10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding.
- Cited in over 30 publications
- Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB)
- 100% casein blocking as preservative
Images
Publications
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- Frontiers in physiology 14:13018042023Characterization of wooden breast myopathy: a focus on syndecans and ECM remodeling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLucie Pejšková,Sissel Beate Rønning,Matthew Peter Kent,Nina Therese Solberg,Vibeke Høst,To Thu-Hien,Jens Petter Wold,Marianne Lunde,Ellen Mosleth,Addolorata Pisconti,Svein Olav Kolset,Cathrine Rein Carlson,Mona Elisabeth PedersenPubMed 38130476
- Biosensors 13:2023Gradient Guided-Mode Resonance Biosensor with Smartphone Readout.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing-Zhou Lin,Cheng-Hao Chen,Yuan-Pei Lei,Cheng-Sheng HuangPubMed 38131766
- Current issues in molecular biology 45:6916-69262023Hexavalent Chromium Inhibited Zebrafish Embryo Development by Altering Apoptosis- and Antioxidant-Related Genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhoa Dang Dang,Chi Nguyen Quynh Ho,Huy Duc Van,Son Thanh Dinh,Quynh Thi Truc Nguyen,Tram Thi Thuy Nguyen,Xuyen Thi Ngoc Kien,Tuyet Van Dao,Hung Viet Nong,Minh Thai Nguyen,Chung Chinh Doan,Son Nghia Hoang,Thao Thi Phuong Nguyen,Long Thanh LePubMed 37623255
- Journal of ginseng research 47:440-4472023Ginsenoside Re prevents 3-methyladenine-induced catagen phase acceleration by regulating Wnt/β-catenin signaling in human dermal papilla cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGyusang Jeong,Seung Hyun Shin,Su Na Kim,Yongjoo Na,Byung Cheol Park,Jeong Hun Cho,Won-Seok Park,Hyoung-June KimPubMed 37252273
- Journal of experimental zoology. Part A, Ecological and integrative physiology 339:451-4632023Salinity-dependent changes in branchial morphometry and Na , K -ATPase responses of euryhaline Asian sea bass, Lates calcarifer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Chen Ding,Sih-Shien Lee,Sheng-Kai Peng,Wen-Kai Yang,Tsung-Han LeePubMed 36878859
- Open medicine (Warsaw, Poland) 18:202206202023SIRT1/APE1 promotes the viability of gastric cancer cells by inhibiting p53 to suppress ferroptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuijin Zhao,Yuanyi Ding,Lan ZhangPubMed 36820068
- Biomedical reports 18:92022Two weeks of high glucose intake is enough to induce intestinal mucosal damage and disturb the balance of the gut microbiota of rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCunyun Min,Tingting Fu,Wei Tan,Tingting Wang,Yu Du,Xuhui HuangPubMed 36570801
- International journal of molecular sciences 23:2022Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial-Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Ding,Yuning Yang,Qin Lu,Dongfeng Qu,Parthasarathy Chandrakesan,Hailan Feng,Hong Chen,Xuzheng Chen,Zhuhui Liao,Jian Du,Zhiyun Cao,Nathaniel WeygantPubMed 36362141
- Current issues in molecular biology 43:2210-22192021Simulated Microgravity Induces the Proliferative Inhibition and Morphological Changes in Porcine Granulosa Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTruong Xuan Dai,Hoang Nghia Son,Ho Nguyen Quynh Chi,Hoang Nghia Quang Huy,Nguyen Thai Minh,Nguyen Thi Thuy Tram,Nguyen Thi Thuong Huyen,To Minh Quan,Doan Chinh Chung,Truong Hai Nhung,Tran Thi Minh,Tran Hong Diem,Nguyen Thi Phuong Mai,Le Thanh LongPubMed 34940129
- Cancers 13:2021Inhibition of DCLK1 with DCLK1-IN-1 Suppresses Renal Cell Carcinoma Invasion and Stemness and Promotes Cytotoxic T-Cell-Mediated Anti-Tumor Immunity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Ding,Yuning Yang,Yang Ge,Qin Lu,Zixing Yan,Xuzheng Chen,Jian Du,Sassan Hafizi,Xiaohui Xu,Jiannan Yao,Jian Liu,Zhiyun Cao,Nathaniel WeygantPubMed 34830884
Key facts
- Applications
- ELISA, WB, ICC/IF, In-Cell ELISA
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity No | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info - | Notes - |
Application In-Cell ELISA | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
10X blocking buffer (pH 7.5) for serum-free blocking of non-specific antibody binding.
- Cited in over 30 publications
- Suitable for ELISA, in-cell ELISA, ICC/IF, western blot (WB)
- 100% casein blocking as preservative
Key facts
- Applications
- ELISA, WB, ICC/IF, In-Cell ELISA
- Form
- Liquid
- Storage buffer
Preservative: 100% Casein Blocking Buffer 10x
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- Ambient
- Appropriate long-term storage conditions
- Ambient
- Storage information
- The product can be stored for up to 12 months
Notes
Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot. BSA-free
Preparation: Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly.
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2 product images
Western blot - 10X Blocking Buffer (ab126587)
Primary :
All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000. Bottom membranes with Anti AMPKa (Anti-AMPK alpha 1 antibody [2B7] ab110036) at 1/1000
Lane 1 = AMPKa1 protein
Lane 2 = C2C12 cells treated with AICAR
Lane 3 = untreated C2C12 cells
Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.
Performed under reducing conditions (50mM DTT – Sample heated at 60°C). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa
Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST
Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes
Immunocytochemistry/ Immunofluorescence - 10X Blocking Buffer (ab126587)
HeLa cells were untreated or treated with 1mM Deferoxamine (DFO) for 24h and fixed with paraformaldehyde for imaging by fluorescent microscopy. Cells were blocked and stained with 1X blocking buffer (ab126587). Unpurified Anti-HIF-1 alpha antibody [EP1215Y] ab51608 was used at 1:500. DAPI was used to label the nucleus. HIF1 alpha staining is absent in untreated cells and induced by DFO treatment. HIF1 alpha localizes to the nucleus.
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