MW 453.5 Da, Purity >94%. Z-VAD(OH)-FMK, Irreversible general caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
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- Molecular and cellular biochemistry 477:2213-22332022Energetic metabolic reprogramming in Jurkat DFF40-deficient cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMerve Kulbay,Bruno Johnson,Guillaume Ricaud,Marie-Noëlle Séguin-Grignon,Jacques BernierPubMed 35460011
- Cell death and differentiation 29:492-5032021Potent pro-apoptotic combination therapy is highly effective in a broad range of cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAntonella Montinaro,Itziar Areso Zubiaur,Julia Saggau,Anna-Laura Kretz,Rute M M Ferreira,Omar Hassan,Ella Kitzig,Ines Müller,Mona A El-Bahrawy,Silvia von Karstedt,Dagmar Kulms,Gianmaria Liccardi,Johannes Lemke,Henning WalczakPubMed 34535764
- Molecular brain 12:782019miR-212-5p attenuates ferroptotic neuronal death after traumatic brain injury by targeting Ptgs2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao Xiao et. al.PubMed 31533781
- Acta pharmacologica Sinica 40:1466-14792019WX20120108, a novel IAP antagonist, induces tumor cell autophagy via activating ROS-FOXO pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Ding et. al.PubMed 31316176
- Nanoscale advances 1:807-8162018Galactose:PEGamine coated gold nanoparticles adhere to filopodia and cause extrinsic apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKonstantina Tzelepi,Cristina Espinosa Garcia,Phil Williams,Jon GoldingPubMed 36132240
- Medical science monitor : international medical jo 24:5247-52572018Receptor-Interacting Protein 3/Caspase-8 May Regulate Inflammatory Response and Promote Tissue Regeneration in the Periodontal Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBingbing Yan et. al.PubMed 30057402
- Cell death and differentiation 25:1457-14722018miR-137 regulates ferroptosis by targeting glutamine transporter SLC1A5 in melanoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeiying Luo et. al.PubMed 29348676
- Journal of virology 92:2018Tumor Necrosis Factor-Mediated Survival of CD169 Cells Promotes Immune Activation during Vesicular Stomatitis Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPrashant V Shinde et. al.PubMed 29142134
- Journal of the American Heart Association 6:2017Rapamycin and CHIR99021 Coordinate Robust Cardiomyocyte Differentiation From Human Pluripotent Stem Cells Via Reducing p53-Dependent Apoptosis.
- PLoS neglected tropical diseases 11:e00059132017Deleterious effect of Usutu virus on human neural cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Salinas et. al.PubMed 28873445
Key facts
- CAS number
- 220644-02-0
- Purity
- > 94%
- Form
- Solid
- Molecular weight
- 453.5 Da
- Molecular formula
- C21H28FN3O7
- PubChem identifier
- 5497170
- Nature
- Synthetic
Recommended products
MW 453.5 Da, Purity >94%. Z-VAD(OH)-FMK, Irreversible general caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
Key facts
- CAS number
- 220644-02-0
- Purity
- > 94%
- Form
- Solid
- Molecular weight
- 453.5 Da
- Molecular formula
- C21H28FN3O7
- PubChem identifier
- 5497170
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 20 mM.
- Biochemical name
- benzyloxycarbonyl-Val-Ala-DL-Asp-fluoromethylketone
- Canonical SMILES
- CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)CF)NC(=O)OCC1=CC=CC=C1
- Isomeric SMILES
- C[C@@H](C(=O)NC(CC(=O)O)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1
- InChI
- InChI=1S/C21H28FN3O7/c1-12(2)18(25-21(31)32-11-14-7-5-4-6-8-14)20(30)23-13(3)19(29)24-15(9-17(27)28)16(26)10-22/h4-8,12-13,15,18H,9-11H2,1-3H3,(H,23,30)(H,24,29)(H,25,31)(H,27,28)/t13-,15?,18-/m0/s1
- InChIKey
- SUUHZYLYARUNIA-LWSHRDBSSA-N
- IUPAC name
- 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoic acid
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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Chemical Structure - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
2D chemical structure image of ab120382, Z-VAD(OH)-FMK, Irreversible general caspase inhibitor
Immunoprecipitation - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
MLKL (phospho S345) was immunoprecipitated from 1mg of L-929 (Mouse connective tissue fibroblast cells) whole cell lysate treated with 20 ng/ml TNF alpha (Recombinant human TNF alpha protein ab9642) + 100 nM Smac mimetic + 20 μM z-VAD compound (ab120382) for 8h using Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 at 1/150 dilution. Western blot was performed from the immunoprecipitate using Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: L-929 whole cell lysate treated with 20 ng/ml TNF alpha (Recombinant human TNF alpha protein ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h;10 μg (Input).
Lane 2: Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 IP in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (Recombinant human TNF alpha protein ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436 in L-929 whole cell lysate treated with 20 ng/ml TNF alpha (Recombinant human TNF alpha protein ab9642) + 100 nM Smac mimetic+ 20 μM z-VAD compound (ab120382) for 8h.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-MLKL (phospho S345) antibody [EPR9515(2)] (Anti-MLKL (phospho S345) antibody [EPR9515(2)] ab196436)
All lanes: Immunoprecipitation - Recombinant human TNF alpha protein (Recombinant human TNF alpha protein ab9642)
Predicted band size: 54 kDa
Observed band size: 54 kDa
Davis J et al. PLoS One. 2013; 8(12): e82053. doi: 10.1371/journal.pone.0082053 Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Functional Studies - Z-VAD(OH)-FMK, Irreversible general caspase inhibitor (ab120382)
ZVAD-fmk treatment does not correct muscular dystrophy in Nol3-/-Sgcd-/- mice.
A: Muscle weights normalized to tibial length of quadriceps of mice treated with or without ZVAD-fmk for 4 weeks
B: Creatine Kinase levels
C: Quantification of the time to exhaustion as assessed by involuntary treadmill running measured from vehicle or ZVAD-fmk treated WT and Nol3-/- Sgcd-/- mice
D: Histologic images taken at 100x of Masson's trichrome stained sections of quadriceps from vehicle or ZVAD-fmk treated WT and Nol3-/-Sgcd-/- mice
Davis J et al. PLoS One. 2013; 8(12): e82053. doi: 10.1371/journal.pone.0082053
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