Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0)

35 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling LINE-1 ORF1p with Anti-LINE-1 ORF1p antibody [EPR21844-108] ab216324 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on spermatogonia of mouse testis (PMID: 24607009) is observed. Counterstained with hematoxylin.
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
  Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical for RUNX2 on murine omental metastasis Antigen retrieval: Tris-EDTA, pH;9 (ab93684), Blocking: Normal Goat Serum, Primary: Rabbit monoclonal anti-Runx2 [EPR14334] (Anti-RUNX2 antibody [EPR14334] ab192256), dilution 1:1000, Secondary: HRP anti-rabbit

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  ab93684 Antigen Retrieval Buffer 100X Tris-EDTA Buffer, pH 9

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling Met (c-Met) - C-terminal with Alexa Fluor® 488 Anti-Met (c-Met) - C-terminal antibody [SP44] ab314588 at 1/100 (5.0 ug/ml).
  Positive staining on human colon cancer.
  The section was incubated with Alexa Fluor® 488 Anti-Met (c-Met) - C-terminal antibody [SP44] ab314588 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Met (c-Met) - C-terminal with Alexa Fluor® 488 Anti-Met (c-Met) - C-terminal antibody [SP44] ab314588 at 1/100 (5.0 ug/ml).
  Positive staining on human colon.
  The section was incubated with Alexa Fluor® 488 Anti-Met (c-Met) - C-terminal antibody [SP44] ab314588 at 4°C overnight (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling EBI3 with Alexa Fluor® 555 Anti-EBI3 antibody [EPR25341-155] ab314592 at 1/50 (10.0 ug/ml).
  Cytoplasmic staining on human placental trophoblasts.
  The section was incubated with Alexa Fluor® 555 Anti-EBI3 antibody [EPR25341-155] ab314592 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Wilms Tumor Protein with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 1/100 (5.0 ug/ml).
  Negative control: no staining on human skeletal muscle.
  The section was incubated with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Rat testis tissue labeling Wilms Tumor Protein with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 1/100 (5.0 ug/ml).
  Nuclear staining on rat testis.
  The section was incubated with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling Wilms Tumor Protein with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 1/100 (5.0 ug/ml).
  Nuclear staining on mouse testis.
  The section was incubated with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Wilms Tumor Protein with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 1/100 (5.0 ug/ml).
  Nuclear staining on human ovarian cancer.
  The section was incubated with Alexa Fluor® 555 Anti-Wilms Tumor Protein antibody [CAN-R9(IHC)-56-2] ab314587 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.
  Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling Alpha-synuclein with HRP Anti-Alpha-synuclein antibody [MJFR1] ab314243 at 1/100 (5.0 µg/ml).
  Negative control: no staining on human skeletal muscle.
  The section was incubated with HRP Anti-Alpha-synuclein antibody [MJFR1] ab314243 at 4°C overnight.
  Counterstained with Hematoxylin.
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Alpha-synuclein with HRP Anti-Alpha-synuclein antibody [MJFR1] ab314243 at 1/100 (5.0 µg/ml).
  Cytoplasmic staining on human cerebrum.
  The section was incubated with HRP Anti-Alpha-synuclein antibody [MJFR1] ab314243 at 4°C overnight.
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Mannose Receptor with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 1/50 (10.0 ug/ml) . Membranous staining on stormal cells in human liver. The section was incubated with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Mannose Receptor with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 1/50 (10.0 ug/ml) . Membranous staining on immune cells in human colon. The section was incubated with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Mannose Receptor with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 1/50 (10.0 ug/ml) . Negative control: no staining on human skeletal muscle. The section was incubated with Alexa Fluor® 555 Anti-Mannose Receptor antibody [EPR22489-7] ab313731 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human cerebral cortex (PMID: 24114376) is observed. Counter stained with hematoxylin.
  

  
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  

  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling FABP5 with Anti-FABP5 antibody [EPR22552-64] ab255276 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on blood vessels of human placenta (PMID: 19625659) is observed. Counter stained with hematoxylin.
  

  
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
  

  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded mouse brain labeling Dopamine Receptor D1 with anti dopamine receptor d1 antibody 11f11 se6 ihcp mouse cerebrumat 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in corpus striatum of mouse brain was observed. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded rat corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in rat corpus striatum was observed. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded mouse corpus striatum labeling Dopamine Receptor D1 with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 1/500 dilution (2.174 µg/ml). The section was incubated with Anti-Dopamine Receptor D1 antibody [1-1-F11 s.E6] ab256555 at 4°C overnight followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) secondary antibody (Goat Anti-Rat IgG H&L (HRP polymer) ab214882). Positive staining in mouse corpus striatum was observed. Counterstained with Hematoxylin.
  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SMARCA2 / BRM with Alexa Fluor® 488 Anti-SMARCA2 / BRM antibody [EPR23103-44] ab307770 at 1/100 dilution (5.0 µg/ml).
  Nuclear staining on mouse cerebrum.
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SMARCA2 / BRM with Alexa Fluor® 488 Anti-SMARCA2 / BRM antibody [EPR23103-44] ab307770 at 1/100 dilution (5.0 µg/ml).
  Nuclear staining on human cerebrum.
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling SMARCA2 / BRM with Alexa Fluor® 647 Anti-SMARCA2 / BRM antibody [EPR23103-44] ab307771 at 1/100 dilution (5.0 µg/ml).
  Nuclear staining on human cerebrum.
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling SMARCA2 / BRM with Alexa Fluor® 647 Anti-SMARCA2 / BRM antibody [EPR23103-44] ab307771 at 1/100 dilution (5.0 µg/ml).
  Nuclear staining on mouse cerebrum.
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in red). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling LC3B with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 1/100 dilution (5.0 µg/ml).
  
  Cytoplasmic staining on human cerebrum.
  
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling LC3B with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 1/100 dilution (5.0 µg/ml).
  Cytoplasmic staining on human glioma.
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling LC3B with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 1/100 dilution (5.0 µg/ml).
  
  Negative control: no staining on human stomach.
  
  
  The section was incubated with Alexa Fluor® 555 Anti-LC3B - Autophagosome Marker antibody [EPR18709] ab307768 at 4°C overnight (shown in red). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human astrocytoma labelling Pan Trk with Anti-Pan Trk antibody [EPR17341-4] ab246551 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
  
  Positive staining on human astrocytoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human breast carcinoma labelling Niemann Pick C2 with Anti-Niemann Pick C2 antibody [EPR19993-145-1] ab218192 at 1/8000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
  
  Positive staining on Human breast carcinoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with Anti-CD10 antibody [EPR22867-118] ab256494 at 1/500 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
  
  Positive staining on Human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma labelling CD10 with Anti-CD10 antibody [EPR22865-73] ab255609 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Positive staining on human diffuse large B-cell lymphoma is observed. Counter stained with hematoxylin.
  
  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a Goat Anti-Rabbit IgG H&L (HRP polymer) ready to use.
  
  Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling CD15 with Alexa Fluor® 488 Anti-CD15 antibody [EPR9521] ab313399 at 1/100 (5.0 ug/ml). Negative control: no staining on human skeletal muscle. The section was incubated with Alexa Fluor® 488 Anti-CD15 antibody [EPR9521] ab313399 at 4°C overnight (shown in green). Nuclear DNA was labeled with DAPI (shown in blue). The section was then mounted using Fluoromount®. The image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemistry analysis of paraffin-embedded mouse lymph node tissue section labelling CSF-1-R with Anti-CSF-1-R antibody [YEYE311] ab271294 at 1/200 dilution. The section was incubated with Anti-CSF-1-R antibody [YEYE311] ab271294 at +4°C overnight. Goat Anti-Rat IgG H&L (HRP polymer) (Goat Anti-Rat IgG H&L (HRP polymer) ab214882) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Positive staining on the macrophages in mouse lymph node. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) (ab93684)

  Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling SCP3 with Anti-SCP3 antibody [EPR27086-101] ab314313 at 1/100 (5.02 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880). Negative control: no staining on rat lung. The section was incubated with Anti-SCP3 antibody [EPR27086-101] ab314313 at 4°C overnight. Counterstained with Hematoxylin.
  Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer) (Goat Anti-Rabbit IgG H&L (HRP polymer) ab214880).
  Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0).