MW 271.28 g/mol, Purity >98%. Specific cGMP phosphodiesterase inhibitor, selective for PDE6, 5, 11 and 9 (IC50 values are 0.15 (PDE6), 0.76 (PDE5), 12 (PDE11) and 29 μM (PDE9)). Reputed GPR35 agonist (EC50 values are 16 and 840 nM at rat and human GPR35 receptors, respectively). Important mediator for a variety of cellular processes including vasodilation.
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Key facts
- CAS number
- 37762-06-4
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 271.28 g/mol
- Molecular formula
- C13H13N5O2
- Nature
- Synthetic
Recommended products
MW 271.28 g/mol, Purity >98%. Specific cGMP phosphodiesterase inhibitor, selective for PDE6, 5, 11 and 9 (IC50 values are 0.15 (PDE6), 0.76 (PDE5), 12 (PDE11) and 29 μM (PDE9)). Reputed GPR35 agonist (EC50 values are 16 and 840 nM at rat and human GPR35 receptors, respectively). Important mediator for a variety of cellular processes including vasodilation.
Key facts
- CAS number
- 37762-06-4
- Purity
- > 98%
- Form
- Solid
- Molecular weight
- 271.28 g/mol
- Molecular formula
- C13H13N5O2
- Nature
- Synthetic
- Solubility
Soluble in DMSO to 25 mM.
- Biological description
Specific cGMP phosphodiesterase inhibitor, selective for PDE6, 5, 11 and 9 (IC50 values are 0.15 (PDE6), 0.76 (PDE5), 12 (PDE11) and 29 μM (PDE9)). Reputed GPR35 agonist (EC50 values are 16 and 840 nM at rat and human GPR35 receptors, respectively). Important mediator for a variety of cellular processes including vasodilation.
- Canonical SMILES
- CCCOC1CCCCC1C1[NH]C(=O)C2NN[NH]C2N1
- IUPAC name
- 3,6-Dihydro-5-(2-propoxyphenyl)-7H-1,2,3-triazolo[4,5-d]pyrimidin-7-one
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- Store under desiccating conditions, The product can be stored for up to 12 months
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2 product images
Chemical Structure - Zaprinast, cGMP phosphodiesterase inhibitor (ab120940)
2D chemical structure image of ab120940, Zaprinast, cGMP phosphodiesterase inhibitor
Functional Studies - Zaprinast, cGMP phosphodiesterase inhibitor (ab120940)
ab76165 staining ERK1 + ERK2 (phospho T202 + Y204) in U87MG cells treated with zaprinast (ab120940), by ICC/IF. Increase of ERK1 + ERK2 (phospho T202 + Y204) expression correlates with increased concentration of zaprinast, as described in literature.
The cells were incubated at 37°C for 24 hours in media containing different concentrations of ab120940 (zaprinast) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab76165 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
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