AB156034

10X RIPA Buffer

Name: 10X RIPA lysis buffer (pH 7.5) - 15 mL (ab156034) | Abcam
Brand: Abcam Limited
SKU: AB156034
Price: 75 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(246 Publications)

10X RIPA lysis buffer (pH 7.5) (ab156034) is used for cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells and tissues. Phosphatase inhibitors included in buffer formulation.

- Suitable buffer for your western blot SDS-PAGE, ELISA, IP and BCA assays
- Useful for lysis of whole-cell extracts and membrane-bound proteins
- Proven performance: cited in over 245 publications.
- Larger volume format available upon request

1 Images

SDS-PAGE

Unknown

SDS-PAGE - 10X RIPA Buffer (AB156034)

HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes : the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.

Lane 1 : MW marker
Lane 2 : Cleared lysate
Lane 3 : Non-soluble

Key facts

Applications

IP, SDS-PAGE, WB, ELISA

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 10% Nonylphenol, ethoxylated, 8.8% Sodium chloride, 6.1% Tris, 5% Sodium deoxycholate, 1.12% Sodium pyrophosphate decahydrate, 1% Sodium lauryl sulfate, 0.38% EGTA, 0.29% EDTA, 0.22% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.18% Sodium orthovanadate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suggested working concentration: 1X" } } }

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Product details

Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

Preparation: Dilute to 1X in deionized water

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Shipped at conditions

Ambient - Cannot Ship with Ice

Appropriate short-term storage conditions

Ambient

Appropriate long-term storage conditions

Ambient

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

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Publications (246)

Recent publications for all applications. Explore the full list and refine your search

Cytotechnology 77:137 PubMed40621114

2025

Mechanistic insights into Aloin-mediated regulation of STAT3/SLC7A11 signaling in head and neck squamous cell carcinoma.

Applications

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Species

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Li-Jian Wang,Jun Wang

NPJ precision oncology 9:224 PubMed40615519

2025

Genetic and oncogenic features of RASGRF fusions.

Applications

Unspecified application

Species

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Sreya Das,Daniel S Lenchner,Ellen Jaeger,Lisa Hunihan,Dana F DeSantis,Stamatina Fragkogianni,Karyn Ronski,Frederick H Wilson

Scientific reports 15:20470 PubMed40595762

2025

Polysaccharide from Prinsepia utilis Royle maintains the skin barrier by mediating differentiation, lipid metabolism and tight junction of keratinocyte.

Applications

Unspecified application

Species

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Ying Tu,Na Li,Hai-Yang Liu,Dong-Jie Sun,Li He,Hua Gu

Frontiers in pharmacology 16:1591394 PubMed40538548

2025

Aqueous extracts of L. alleviate atopic dermatitis by restoring skin barrier function.

Applications

Unspecified application

Species

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Haobin Wei,Zhang Chen,Wenjie Lai,Wenxian Wang,Xiqing Bian,Limin Zhang,Xinzhi Li

Methods in molecular biology (Clifton, N.J.) 2921:255-264 PubMed40515994

2025

Isolation of Exosome from Senescent Preadipocytes for Mass Spectrometric Analysis.

Applications

Unspecified application

Species

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Yao Wang

Genome research 35:1456-1471 PubMed40360186

2025

Charting the regulatory landscape of TP53 on transposable elements in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuan Qu,Yonghao Liang,Colin McCornack,Xiaoyun Xing,Heather Schmidt,Chad Tomlinson,Catrina Fronick,Edward A Belter,Juan F Macias-Velasco,Ting Wang

Nature communications 16:4393 PubMed40355429

2025

SARS-CoV-2 ORF3a drives dynamic dense body formation for optimal viral infectivity.

Applications

Unspecified application

Species

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Stella Hartmann,Lisa Radochonski,Chengjin Ye,Luis Martinez-Sobrido,Jueqi Chen

International journal of molecular sciences 26: PubMed40332529

2025

VEGF in Tears as a Biomarker for Exudative Age-Related Macular Degeneration: Molecular Dynamics in a Mouse Model and Human Samples.

Applications

Unspecified application

Species

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Seyed Mohamad Mehdi Moshtaghion,Filippo Locri,Alvaro Plaza Reyes,Flavia Plastino,Anders Kvanta,Maria Jose Morillo-Sanchez,Enrique Rodríguez-de-la-Rúa,Estanislao Gutierrez-Sanchez,Adoración Montero-Sánchez,Helena Lucena-Padros,Helder André,Francisco J Díaz-Corrales

Cell & bioscience 15:55 PubMed40281637

2025

Zmynd11 is essential for neurogenesis by coordinating H3K36me3 modification of Epha2 and PI3K signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Yang,Lan Li,Wenzheng Qu,Xuejun Cheng,Jinyu Zhang,Yan Sun,Suxiao Liu,Guoping Peng,Rui Zheng,Xuekun Li

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2409040 PubMed40245004

2025

α-Synuclein Pathology Spreads in a Midbrain-Hindbrain Assembloid Model.

Applications

Unspecified application

Species

Unspecified reactive species

Gemma Gomez-Giro,Daniela Frangenberg,Daniela Vega,Alise Zagare,Kyriaki Barmpa,Paul M A Antony,Graham Robertson,Rahman Sabahi-Kaviani,Kristian Haendler,Nathalie Kruse,Florentia Papastefanaki,Rebecca Matsas,Malte Spielmann,Regina Luttge,Jens C Schwamborn

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

10X RIPA Buffer

SDS-PAGE - 10X RIPA Buffer (AB156034)

Key facts

Applications

Form

Storage buffer

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Multiplex Kits

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (246)

Product promise