A from Withania somnifera (ab120644)
2D chemical structure image of ab120644, Withaferin A from Withania somnifera
Functional Studies - Withaferin A from Withania somnifera (ab120644)
ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody.
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