100x Citrate Buffer pH 6.0

2 product images

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  Jujo T et al. PLoS One (2014) Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 100x Citrate Buffer pH 6.0 (ab64236)

  Immunohistochemical analysis of mouse lung. Samples were fixed in 10% buffered formalin, paraffinized and sliced at 1.5 μm thick. Antigen retrieval was performed using 10x Citrate Buffer pH 6.0 ab64214 for the deparaffinized slices. Sections were blocked with 2% normal goat serum, PBS(-) and 0.1% Tween20. They were then incubated with the primary antibodies for 1 hour at 4°C and with secondary antibodies for 30 minutes at room temperature. The avidin-biotin-peroxidase complex method with peroxidase streptavidin and the DAB substrate kit DAB Substrate Kit ab64238 was performed.
  

  (A) A resected lung from a mouse sacrificed 28 days after SCL injection.

  (B) Haemotoxylin and Eosin staining showing the tumor composed of a central area with necrosis and a peripheral zone filled with SCLs.

  (C) Immunohistochemical staining for MMP-14 showing positive expression of MMP-14 in the peripheral zone of the tumor and negative in central zone.

  (D) Immunohistochemical staining weak staining of MMP-2 in the peripheral zone.

  This image was produced using 10x Citrate Buffer pH 6.0 ab64214 (a lower concentration of ab64236 ). Therefore, we expect ab64236 to work as well when diluted to the required concentration.

• 

  Jujo T et al. PLoS One (2014)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 100x Citrate Buffer pH 6.0 (ab64236)

  Immunohistochemical analysis of liver tissue section labeling PCNA with Anti-PCNA antibody ab18197 at 1/250. Antigen retrieval was performed with antigen retrieval buffer, ab64236. A and B, PCNA staining in right and left grafts 1 day after reperfusion. E and F, PCNA staining in right and left grafts 5 day after reperfusion.