Rock inhibitor Y-27632 dihydrochloride/Rho kinase inhibitor. A research tool with diverse biological effects, mainly used in stem cell research for maintenance, differentiation, and as a 3D growth matrix or brain organoid differentiation media.
- Cited in over 125 publications
- Purity > 99%
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- Life (Basel, Switzerland) 13:2023Mathematical Modeling of Non-Small-Cell Lung Cancer Biology through the Experimental Data on Cell Composition and Growth of Patient-Derived Organoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRushan Sulimanov,Konstantin Koshelev,Vladimir Makarov,Alexandre Mezentsev,Mikhail Durymanov,Lilian Ismail,Komal Zahid,Yegor Rumyantsev,Ilya LaskovPubMed 38004368
- Nature communications 14:61192023PAX4 loss of function increases diabetes risk by altering human pancreatic endocrine cell development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHwee Hui Lau,Nicole A J Krentz,Fernando Abaitua,Marta Perez-Alcantara,Jun-Wei Chan,Jila Ajeian,Soumita Ghosh,Yunkyeong Lee,Jing Yang,Swaraj Thaman,Benoite Champon,Han Sun,Alokkumar Jha,Shawn Hoon,Nguan Soon Tan,Daphne Su-Lyn Gardner,Shih Ling Kao,E Shyong Tai,Anna L Gloyn,Adrian Kee Keong TeoPubMed 37777536
- Genome biology 24:2072023The relationship between regulatory changes in cis and trans and the evolution of gene expression in humans and chimpanzees.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenneth A Barr,Katherine L Rhodes,Yoav GiladPubMed 37697401
- Frontiers in cell and developmental biology 11:12525212023CAG-expansion mutation results in a dominant negative effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTiago L Laundos,Shu Li,Eric Cheang,Riccardo De Santis,Francesco M Piccolo,Ali H BrivanlouPubMed 37727506
- International journal of medical sciences 20:1326-13352023Electric field promotes dermal fibroblast transdifferentiation through activation of RhoA/ROCK1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenping Wang,Wanqi Huang,Jie Liu,Ze Zhang,Ran Ji,Chao Wu,Jiaping Zhang,Xupin JiangPubMed 37786441
- JACC. Basic to translational science 8:1357-13782023Decreased FAM13B Expression Increases Atrial Fibrillation Susceptibility by Regulating Sodium Current and Calcium Handling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGregory Tchou,Daniela Ponce-Balbuena,Nana Liu,Shamone Gore-Panter,Jeffrey Hsu,Fang Liu,Emmanuel Opoku,Gregory Brubaker,Sarah M Schumacher,Christine S Moravec,John Barnard,David R Van Wagoner,Mina K Chung,Jonathan D SmithPubMed 38094680
- Journal of visualized experiments : JoVE :2023Studying the Epithelial Effects of Intestinal Inflammation In Vitro on Established Murine Colonoids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErin Crawford,Heather L Mentrup,Elizabeth A Novak,Kevin P MollenPubMed 37335108
- JCI insight 8:2023Rescue of GM3 synthase deficiency by spatially controlled, rAAV-mediated ST3GAL5 delivery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuiya Yang,Robert H Brown,Dan Wang,Kevin A Strauss,Guangping GaoPubMed 37014712
- Proceedings of the National Academy of Sciences of the United States of America 120:e22107351202023Rapid cancer cell perineural invasion utilizes amoeboid migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrea R Marcadis,Elizabeth Kao,Qi Wang,Chun-Hao Chen,Laxmi Gusain,Ann Powers,Richard L Bakst,Sylvie Deborde,Richard J WongPubMed 37075074
- Nature aging 3:546-5662023Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHyun Ju Lee,Hafiza Alirzayeva,Seda Koyuncu,Amirabbas Rueber,Alireza Noormohammadi,David VilchezPubMed 37118550
Key facts
- CAS number
- 129830-38-2
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 320.3 Da
- Molecular formula
- C14H23Cl2N3O
- PubChem identifier
- 9901617
- Nature
- Synthetic
Alternative names
CP2D6_HUMAN, CPD6, CYP2D, CYP2D7AP, CYP2D7BP, CYP2D7P2, CYP2D8P2, CYP2DL1, CYPIID6, Cardiolipin activated protein kinase Pak2, Cholesterol 25-hydroxylase, Cytochrome P450 2D6, Cytochrome P450 family 2 subfamily D member 6, Cytochrome P450 family 2 subfamily D polypeptide 6, Cytochrome P450-DB1, Debrisoquine 4-hydroxylase, KIAA0619, KPCE_HUMAN, MGC125656, MGC125657, MGC131603, MGC150606, MGC43611, MGC71074, Microsomal monooxygenase, P450 DB1, P450C2D, PKCE, PKN gamma, PKN2_HUMAN, PRK2, PRKCE, PRKCL2, PRO0435, PRO2042, Pkcea, Protein kinase C epsilon, Protein kinase C epsilon type, Protein kinase C-like 2, Protein kinase N2, Protein-kinase C-related kinase 2, ROCK-I, ROCK1_HUMAN, ROCK2_HUMAN, ROK alpha, Renal carcinoma antigen NY-REN-35, Rho associated coiled coil containing protein kinase 1, Rho associated coiled coil containing protein kinase 2, Rho associated, coiled coil containing protein kinase II, Rho kinase, Rho kinase 2, Rho-alpha kinase, Rho-associated, Rho-associated protein kinase 1, Rho-associated protein kinase 2, Rock II, Rock2m, Rok, Serine/threonine-protein kinase N2, Xenobiotic monooxygenase, coiled-coil-containing protein kinase 1, coiled-coil-containing protein kinase 2, coiled-coil-containing protein kinase I, flavoprotein-linked monooxygenase, nPKC-epsilon, p160 ROCK, p160 ROCK-1, p160 Rhoassociated coiled coil-forming protein kinase, p164 ROCK-2
Recommended products
Rock inhibitor Y-27632 dihydrochloride/Rho kinase inhibitor. A research tool with diverse biological effects, mainly used in stem cell research for maintenance, differentiation, and as a 3D growth matrix or brain organoid differentiation media.
- Cited in over 125 publications
- Purity > 99%
Key facts
- CAS number
- 129830-38-2
- Purity
- > 99%
- Form
- Solid
- Molecular weight
- 320.3 Da
- Molecular formula
- C14H23Cl2N3O
- PubChem identifier
- 9901617
- Nature
- Synthetic
- Biochemical name
- (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridin-4-yl)cyclohexanecarboxamide dihydrochloride
- Biological description
Selective Rho kinase inhibitor.
- Canonical SMILES
- CC(C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl
- Isomeric SMILES
- C[C@H](C1CCC(CC1)C(=O)NC2=CC=NC=C2)N.Cl.Cl
- InChI
- InChI=1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11?,12?;;/m1../s1
- InChIKey
- IDDDVXIUIXWAGJ-DDSAHXNVSA-N
- IUPAC name
- 4-[(1R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide;dihydrochloride
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 2 years
Notes
Y-27632 is a research tool with a wide variety of biological effects, mainly used in the field of stem cell research for maintenance and differentiation. Commonly used as 3D growth matrix component; also a component of brain organoid differentiation media.
How others have used Y-27632 in their research:
Maintenance of tissue culture of iPSCs; 10 μM Y-27632 for 24 hr; Montefiori et al (2018)
Reprogramming of breast cancer cells to induced progenitor-like cells (iPLs) ; 2 μM Y27632 ; Yuan et al (2018)
Differentiation - Y-27632 enables human embryonic stem cells to survive and differentiate into cortical and basal telencephalic progenitors; Watanabe et al (2007)
*Also available in simple stock solutions (Y-27632 dihydrochloride (mM/ml), Rho kinase inhibitor ab144494) - add 1 ml of water to get an exact, ready-to-use concentration.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ROCK1 and ROCK2 also known as Rho-associated coiled-coil containing protein kinases 1 and 2 are serine/threonine kinases with molecular masses of approximately 158 kDa and 160 kDa respectively. These kinases play an essential role in cell structures and functions like contraction motility and adhesion. ROCK1 and ROCK2 express in various tissues including the brain heart and skeletal muscle where they regulate actin cytoskeleton dynamics. ROCK1 and ROCK2 function as effectors for small GTPase RhoA modulating the cytoskeleton through phosphorylation of different substrates.
- Biological function summary
ROCK1 and ROCK2 contribute significantly to the regulation of smooth muscle contraction actin cytoskeleton organization and cellular migration. They exist in their complex forms and primarily act by phosphorylating downstream target proteins which leads to changes in cell shape and movement. Their activity influences various cellular processes such as apoptotic regulation and tissue rigidity which are fundamental in development and homeostasis. Increased levels or activity of ROCKs link to pathological changes including vascular diseases and cancer progression.
- Pathways
ROCK1 and ROCK2 are key components of the Rho/ROCK signaling pathway and the integrin-mediated signaling pathway. These pathways are vital for cellular activities like adhesion migration and proliferation. They often interact with other proteins such as LIM kinase and myosin phosphatase modulating actin-myosin contractility. The Rho/ROCK pathway in particular act as a hub for signal transduction connecting extracellular cues to intracellular responses. This pathway regulates many physiological responses by affecting various cellular substrates and signaling molecules.
- Associated diseases and disorders
ROCK1 and ROCK2 show significant involvement in cardiovascular diseases and cancer. Dysregulation of ROCK activity contributes to hypertension by affecting vascular smooth muscle contraction while aberrant ROCK signaling often results in increased cell migration and invasion in cancerous cells. In hypertension targeting ROCKs can help reduce blood pressure as ROCK inhibitors like dihydrochloride are used therapeutically. Moreover in cancer ROCK1 and ROCK2 interact with proteins such as focal adhesion kinase which lead to enhanced tumor cell migration and metastasis highlighting potential therapeutic targets.
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3 product images
Functional Studies - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)
Anti-VEGFA antibody ab46154 staining VEGF in HeLa cells treated with Y-27632 dihydrochloride (ab120129), by ICC/IF. Decrease in VEGF expression correlates with increased concentration of Y-27632 dihydrochloride, as described in literature.
The cells were incubated at 37°C for 1h in media containing different concentrations of ab120129 (Y-27632 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-VEGFA antibody ab46154 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
Chemical Structure - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)
2D chemical structure image of ab120129, Y-27632 dihydrochloride, Rho kinase inhibitor
Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Y-27632 dihydrochloride, Rho kinase inhibitor (ab120129)
A: Serum-starved human TM cells were incubated on chamber glass slides coated with recombinant human Gal8 in the presence of the ROCK inhibitor ab120129, at different concentrations. After 2 hr, cells were stained with rhodamine-labeled phalloidin, and cells with robust stress fibers were enumerated. Data are expressed as mean±SEM.
B: Cells were treated with an 120129 (Y27632) at 20 μM, stained with rhodamine-labeled phalloidin, and random fields were photographed. Note that cells treated with ab120129 are not spread and exhibit dendrite-like structures.
Credit: Shiri D et al. PLoS One. 2012; 7(7): e41958. doi: 10.1371/journal.pone.0044400
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