JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB120644

Withaferin A from Withania somnifera

Be the first to review this product! Submit a review

|

(2 Publications)

MW 470.6 g/mol, Purity >98%. Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.
2 Images
Functional Studies - Withaferin A from Withania somnifera (AB120644)
  • FuncS

Unknown

Functional Studies - Withaferin A from Withania somnifera (AB120644)

ab7973 staining Bcl2 in HEK 293 cells treated with withaferin A from Withania somnifera (ab120644), by ICC/IF. Decrease in Bcl2 expression with increased concentration of withaferin A from Withania somnifera, as described in literature.
The cells were incubated at 37°C for 3h in media containing different concentrations of ab120644 (withaferin A from Withania somnifera) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab7973 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

Chemical Structure - Withaferin A from Withania somnifera (AB120644)
  • Chemical Structure

Lab

Chemical Structure - Withaferin A from Withania somnifera (AB120644)

2D chemical structure image of ab120644, Withaferin A from Withania somnifera

Key facts

CAS number

5119-48-2

Purity

>98%

Source

Withania somnifera

Molecular weight

470.6 g/mol

Molecular formula

C<sub>2</sub><sub>8</sub>H<sub>3</sub><sub>8</sub>O<sub>6</sub>

Nature

Native

Solubility

Soluble in ethanol

Biological description

Naturally occurring withanolide/steroidal lactone with anticancer properties. Demonstrates a number of cellular effects including the covalent binding to annexin II, inhibition of chymotrypsin-like activity, PKC and caspase 3. Causes translocation of cyctochrome C to the nucleous and the cleavage of PLC-γ1. Down-regulates Notch 1, 3, cdc25C, phosphorylated Akt and bcl-2 proteins.

IUPAC Name

(4β,5β,6β,22R)-5,6-Epoxy-4,22,27-trihydroxy-1-oxoergosta-2,24-dien-26-oic acid δ-lactone

style
left-column
style
left-column

Properties and storage information

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Store under desiccating conditions|The product can be stored for up to 12 months
style
left-column
style
left-column

Product protocols

style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 10:750829 PubMed35399536

2022

-Derived Proline:Arginine Poly-Dipeptides Modulate Cytoskeleton and Mechanical Stress Response.

Applications

Unspecified application

Species

Unspecified reactive species

Tomo Shiota,Riko Nagata,Sotaro Kikuchi,Hitoki Nanaura,Masaya Matsubayashi,Mari Nakanishi,Shinko Kobashigawa,Noriyoshi Isozumi,Takao Kiriyama,Kazuaki Nagayama,Kazuma Sugie,Yoshito Yamashiro,Eiichiro Mori

Cell host & microbe 28:853-866.e5 PubMed33245857

2020

Integrative Imaging Reveals SARS-CoV-2-Induced Reshaping of Subcellular Morphologies.

Applications

Unspecified application

Species

Unspecified reactive species

Mirko Cortese,Ji-Young Lee,Berati Cerikan,Christopher J Neufeldt,Viola M J Oorschot,Sebastian Köhrer,Julian Hennies,Nicole L Schieber,Paolo Ronchi,Giulia Mizzon,Inés Romero-Brey,Rachel Santarella-Mellwig,Martin Schorb,Mandy Boermel,Karel Mocaer,Marianne S Beckwith,Rachel M Templin,Viktoriia Gross,Constantin Pape,Christian Tischer,Jamie Frankish,Natalie K Horvat,Vibor Laketa,Megan Stanifer,Steeve Boulant,Alessia Ruggieri,Laurent Chatel-Chaix,Yannick Schwab,Ralf Bartenschlager
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB97959

Anti-SOX2 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX2 antibody (AB97959)

  • 4
  • 75
Biochemicals

AB120728

trans-Retinoic acid, Stem cell differentiator

Immunocytochemistry/ Immunofluorescence - trans-Retinoic acid, Stem cell differentiator (AB120728)

  • 0
  • 0
Biochemicals

AB120647

Honokiol, anti-inflammatory and chemotherapeutic agent

Chemical Structure - Honokiol, anti-inflammatory and chemotherapeutic agent (AB120647)

  • 0
  • 0
Primary Antibodies

AB195045

Anti-PAX6 antibody [EPR15858]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX6 antibody [EPR15858] (AB195045)

  • 5
  • 9

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com