ZK 756326, non-peptide CCR8 chemokine receptor agonist

• FuncS

Unknown

Functional Studies - ZK 756326, non-peptide CCR8 chemokine receptor agonist (AB120814)

THP1 cells were incubated at 37°C for 3 minutes with vehicle control (0 μM) and different concentrations of ZK 756326 (ab120814). Increased expression of ERK1 + ERK2 (phospho T202 + Y204 + T185 + Y187) (ab32538) in THP1 cells correlates with an increase in ZK 756326 concentration, as described in literature.

Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab32538 at 1/500 dilution and ab17942 at 1 μg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.

• Chemical Structure

Lab

Chemical Structure - ZK 756326, non-peptide CCR8 chemokine receptor agonist (AB120814)

2D chemical structure image of ab120814, ZK 756326, non-peptide CCR8 chemokine receptor agonist