MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
634911-81-2
> 90%
Solid
467.5 Da
C22H30FN3O7
5497173
Synthetic
MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
634911-81-2
> 90%
Solid
467.5 Da
C22H30FN3O7
5497173
Synthetic
methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
C[C@@H](C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1
InChI=1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16?,19-/m0/s1
MIFGOLAMNLSLGH-SXUUOERCSA-N
methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate
Ambient - Can Ship with Ice
-20°C
Store under desiccating conditions, The product can be stored for up to 12 months
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2D chemical structure image of ab120487, Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor
Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).
HeLa cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-VAD(OMe)-FMK (ab120487). After this incubation 10 μM of camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-VAD(OMe)-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 μg /ml and Anti-beta Actin antibody ab8227 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution.
Functional assays: Caspase 3 (active) Red Staining Kit (Cleaved Caspase-3 Staining Kit (Red) ab65617)
Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 μM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.
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