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10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells.

- Cited in over 180 publications
- Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot
- Effectively extracts cytoplasmic, nuclear and membrane proteins


Images

SDS-PAGE - 10X RIPA Buffer (AB156034), expandable thumbnail

Publications

Key facts

Applications
IP, ELISA, SDS-PAGE, WB
Form
Liquid

Reactivity data

Application
IP
Reactivity
Reacts
Dilution info
-
Notes

Suggested working concentration: 1X

Application
ELISA
Reactivity
Reacts
Dilution info
-
Notes

Suggested working concentration: 1X

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

Suggested working concentration: 1X

Application
WB
Reactivity
Reacts
Dilution info
-
Notes

Suggested working concentration: 1X

Recommended products

10X RIPA lysis buffer (pH 7.5) is a means of cell lysis and protein solubilization for both adherent and suspension-cultured mammalian cells.

- Cited in over 180 publications
- Suitable for immunoprecipitation (IP), ELISA, SDS-PAGE, western blot
- Effectively extracts cytoplasmic, nuclear and membrane proteins

Key facts

Applications
IP, ELISA, SDS-PAGE, WB
Form
Liquid
Storage buffer

pH: 7.5
Constituents: 10% Nonylphenol, ethoxylated, 8.8% Sodium chloride, 6.1% Tris, 5% Sodium deoxycholate, 1.12% Sodium pyrophosphate decahydrate, 1% Sodium lauryl sulfate, 0.38% EGTA, 0.29% EDTA, 0.22% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.18% Sodium orthovanadate

Storage

Shipped at conditions
Ambient - Cannot Ship with Ice
Appropriate short-term storage conditions
Ambient
Appropriate long-term storage conditions
Ambient

Notes

Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.

Preparation: Dilute to 1X in deionized water

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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Terms & Conditions.

1 product image

  • SDS-PAGE - 10X RIPA Buffer (ab156034), expandable thumbnail

    SDS-PAGE - 10X RIPA Buffer (ab156034)

    HeLa cell extraction using ab156034.

    2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.

    BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.

    Lane 1: MW marker
    Lane 2: Cleared lysate
    Lane 3: Non-soluble

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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