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AB64216

10x EDTA Buffer pH 8.0

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(5 Publications)

10X EDTA Buffer, pH 8.0 (ab64216) is suitable for heat-induced antigen retrieval (HIER) during immunohistochemistry (IHC) applications.

- Compatible with our Lab essentials imaging workflow offering
1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (AB64216)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - 10x EDTA Buffer pH 8.0 (AB64216)

ab64216 Human Melanoma stained with an anti-S-100 antibody diluted with Antibody Diluent (ab64211)

Key facts

Applications

IHC-P

applications

Form

Liquid

form

Storage buffer

pH: 7.7 - 8.1 Constituents: 0.29% EDTA

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

EDTA buffer pH 8.0 for heat-induced antigen retrieval (HIER) during IHC (ab64216).

Recommended Procedure: Microwave

  1. Deparaffinize and rehydrate tissue sections.
  2. Dilute the product 1:10 with deionized water.
  3. Immerse sections in coplin jar filled with target retrieval solution microwave on HIGH power until boiling.
  4. Keep the slides warm by heating for 10min at LOW power.
  5. Let coplin jar sit in microwave for at least 20min.
  6. Remove and rinse with buffer.
  7. Proceed with staining.
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:3838 PubMed38360830

2024

BAMLET administration via drinking water inhibits intestinal tumor development and promotes long-term health.

Applications

Unspecified application

Species

Unspecified reactive species

Hien Thi Tran,Murphy Lam Yim Wan,Ines Ambite,Michele Cavalera,Mario Grossi,Jaromir Háček,Parisa Esmaeili,António N B M Carneiro,Arunima Chaudhuri,Shahram Ahmadi,Catharina Svanborg

JCI insight 7: PubMed35015733

2022

Myocardial infarction reduces cardiac nociceptive neurotransmission through the vagal ganglia.

Applications

Unspecified application

Species

Unspecified reactive species

Siamak Salavatian,Jonathan D Hoang,Naoko Yamaguchi,Zulfiqar Ali Lokhandwala,Mohammed Amer Swid,John Andrew Armour,Jeffrey L Ardell,Marmar Vaseghi

Cellular and molecular gastroenterology and hepatology 13:113-127 PubMed34530178

2021

Nrf2 Mutation/Activation Is Dispensable for the Development of Chemically Induced Mouse HCC.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Mattu,Patrizia Zavattari,Marta Anna Kowalik,Marina Serra,Pia Sulas,Rajesh Pal,Elisabetta Puliga,Salvatore Sutti,Beatrice Foglia,Maurizio Parola,Emanuele Albano,Silvia Giordano,Andrea Perra,Amedeo Columbano

JCI insight 5: PubMed32493842

2020

Cardiac sympathetic activation circumvents high-dose beta blocker therapy in part through release of neuropeptide Y.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan D Hoang,Siamak Salavatian,Naoko Yamaguchi,Mohammed Amer Swid,Hamon David,Marmar Vaseghi

Laboratory investigation; a journal of technical m 100:986-1002 PubMed32054994

2020

Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Niké Posta,Éva Csősz,Melinda Oros,Dávid Pethő,László Potor,Gergő Kalló,Zoltán Hendrik,Katalin Éva Sikura,Gábor Méhes,Csaba Tóth,József Posta,György Balla,József Balla
View all publications
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