Z-YVAD-FMK, Irreversible caspase-1 inhibitor

• FuncS

Unknown

Functional Studies - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (AB141388)

SHSY5Y cells were incubated at 37°C for 1h with vehicle control (0 μM) and different concentrations of Z-YVAD-FMK (ab141388). After this incubation 10 μM of camptothecin (ab120115) was added to all samples and the cells were incubated for further 24h. Increased expression of full length PARP (ab37722) in camptothecin induced apoptotic SHSY5Y cells correlates with an increase in Z-YVAD-FMK concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab37722 at 1 μg/ml and ab8227 at 1 μg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development so

• CellAct

Unknown

Cellular Activation - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (AB141388)

Neutrophils were stimulated with SAA (5 μg/ml) in the presence or absence of Z-YVAD-FMK for 8 h. After stimulation, supernatants were analyzed for IL-1 β production using ELISA. Values represent the mean ± SD of two independent experiments. *p<0.001 compared to SAA-stimulated neutrophils.

Image from Migita K, et al. Plos One, 9(5), e96703. Fig 3a,; doi: 10.1371/journal.pone.0096703

• Chemical Structure

Lab

Chemical Structure - Z-YVAD-FMK, Irreversible caspase-1 inhibitor (AB141388)

2D chemical structure image of ab141388, Z-YVAD-FMK, Irreversible caspase-1 inhibitor