MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
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- Life science alliance 6:2023FAM111A regulates replication origin activation and cell fitness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana O Rios-Szwed,Vanesa Alvarez,Luis Sanchez-Pulido,Elisa Garcia-Wilson,Hao Jiang,Susanne Bandau,Angus Lamond,Constance AlabertPubMed 37793778
- Cell reports 41:1118262022BCL-xL inhibition potentiates cancer therapies by redirecting the outcome of p53 activation from senescence to apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVijaya Bharti,Reese Watkins,Amrendra Kumar,Rebecca L Shattuck-Brandt,Alexis Mossing,Arjun Mittra,Chengli Shen,Allan Tsung,Alexander E Davies,Walter Hanel,John C Reneau,Catherine Chung,Gina M Sizemore,Ann Richmond,Vivian L Weiss,Anna E VilgelmPubMed 36543138
- The FEBS journal 290:1596-16242022Early loss of endogenous NAD following rotenone treatment leads to mitochondrial dysfunction and Sarm1 induction that is ameliorated by PARP inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnkita Sarkar,Sourav Dutta,Malinki Sur,Semanti Chakraborty,Puja Dey,Piyali MukherjeePubMed 36239430
- Frontiers in pharmacology 13:8848212022Neuronal MD2 induces long-term mental impairments in septic mice by facilitating necroptosis and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongmin Fan,Hongwei Ma,Yi Li,You Wu,Jiajia Wang,Lize Xiong,Zongping Fang,Xijing ZhangPubMed 36016572
- Cell death & disease 13:6622022Hypoxia aggravates ferroptosis in RPE cells by promoting the Fenton reaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoshiyuki Henning,Ursula Sarah Blind,Safa Larafa,Johann Matschke,Joachim FandreyPubMed 35906211
- EMBO reports 21:e506622020FAM111 protease activity undermines cellular fitness and is amplified by gain-of-function mutations in human disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaskia Hoffmann et. al.PubMed 32776417
- Molecular & cellular proteomics : MCP 19:1236-12472020Immunopeptidomic Analysis Reveals That Deamidated HLA-bound Peptides Arise Predominantly from Deglycosylated Precursors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShutao Mei et. al.PubMed 32357974
- PLoS pathogens 16:e10081052020Proteasomal inhibition triggers viral oncoprotein degradation via autophagy-lysosomal pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChandrima Gain et. al.PubMed 32092124
- Reproductive biomedicine online 40:261-2692020Testicular tissue cryopreservation is the preferred method to preserve spermatogonial stem cells prior to transplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJaime Onofre,Prashant Kadam,Yoni Baert,Ellen GoossensPubMed 32001160
- Cancers 11:2019Butoxy Mansonone G Inhibits STAT3 and Akt Signaling Pathways in Non-Small Cell Lung Cancers: Combined Experimental and Theoretical Investigations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPanupong Mahalapbutr et. al.PubMed 30925736
Key facts
- CAS number
634911-81-2
- Purity
> 90%
- Form
Solid
- Molecular weight
467.5 Da
- Molecular formula
C22H30FN3O7
- PubChem identifier
5497173
- Nature
Synthetic
Recommended products
MW 467.5 Da, Purity >90%. Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
Key facts
- CAS number
634911-81-2
- Purity
> 90%
- Form
Solid
- Molecular weight
467.5 Da
- Molecular formula
C22H30FN3O7
- PubChem identifier
5497173
- Nature
Synthetic
- Biochemical name
methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate
- Canonical SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1
- Isomeric SMILES
C[C@@H](C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)OCC1=CC=CC=C1
- InChI
InChI=1S/C22H30FN3O7/c1-13(2)19(26-22(31)33-12-15-8-6-5-7-9-15)21(30)24-14(3)20(29)25-16(17(27)11-23)10-18(28)32-4/h5-9,13-14,16,19H,10-12H2,1-4H3,(H,24,30)(H,25,29)(H,26,31)/t14-,16?,19-/m0/s1
- InChIKey
MIFGOLAMNLSLGH-SXUUOERCSA-N
- IUPAC name
methyl 5-fluoro-3-[[(2S)-2-[[(2S)-3-methyl-2-(phenylmethoxycarbonylamino)butanoyl]amino]propanoyl]amino]-4-oxopentanoate
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Store under desiccating conditions, The product can be stored for up to 12 months
Product promise
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4 product images
Chemical Structure - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
2D chemical structure image of ab120487, Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor
Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
Titration of the Caspase inhibitor Z-VAD(OMe)-FMK (ab120487) (duplicates; +/- SD).
Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
HeLa cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-VAD(OMe)-FMK (ab120487). After this incubation 10 μM of camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic HeLA cells correlates with an increase in Z-VAD(OMe)-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab37722 at 1 μg /ml and Anti-beta Actin antibody ab8227 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution.
Functional Studies - Z-VAD(OMe)-FMK, Cell permeable, irreversible pan-caspase inhibitor (ab120487)
Functional assays: Caspase 3 (active) Red Staining Kit (Cleaved Caspase-3 Staining Kit (Red) ab65617)
Caspase 3 activity in Jurkat cells (3 x10e5 cells) following 24 hour exposure to 2 μM Camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) with or without 50 μM caspase inhibitor Z-VAD(OMe)-FMK (ab120487). Background signal subtracted, duplicates; +/- SD.
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