MW 630.7 Da, Purity >98%. Z-YVAD-FMK, Irreversible caspase-1 inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
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- Journal of virology 96:e02127212022PRRSV Infection Induces Gasdermin D-Driven Pyroptosis of Porcine Alveolar Macrophages through NLRP3 Inflammasome Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng He,Lu Li,Haifan Chen,Xiaoli Hu,Wendi Wang,Hui Zhang,Ruiping Wei,Xiaoxiao Zhang,Yaosheng Chen,Xiaohong LiuPubMed 35758658
- Molecular neurodegeneration 15:262020NLRP12 collaborates with NLRP3 and NLRC4 to promote pyroptosis inducing ganglion cell death of acute glaucoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Chen et. al.PubMed 32295623
- The Journal of allergy and clinical immunology 145:1690-1693.e52020CRISPR gene-engineered CYBB THP-1 cell lines highlight the crucial role of NADPH-induced reactive oxygen species for regulating inflammasome activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAissa Benyoucef et. al.PubMed 31954113
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:1819-18322020Cigarette smoke inhibits the NLRP3 inflammasome and leads to caspase-1 activation via the TLR4-TRIF-caspase-8 axis in human macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Buscetta,Serena Di Vincenzo,Monica Miele,Ester Badami,Elisabetta Pace,Chiara CipollinaPubMed 31914643
- RNA biology 17:335-3492019NLRP3 inflammasome activation by Foot-and-mouth disease virus infection mainly induced by viral RNA and non-structural protein 2B.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoying Zhi,Yun Zhang,Shiqi Sun,Zhihui Zhang,Hu Dong,Xin Luo,Yanquan Wei,Zengjun Lu,Yongxi Dou,Run Wu,Zhengfan Jiang,Changjiang Weng,Ho Seong Seo,Huichen GuoPubMed 31840571
- EBioMedicine 47:578-5892019Benzene metabolites trigger pyroptosis and contribute to haematotoxicity via TET2 directly regulating the Aim2/Casp1 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Guo et. al.PubMed 31474553
- Frontiers in immunology 9:26332018Cell Death Is Counteracted by Mitophagy in HIV-Productively Infected Astrocytes but Is Promoted by Inflammasome Activation Among Non-productively Infected Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiego S Ojeda et. al.PubMed 30515154
- Archives of oral biology 93:115-1252018Differences in interleukin-1β release-inducing activity of Candida albicans toward dendritic cells and macrophages.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Hasebe et. al.PubMed 29894908
- Experimental cell research 366:1-152018ATP/P2X7-NLRP3 axis of dendritic cells participates in the regulation of airway inflammation and hyper-responsiveness in asthma by mediating HMGB1 expression and secretion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuiting Li et. al.PubMed 29545090
- The Journal of allergy and clinical immunology 139:1641-1649.e62016Mammalian target of rapamycin inhibition counterbalances the inflammatory status of immune cells in patients with chronic granulomatous disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAurélie Gabrion,Isabelle Hmitou,Despina Moshous,Bénédicte Neven,Alain Lefèvre-Utile,Jean-Sébastien Diana,Félipe Suarez,Capucine Picard,Stéphane Blanche,Alain Fischer,Marina Cavazzana,Fabien TouzotPubMed 27702670
Key facts
- Purity
> 98%
- Form
Lyophilized
- Molecular weight
630.7 Da
- Molecular formula
C31H39FN4O9
- PubChem identifier
16760349
- Nature
Synthetic
Recommended products
MW 630.7 Da, Purity >98%. Z-YVAD-FMK, Irreversible caspase-1 inhibitor. Achieve your results faster with highly validated, pure and trusted compounds.
Key facts
- Purity
> 98%
- Form
Lyophilized
- Molecular weight
630.7 Da
- Molecular formula
C31H39FN4O9
- PubChem identifier
16760349
- Nature
Synthetic
- Solubility
Soluble in DMSO to 20 mM.
- Biochemical name
Caspase-1 Inhibitor VI
- Canonical SMILES
CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)OCC2=CC=CC=C2
- Isomeric SMILES
C[C@@H](C(=O)N[C@@H](CC(=O)OC)C(=O)CF)NC(=O)[C@H](C(C)C)NC(=O)C(CC1=CC=C(C=C1)O)NC(=O)OCC2=CC=CC=C2
- InChI
InChI=1S/C31H39FN4O9/c1-18(2)27(30(42)33-19(3)28(40)34-23(25(38)16-32)15-26(39)44-4)36-29(41)24(14-20-10-12-22(37)13-11-20)35-31(43)45-17-21-8-6-5-7-9-21/h5-13,18-19,23-24,27,37H,14-17H2,1-4H3,(H,33,42)(H,34,40)(H,35,43)(H,36,41)/t19-,23-,24?,27-/m0/s1
- InChIKey
MVPQJUFFTWWKBT-UYDLRUHXSA-N
- IUPAC name
methyl (3S)-5-fluoro-3-[[(2S)-2-[[(2S)-2-[[3-(4-hydroxyphenyl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-3-methylbutanoyl]amino]propanoyl]amino]-4-oxopentanoate
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate long-term storage conditions
-20°C
- Storage information
Store under desiccating conditions, The product can be stored for up to 12 months
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 1141 YVAD-FMK, Z-,. 1141-5 is the same size as the 5 mg size of ab141388.
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3 product images
Chemical Structure - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
2D chemical structure image of ab141388, Z-YVAD-FMK, Irreversible caspase-1 inhibitor
Image from Migita K, et al. Plos One, 9(5), e96703. Fig 3a,; doi: 10.1371/journal.pone.0096703Cellular Activation - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
Neutrophils were stimulated with SAA (5 µg/ml) in the presence or absence of Z-YVAD-FMK for 8 h. After stimulation, supernatants were analyzed for IL-1 Β production using ELISA. Values represent the mean ± SD of two independent experiments. *p<0.001 compared to SAA-stimulated neutrophils.
Functional Studies - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (ab141388)
SHSY5Y cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-YVAD-FMK (ab141388). After this incubation 10 μM of camptothecin (Camptothecin, DNA topoisomerase inhibitor ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-YVAD-FMK concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and Anti-beta Actin antibody ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution and visualised using ECL development so
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