Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
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- Translational pediatrics 12:1139-11472023Exploring dynamic change and influencing factors of TGF-β1 and MUC1 in human breast milk, and their association with infantile diseases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Wu,Yue Li,Xiao-Ya Su,Tong-Xin ChenPubMed 37427064
- Molecular cell 82:3045-3060.e112022Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan He,Huijin Feng,Belen Sundberg,Jiaxing Yang,Justin Powers,Alec H Christian,John E Wilkinson,Cian Monnin,Daina Avizonis,Craig J Thomas,Richard A Friedman,Michael D Kluger,Michael A Hollingsworth,Paul M Grandgenett,Kelsey A Klute,F Dean Toste,Christopher J Chang,Iok In Christine ChioPubMed 35752173
- Autophagy 19:92-1112022Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUrvi Gupta,Sayan Ghosh,Callen T Wallace,Peng Shang,Ying Xin,Archana Padmanabhan Nair,Meysam Yazdankhah,Anastasia Strizhakova,Mark A Ross,Haitao Liu,Stacey Hose,Nadezda A Stepicheva,Olivia Chowdhury,Mihir Nemani,Vishnu Maddipatla,Rhonda Grebe,Manjula Das,Kira L Lathrop,José-Alain Sahel,J Samuel Zigler,Jiang Qian,Arkasubhra Ghosh,Yuri Sergeev,James T Handa,Claudette M St Croix,Debasish SinhaPubMed 35473441
- Molecular medicine reports 18:5511-55192018Syringaresinol‑di‑O‑β‑D‑glucoside, a phenolic compound from Polygonatum sibiricum, exhibits an antidiabetic and antioxidative effect on a streptozotocin‑induced mouse model of diabetes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiping Zhai et. al.PubMed 30365054
Key facts
- Applications
- WB, SDS-PAGE, IP, sELISA
- Form
- Liquid
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application IP | Reactivity Reacts | Dilution info - | Notes - |
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Recommended products
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells.
Key facts
- Applications
- WB, SDS-PAGE, IP, sELISA
- Form
- Liquid
- Storage buffer
pH: 7.5
Constituents: 1.5% Dodecyl maltoside, 0.6% HEPES, 0.58% Sodium chloride, 0.11% Sodium pyrophosphate decahydrate, 0.04% EGTA, 0.03% EDTA, 0.02% Sodium orthovanadate, 0.02% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent extracts cytoplasmic, nuclear and membrane proteins. The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity. On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g. 10X RIPA Buffer ab156034). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
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SDS-PAGE - Native lysis Buffer (ab156035)
HeLa cell extraction using ab156035.
2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156035. Next the sample was centrifuged at 14,000 rpm at 4ºC for 15 minutes: the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.
BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.15mg of protein was recovered and 70% was in the soluble cleared cell lysate.
Lane 1: MW marker
Lane 2: Cleared lysate
Lane 3: Non-soluble
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