Z-VDVAD-FMK, caspase-2 inhibitor

2 product images

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  Chemical Structure - Z-VDVAD-FMK, caspase-2 inhibitor (ab142035)

  2D chemical structure image of ab142035, Z-VDVAD-FMK, caspase-2 inhibitor

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  Functional Studies - Z-VDVAD-FMK, caspase-2 inhibitor (ab142035)

  Anti-Caspase-3 antibody ab13847 staining active caspase 3 in SKNSH cells treated with Z-VDVAD-FMK (ab142035), by ICC/IF. Decrease in active caspase 3 expression correlates with increased concentration of Z-VDVAD-FMK, as described in literature.
  The cells were incubated at 37°C for 1 hour in media containing different concentrations of ab142035 (Z-VDVAD-FMK) in DMSO. After this incupation 10 µM of camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) was added to all samples and the cells were incubated for further 24 hours. The samples were then fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with Anti-Caspase-3 antibody ab13847 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with