MW 284.26 Da, Purity >98%. Cell permeable COX-2 inhibitor (IC50 = 46 μM). Also inhibits CDK7 and CDK9 activity (IC50 values are 12.3 and 0.19 μM for CDK7 and CDK9 respectively). Anti-inflammatory reagent and apoptosis inducer. Active in vivo.
View Alternative Names
Cyclooxygenase, Cyclooxygenase 2b, Cyclooxygenase-2, EC 1.14.99.1, GRIPGHS, Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase, Glucocorticoid-regulated inflammatory cyclooxygenase, Macrophage activation-associated marker protein P71/73, OTTHUMP00000033524, PES-2, PGG/HS, PGH synthase 2, PGH2_HUMAN, PGHS-2, PHS 2, PHS II, PTGS2, Prostaglandin G/H synthase, Prostaglandin G/H synthase 2, Prostaglandin G/H synthase 2 precursor, Prostaglandin G/H synthase and cyclooxygenase, Prostaglandin H2 synthase 2, Prostaglandin-endoperoxide synthase 2, TIS10, TIS10 protein, fj02a10, hCox 2, prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase), ptgs2a, unp1239, wu:fj02a10
- ICC/IF
Unknown
Immunocytochemistry/ Immunofluorescence - Wogonin, COX-2 inhibitor (AB142471)
ab32087 staining MCL1 in HCT116 cells treated with wogonin (ab142471), by ICC/IF. Decrease of MCL1 expression correlates with increased concentration of wogonin, as described in literature.
The cells were incubated at 37°C for 2h in media containing different concentrations of ab142471 (wogonin) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32087 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight® 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
- Chemical Structure
Lab
Chemical Structure - Wogonin, COX-2 inhibitor (AB142471)
2D chemical structure image of ab142471, Wogonin, COX-2 inhibitor
Properties and storage information
Shipped at conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
COX2 plays a significant role in the inflammatory response and is part of the complex process of synthesizing prostaglandins. These compounds mediate inflammation and pain making COX2 an important target for understanding these processes. COX2 is not ubiquitously expressed but rather is induced in activated macrophages and other cells during inflammatory conditions. Its function is also important for normal physiological processes like ovulation and implantation.
Pathways
COX2 is essential in the prostaglandin biosynthesis pathway connecting it to the arachidonic acid metabolism pathway. Cyclooxygenase 2 works with phospholipase A2 which releases arachidonic acid from the phospholipid membrane. COX2 then converts this acid to prostaglandin H2 a precursor for other prostaglandins. COX1 the other isoform of cyclooxygenase is closely related to COX2 and while they have different expression patterns they share some functional similarities in these pathways.
Complementary Products
Primary Antibodies
AB179800
Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012]
KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody [EPR12012] (AB179800)](https://content.abcam.com/products/images/cox2-cyclooxygenase-2-antibody-epr12012-ab179800--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104543.jpg)
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