Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
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Publications
- Nature communications 15:6062024DNMT3A clonal hematopoiesis-driver mutations induce cardiac fibrosis by paracrine activation of fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMariana Shumliakivska,Guillermo Luxán,Inga Hemmerling,Marina Scheller,Xue Li,Carsten Müller-Tidow,Bianca Schuhmacher,Zhengwu Sun,Andreas Dendorfer,Alisa Debes,Simone-Franziska Glaser,Marion Muhly-Reinholz,Klara Kirschbaum,Jedrzej Hoffmann,Eike Nagel,Valentina O Puntmann,Sebastian Cremer,Florian Leuschner,Wesley Tyler Abplanalp,David John,Andreas M Zeiher,Stefanie DimmelerPubMed 38242884
- Molecular neurobiology 61:2672-26852023Sigma-1 Receptor Inhibition Reduces Mechanical Allodynia and Modulate Neuroinflammation in Chronic Neuropathic Pain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Denaro,Lorella Pasquinucci,Rita Turnaturi,Cristiana Alberghina,Lucia Longhitano,Sebastiano Giallongo,Giuliana Costanzo,Salvatore Spoto,Margherita Grasso,Agata Zappalà,Giovanni Li Volti,Daniele Tibullo,Nunzio Vicario,Rosalba Parenti,Carmela ParentiPubMed 37922065
Key facts
- Applications
ICC/IF, IHC (PFA fixed), BL, IHC-Fr, IHC-P
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes Use at 10% dilution. |
Application IHC (PFA fixed) | Reactivity Reacts | Dilution info - | Notes - |
Application BL | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
Key facts
- Applications
ICC/IF, IHC (PFA fixed), BL, IHC-Fr, IHC-P
- Form
Liquid
- Storage buffer
Constituents: Whole serum
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Normal donkey serum ab7475 is used extensively for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies.
The donkey serum blocks the binding of Fc receptors in the sample to the primary and secondary antibodies used in the experiment, and also blocks non-specific binding of the antibodies to the sample.
Typically the serum used for blocking is from a different species than the species in which the primary antibody was raised. Often the blocking serum is from the species in which the secondary antibody was raised.
Serum can be used directly for blocking, or as a constituent of a blocking buffer.
Strain: Mixed breed and sex.
Raised in: Donkey
Purity: Whole antiserum
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2 product images
Paul, Rajib et al., PloS one vol. 12,2 e0171285., Fig 5, doi:10.1371/journal.pone.0171285Functional Studies - Normal Donkey Serum (ab7475)
The sections were rinsed three times with 0.1 M Tris-buffered saline (TBS, 0.1 M; pH 7.4), incubated in 3% H2O2 in TBS, permeabilized with 0.3% Triton X-100, and blocked with 10% donkey serum containing 0.3% Triton X-100. The sections were incubated overnight with primary antibody for TH (1:700) in TBS, containing 2% donkey serum (ab7475) at 4°C and then incubated with HRP-conjugated secondary antibody (1:1000) in TBS for 1 h at room temperature.
Krzy?anowska, Weronika et al., PloS one?vol. 12,10 e0186243., Fig 6, doi:10.1371/journal.pone.0186243Functional Studies - Normal Donkey Serum (ab7475)
Anti-xCT rabbit (Anti-xCT antibody ab37185 Abcam) dilution: 1:200 Donkey anti-rabbit TR (Donkey Anti-Rabbit IgG H&L (Texas Red ®) ab6800 Abcam) dilution: 1:1000 Donkey normal serum (ab7475 Abcam) dilution: 10%
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