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MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.

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Images

Chemical Structure - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487), expandable thumbnail
  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487), expandable thumbnail
  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487), expandable thumbnail
  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (AB120487), expandable thumbnail

Publications

Key facts

CAS number

634911-81-2

Purity

> 90%

Form

Solid

Molecular weight

467.5 Da

Molecular formula

C22H30FN3O7

PubChem identifier

5497173

Nature

Synthetic

Recommended products

MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.

Key facts

Purity

> 90%

PubChem identifier

5497173

Biochemical name

methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate

Canonical SMILES

CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

Isomeric SMILES

C[C@@H](C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1

InChI

InChI=1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16?,19-/m0/s1

InChIKey

MIFGOLAMNLSLGH-SXUUOERCSA-N

IUPAC name

methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

-20°C

Storage information

Store under desiccating conditions, The product can be stored for up to 12 months

Product promise

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4 product images

  • Chemical Structure - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487), expandable thumbnail

    Chemical Structure - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)

    2D chemical structure image of ab120487, Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor

  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487), expandable thumbnail

    Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)

    Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).

  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487), expandable thumbnail

    Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)

    HeLa cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-VAD(OMe)-FMK (ab120487). After this incubation 10 μM of camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-VAD(OMe)-FMK concentration, as described in literature.

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 μg /ml and Anti-beta Actin antibody ab8227 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution.

  • Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487), expandable thumbnail

    Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)

    Functional assays: Caspase 3 (active) Red Staining Kit (Cleaved Caspase-3 Staining Kit (Red) ab65617)

    Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 μM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.

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