Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor

• FuncS

Unknown

Functional Studies - Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (AB120488)

HeLa cells were incubated at 37 °C for 1h with vehicle control (0 μM) and different concentrations of Z-D(OMe)E(OMe)VD(OMe)-FMK (ab120488). After this incubation 10 μM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-D(OMe)E(OMe)VD(OMe)-FMK concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051).

• Chemical Structure

Lab

Chemical Structure - Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor (AB120488)

2D chemical structure image of ab120488, Z-D(OMe)E(OMe)VD(OMe)-FMK, Cell permeable caspase-3 inhibitor