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Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot.

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Images

Western blot - 10X Blocking Buffer (AB126587), expandable thumbnail

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Key facts

Applications

ELISA, Flow Cyt, WB, ICC/IF, In-Cell ELISA

Form

Liquid

Reactivity data

Application

ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Application

Flow Cyt

Reactivity

No

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

-

Application

ICC/IF

Reactivity

Reacts

Dilution info

-

Notes

-

Application

In-Cell ELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Recommended products

Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot.

Key facts

Applications

ELISA, Flow Cyt, WB, ICC/IF, In-Cell ELISA

Form

Liquid

Storage buffer

Preservative: 100% Casein Blocking Buffer 10x

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate long-term storage conditions

Ambient

Storage information

The product can be stored for up to 12 months

Notes

Protein blocking buffer (pH 7.5) (ab126587) for serum-free blocking of non-specific antibody binding in ICC, ELISA and western blot. BSA-free

Preparation: Dilute to 1X in deionized water or PBS (e.g. 1mL 10X blocking buffer + 9mL water/PBS). Diluting in PBS instead of water makes a somewhat more stringent blocking buffer due to the presence of salt in the PBS. Blocking Buffer is pH 7.5. The user should determine empirically whether dilution in water or PBS is most appropriate for the assay at hand. Use diluted block solution promptly.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Western blot - 10X Blocking Buffer (ab126587), expandable thumbnail

    Western blot - 10X Blocking Buffer (ab126587)

    Primary :

    All Lanes : top membranes with Anti AMPK pThr172 + Thr183 (Anti-AMPK alpha 1 (phospho T183) + AMPK alpha 2 (phospho T172) antibody [EPR5683] ab133448) at 1/1000. Bottom membranes with Anti AMPKa (Anti-AMPK alpha 1 antibody [2B7] ab110036) at 1/1000

    Lane 1 = AMPKa1 protein

    Lane 2 = C2C12 cells treated with AICAR

    Lane 3 = untreated C2C12 cells

    Secondary: Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) and Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) at 1:10,000 developed using the ECL technique.

    Performed under reducing conditions (50mM DTT – Sample heated at 60°C). Predicted band size for AMPKa: 64 kDa Observed band size for AMPKa: 64 kDa

    Left membrane All blocking steps = 1X blocking reagent ab126587 diluted in PBST

    Right membrane All blocking steps = 5% Milk in PBST Exposure time : 5 minutes

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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