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EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (AB64216), expandable thumbnail

Publications

Key facts

Applications

IHC-P

Form

Liquid

Reactivity data

Application

IHC-P

Reactivity

Reacts

Dilution info

-

Notes

-

Recommended products

EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

Key facts

Applications

IHC-P

Form

Liquid

Storage buffer

pH: 7.7 - 8.1
Constituents: 0.29% EDTA

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Notes

EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

Recommended Procedure: Microwave

  1. Deparaffinize and rehydrate tissue sections.
  2. Dilute the product 1:10 with deionized water.
  3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
  4. Keep the slides warm by heating for 10min at LOW power.
  5. Let coplin jar sit in microwave for at least 20min.
  6. Remove and rinse with buffer.
  7. Proceed with staining.

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1 product image

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (ab64216)

    ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (Antibody Diluent ab64211)

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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