Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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Publications
- World journal of oncology 15:100-1132024Inhibiting the mA Reader IGF2BP3 Suppresses Ovarian Cancer Cell Growth via Regulating PLAGL2 mRNA Stabilization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTian Tian Dai,Yi Ze Li,Hui Ting Hu,Yong Mei Zhao,Hong Yan Peng,Wen Dong Bai,Jing Wen WangPubMed 38274714
- NPJ precision oncology 8:32024A destabilizing Y891D mutation in activated EGFR impairs sensitivity to kinase inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel S Lenchner,Zaritza O Petrova,Lisa Hunihan,Kumar D Ashtekar,Zenta Walther,Frederick H WilsonPubMed 38182677
Key facts
- Applications
IP, ELISA, SDS-PAGE, WB
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IP | Reactivity Reacts | Dilution info - | Notes Suggested working concentration: 1X |
Application ELISA | Reactivity Reacts | Dilution info - | Notes Suggested working concentration: 1X |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Suggested working concentration: 1X |
Application WB | Reactivity Reacts | Dilution info - | Notes Suggested working concentration: 1X |
Recommended products
Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
Key facts
- Applications
IP, ELISA, SDS-PAGE, WB
- Form
Liquid
- Storage buffer
pH: 7.5
Constituents: 10% Nonylphenol, ethoxylated, 8.8% Sodium chloride, 6.1% Tris, 5% Sodium deoxycholate, 1.12% Sodium pyrophosphate decahydrate, 1% Sodium lauryl sulfate, 0.38% EGTA, 0.29% EDTA, 0.22% Beta glycerophosphate, 0.18% Sodium orthovanadate
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
Notes
Abcam's 10X RIPA lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent effectively extracts cytoplasmic, nuclear and membrane proteins. It is compatible with many downstream applications, including SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
Preparation: Dilute to 1X in deionized water
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1 product image
SDS-PAGE - 10X RIPA Buffer (ab156034)
HeLa cell extraction using ab156034.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156034. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.1mg of protein was recovered and 82% was in the soluble cleared cell lysate.
Lane 1: MW marker
Lane 2: Cleared lysate
Lane 3: Non-soluble
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