Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
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- eLife 9:2020Tissue-resident macrophages promote extracellular matrix homeostasis in the mammary gland stroma of nulliparous mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Wang et. al.PubMed 32479261
- Nature communications 11:2202020Arf1-mediated lipid metabolism sustains cancer cells and its ablation induces anti-tumor immune responses in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGuohao Wang et. al.PubMed 31924786
- BMC complementary and alternative medicine 16:3102016Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model.Applications:IHC-PReactive species:Unspecified reactive speciesChao-Wei Huang,Tzu-Wen Hong,Ying-Jing Wang,Ko-Chien Chen,Ju-Chun Pei,Tai-Yuan Chuang,Wen-Sung Lai,Sheng-Hong Tsai,Richard Chu,Wei-Cheng Chen,Lee-Yan Sheen,Satoru Takahashi,Shih-Torng Ding,Tang-Long ShenPubMed 27553852
- Molecular medicine reports 10:737-422014Elevated expression of AKT2 correlates with disease severity and poor prognosis in human osteosarcoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYalong Zhu,Jianhua Zhou,Ying Ji,Baoqing YuPubMed 24919955
Key facts
- Applications
IHC-P
- Form
Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
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Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step.
Key facts
- Applications
IHC-P
- Form
Liquid
- Storage buffer
pH: 8
Constituents: 5% Tetrasodium ethylene diamine tetraacetate, 1.5% 2-butoxyethanol
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
Ambient
- Appropriate long-term storage conditions
Ambient
Notes
Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) is a special solution that enables rehydrate and target retrieval in formalin-fixed, paraffin-embedded tissue sections in one step. It is optimal for use with primary antibodies that require EDTA buffer (pH=8.0) pretreatment.
This product contains detergent for emulsification of the paraffin.
1X Dilution: The 100X stock solution should be diluted 100-fold with distilled water before use.
PROTOCOL:
- Place paraffin-embedded slides in 1x Antigen Retrieval Buffer; cover with a vented plastic wrap, place in microwave and set high power to boil and then set low power to keep it boiling for 10 min. Let the sections cool in the microwave for at least 20min.
- Wash sections with hot tap water for 1 minute.
- Wash sections in buffer for 2x3 minutes.
- Continue with IHC protocol as normal.
Other kits and reagents for IHC
Other antigen retrieval buffers include:
Citrate buffer pH 6.0 ab93678, Tris-EDTA buffer pH 9.0 ab93684, Tris buffer pH 10.0 ab93682, or see the full list of antigen retrieval buffers and enzymes.
Find more kits and reagents for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)
Prolonged survival of Lgr5/Apc and MYC-ON mice depends on the T cells.g Immunohistochemical staining of GFP in the indicated genotypes (n = 5 mice per group).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)
Ophiocordyceps formosana upregulated adiponectin expressions in plasma and adipose tissuesb) Immunohistochemical staining for adiponectin in adipose tissues.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X EDTA Buffer, pH 8.0) (ab93680)
ab93680 Antigen Retrieval Buffer 100X EDTA Buffer, pH 8
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