AB152163

Cell Lysis Buffer

Name: Cell Lysis Buffer (ab152163) | Abcam
Brand: Abcam Limited
SKU: AB152163
Price: 140 USD
Availability: InStock

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(35 Publications)

Cell Lysis Buffer (ab152163) is 10X stock buffer for cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells under non-denatured conditions. Suitable for western blot and IP applications.

- Proven performance: cited in over 30 publications

Key facts

Applications

WB, IP

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 10% Triton X-100, 8.8% Sodium chloride, 3.15% Tris HCl, 1.12% Sodium pyrophosphate decahydrate, 0.38% EGTA, 0.29% Tetrasodium ethylene diamine tetraacetate, 0.216% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate, 0.19% Sodium orthovanadate, 0.001% Leupeptin

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

ab152163 is a 10X Cell Lysis Buffer used to lyse cells under non-denaturing conditions.

Recommended for preparing samples for use in western blotting and immunoprecipitation. For use with adherent or suspension cells.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

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Target data

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Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Journal of cosmetic dermatology 24:e70228 PubMed40353553

2025

Platelet-Released Growth Factors (PRGFs) Activate NRF2-ARE and Modulate Inflammatory Response in an NRF2-Dependent Manner in Primary Human Keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Matthias Stein,Nicole Böttcher,Mersedeh Tohidnezhad,Athanassios Fragoulis,Andreas Bayer,Hannes Klump,Jens M Baron,Thomas Pufe

International journal of molecular sciences 26: PubMed39859163

2025

Alterations in Autophagic Function and Endoplasmic Reticulum Stress Markers in the Peripheral Blood Mononuclear Cells of Patients on Hemodialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Chih Liu,Ming-Yin Wu,Paik Seong Lim

Science advances 10:eadp7022 PubMed39485841

2024

Reprogramming cellular senescence in the tumor microenvironment augments cancer immunotherapy through multifunctional nanocrystals.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Wang,Yinglu Chen,Hui Fang,Kai Xiao,Ziping Wu,Xiaochun Xie,Jie Liu,Fangman Chen,Yi He,Liang Wang,Chao Yang,Renjun Pei,Dan Shao

Macromolecular bioscience 24:e2300414 PubMed38035771

2023

In Vitro and In Vivo Evaluation of 3D Printed Poly(Ethylene Glycol) Dimethacrylate-Based Photocurable Hydrogel Platform for Bone Tissue Engineering.

Applications

Unspecified application

Species

Unspecified reactive species

Janitha M Unagolla,Bipin Gaihre,Ambalangodage C Jayasuriya

Life sciences 321:121598 PubMed36963720

2023

Collagen matricryptin promotes cardiac function by mediating scar formation.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel A Grilo,Sirin N Cakir,Patti R Shaver,Rugmani P Iyer,Kaitlin Whitehead,Joseph M McClung,Ali Vahdati,Lisandra E de Castro Brás

International journal of molecular sciences 23: PubMed36012289

2022

Increased Epidermal Nerve Growth Factor without Small-Fiber Neuropathy in Dermatomyositis.

Applications

Unspecified application

Species

Unspecified reactive species

Lai-San Wong,Chih-Hung Lee,Yu-Ta Yen

Cells 10: PubMed33919736

2021

Periostin Short Fragment with Exon 17 via Aberrant Alternative Splicing Is Required for Breast Cancer Growth and Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuka Ikeda-Iwabu,Yoshiaki Taniyama,Naruto Katsuragi,Fumihiro Sanada,Nobutaka Koibuchi,Kana Shibata,Kenzo Shimazu,Hiromi Rakugi,Ryuichi Morishita

Journal of virology 95: PubMed33536170

2021

Nonsegmented Negative-Sense RNA Viruses Utilize -Methyladenosine (mA) as a Common Strategy To Evade Host Innate Immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Mijia Lu,Miaoge Xue,Hai-Tao Wang,Elizabeth L Kairis,Sadeem Ahmad,Jiangbo Wei,Zijie Zhang,Qinzhe Liu,Yuexiu Zhang,Youling Gao,Dominique Garcin,Mark E Peeples,Amit Sharma,Sun Hur,Chuan He,Jianrong Li

Scientific reports 11:5687 PubMed33707502

2021

Chlorite oxidized oxyamylose differentially influences the microstructure of fibrin and self assembling peptide hydrogels as well as dental pulp stem cell behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Mostafa EzEldeen,Burak Toprakhisar,Denise Murgia,Nick Smisdom,Olivier Deschaume,Carmen Bartic,Hans Van Oosterwyck,Rafaela Vaz Sousa Pereira,Ghislain Opdenakker,Ivo Lambrichts,Annelies Bronckaers,Reinhilde Jacobs,Jennifer Patterson

BMC cardiovascular disorders 21:51 PubMed33526034

2021

CircTM7SF3 contributes to oxidized low-density lipoprotein-induced apoptosis, inflammation and oxidative stress through targeting miR-206/ASPH axis in atherosclerosis cell model in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Wang,Ming Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Cell Lysis Buffer

Key facts

Applications

Form

Storage buffer

Reactivity data

Product details

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (35)

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Product promise