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AB156035

Native lysis Buffer

5

(1 Review)

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(8 Publications)

Native lysis buffer (ab156035) is a ready-to-use buffer for cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells under non-denatured conditions.

- Suitable for SDS-PAGE, sELISA, IP and western blot applications
- Compatible with our Lab essentials western blot staining workflow
1 Images
SDS-PAGE - Native lysis Buffer (AB156035)
  • SDS-PAGE

Unknown

SDS-PAGE - Native lysis Buffer (AB156035)

HeLa cell extraction using ab156035.

2.5 million HeLa cells were lysed on ice for 15 minutes with 0.5 mL of 1X ab156035. Next the sample was centrifuged at 14,000 rpm at 4°C for 15 minutes : the supernatant ( = cleared lysate) was removed and the pellet ( = insoluble material) was resuspended in 0.5 mL lysis buffer and solubilized by sonication. Equivalent loads of the cleared lysate and solubilized pellet were analyzed by SDS-PAGE and Coomassie stain.

BCA protein concentration determination of the soluble and insoluble material indicates that a total of 1.15mg of protein was recovered and 70% was in the soluble cleared cell lysate.

Lane 1 : MW marker
Lane 2 : Cleared lysate
Lane 3 : Non-soluble

Key facts

Applications

sELISA, SDS-PAGE, WB, IP

applications

Form

Liquid

form

Storage buffer

pH: 7.5 Constituents: 1.5% Dodecyl maltoside, 0.6% HEPES, 0.58% Sodium chloride, 0.11% Sodium pyrophosphate decahydrate, 0.04% EGTA, 0.03% EDTA, 0.02% Sodium orthovanadate, 0.02% disodium;1,3-dihydroxypropan-2-yl phosphate;hydrate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IP": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam’s 1X Native lysis buffer is an efficient means of cell lysis and protein solubilization for both adherent and suspension cultured mammalian cells. This reagent extracts cytoplasmic, nuclear and membrane proteins. The detergent used in this buffer is relatively gentle and in many cases allows solubilization of protein complexes that retain enzymatic activity. On the flip side, the gentle nature of this lysis buffer means that it may result in extractions that are less complete than lysis buffers using more stringent detergents (e.g. ab156034). Native lysis buffer is compatible with many downstream applications, including enzyme activity assays, SDS-PAGE, Western blot, immunoprecipitation, ELISA and BCA assays.
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PLoS pathogens 21:e1012985 PubMed40067802

2025

Enhancing MyD88 oligomerization is one important mechanism by which IBDV VP2 induces inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Mengmeng Huang,Mengmeng Xu,Jingzhe Han,Erjing Ke,Xinxin Niu,Yulong Zhang,Guodong Wang,Hangbo Yu,Runhang Liu,Suyan Wang,Yongzhen Liu,Yuntong Chen,Jinze Han,Ziwen Wu,Hongyu Cui,Yanping Zhang,Yulu Duan,Yulong Gao,Xiaole Qi

iScience 27:111354 PubMed39640585

2024

HucMSCs can alleviate abnormal vasculogenesis induced by high glucose through the MAPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yao,Tiantian Shan,Xiaoying Li

International journal of molecular sciences 25: PubMed39063228

2024

Discovery of Cell-Permeable Allosteric Inhibitors of Liver Pyruvate Kinase: Design and Synthesis of Sulfone-Based Urolithins.

Applications

Unspecified application

Species

Unspecified reactive species

Shazia Iqbal,Md Zahidul Islam,Sajda Ashraf,Woonghee Kim,Amal A Al-Sharabi,Mehmet Ozcan,Essam Hanashalshahaby,Cheng Zhang,Mathias Uhlén,Jan Boren,Hasan Turkez,Adil Mardinoglu

Translational pediatrics 12:1139-1147 PubMed37427064

2023

Exploring dynamic change and influencing factors of TGF-β1 and MUC1 in human breast milk, and their association with infantile diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wu,Yue Li,Xiao-Ya Su,Tong-Xin Chen

American journal of Alzheimer's disease and other dementias 37:15333175221124949 PubMed36113018

2022

Acetoacetate Improves Memory in Alzheimer's Mice via Promoting Brain-Derived Neurotrophic Factor and Inhibiting Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jun Wu,Qin-Qin Shu,Bin Wang,Lan Dong,Bin Hao

Molecular cell 82:3045-3060.e11 PubMed35752173

2022

Methionine oxidation activates pyruvate kinase M2 to promote pancreatic cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan He,Huijin Feng,Belen Sundberg,Jiaxing Yang,Justin Powers,Alec H Christian,John E Wilkinson,Cian Monnin,Daina Avizonis,Craig J Thomas,Richard A Friedman,Michael D Kluger,Michael A Hollingsworth,Paul M Grandgenett,Kelsey A Klute,F Dean Toste,Christopher J Chang,Iok In Christine Chio

Autophagy 19:92-111 PubMed35473441

2022

Increased LCN2 (lipocalin 2) in the RPE decreases autophagy and activates inflammasome-ferroptosis processes in a mouse model of dry AMD.

Applications

Unspecified application

Species

Unspecified reactive species

Urvi Gupta,Sayan Ghosh,Callen T Wallace,Peng Shang,Ying Xin,Archana Padmanabhan Nair,Meysam Yazdankhah,Anastasia Strizhakova,Mark A Ross,Haitao Liu,Stacey Hose,Nadezda A Stepicheva,Olivia Chowdhury,Mihir Nemani,Vishnu Maddipatla,Rhonda Grebe,Manjula Das,Kira L Lathrop,José-Alain Sahel,J Samuel Zigler,Jiang Qian,Arkasubhra Ghosh,Yuri Sergeev,James T Handa,Claudette M St Croix,Debasish Sinha

Molecular medicine reports 18:5511-5519 PubMed30365054

2018

Syringaresinol‑di‑O‑β‑D‑glucoside, a phenolic compound from Polygonatum sibiricum, exhibits an antidiabetic and antioxidative effect on a streptozotocin‑induced mouse model of diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhai,Xu Wang
View all publications
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